LOCUS       Nup58_mEGFP(-7)         6501 bp ds-DNA     linear       04-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        4046..4362
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4166..4362
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1661..1909
                     /label="Nup54 Interacting Domain??"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1913..2611
                     /label="a-helical region"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3549..3855)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    680..3124
                     /label="NUP58"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      5723..6405
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    680..1396
                     /label="FG Domain"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1910..2611
                     /label="mEGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    672..679
                     /label=""
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PCR_primer      complement(3137..3155)
                     /label=""
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4400..5191
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     PCR_primer      526..551
                     /label="GGGCGGTAGGCGTGTACGGTGGGAGG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     PCR_primer      512..536
                     /label="CCATTGACGCAAATGGGCGGTAGGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1397..1909
                     /label="alpha-helical region"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2612..3121
                     /label="FG Domain"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          1203..1222
                     /label="N58_seqprime2"
                     /note="sequence: caacatcaggtctcggacag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(3189..3208)
                     /label="pFastBacR"
                     /note="sequence: caaatgtggtatggctgatt"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcccgcgaca tggccacggg gttctccttt gggtccggga ccctcggctc
      721 caccactgtg gcccccggcg gcaccggcac aggcagcgga ttctccttcg gcgcgtccag
      781 cacaccttct gtggggctca acttcggaac tctcggaagc tctgcgacac cagcatctac
      841 atctacttct gccagtggtt ttggaacagg actctttgga tctaagcctg gcactggatt
      901 cactctagga ggaacaagtg caggaacaac agctactaca tctgcatcta caacaggctt
      961 tagtttagga ttcagtaaac ctgcagcatc tgccacacca tttgccctgc ccgtcacttc
     1021 taccacagct agtggtctta ctctctcgtc tgctctgaca tcagctccag cagcatccac
     1081 aggatttacg ttgaataact tgggagcaac accagccaca actacagcag catcaacagg
     1141 actttcctta gggggggcct tagctggttt gggaggttca cttttccaga gtggaaacac
     1201 agcaacatca ggtctcggac agaatgcttt aagcttgtct ctggggactg caacacctac
     1261 ttcagcagcc agcagtgaag gccttggtgg tatagatttt agtacctcat cagataaaaa
     1321 gagtgataaa acgggaacaa ggccagagga tagcaaagcc ctgaaagatg aaaaccttcc
     1381 tcctgtcatc tgtcaggatg ttgagaatct ccagaaattt gtcaaggaac aaaaacaagt
     1441 ccaagaagaa attagtcgaa tgtcttccaa agcaatgctt aaagtccagg aggacatcaa
     1501 ggccctgaag cagctcctgt cattggctgc tagtgggttg cagaggaaca cactcaatat
     1561 tgacaaactg aagttagaaa ctgctcagga gttaaagaat gcggaaatag ctttaagaac
     1621 ccagaaaaca ccacctggac tccaacatga aaatacagct cctgctgact acttcagagt
     1681 cttggttcag caatttgagg tacagcttca gcagtacagg cagcagattg aagaactaga
     1741 aaaccatctt gccactcaag caaacaattc acatataact ccgcaagact tatcaatggc
     1801 tatgcagaaa atttatcaaa catttgtagc tttagctgca caacttcagt ccattcatga
     1861 aaatgtcaag gtgcttaaag aacagtatct cagctacaga aaaatgtttG AGGAGCTGTT
     1921 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG
     1981 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG
     2041 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT
     2101 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT
     2161 GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC
     2221 CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT
     2281 CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA
     2341 CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG
     2401 CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT
     2461 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAG
     2521 CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG
     2581 GATCACTCTC GGCATGGACG AGCTGTACAA Ggatgtgttt gaagccagga gaacagaagc
     2641 caagaagtgg cagaatgcac ccagagttac tactggaccc acgcctttca gcaccatgcc
     2701 aaacgcagca gccgttgcca tggctgcaac acttacacag cagcaacagc ctgctacagg
     2761 gccacagccg tctctgggag ttagttttgg aacgccattc ggctcaggta ttggcactgg
     2821 cttgcaatca agtggcttag gttcttcaaa ccttggagga tttggaacta gctctggttt
     2881 cggatgcggt accacagggg cctccacatt tggatttgga acaacagata aaccctcagg
     2941 aagtcttagt gcaggctttg gcagctcaag tacgtctggg tttaacttca gcaatcctgg
     3001 catcacggca tcagctggtt tgacatttgg ggtgtccaat cctgcgtctg cggggtttgg
     3061 aacaggagga caactccttc agttgaagag acctcccgct ggaaacaaaa gaggaaagag
     3121 atgaccattg cgctgtccag aggttcataa agctgtgtac aagtaaagcg gccgcgactc
     3181 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3241 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     3421 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3481 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3541 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3601 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     3661 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3721 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3781 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3841 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     3901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     4021 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     4081 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     4141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     4201 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     4261 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     4321 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     4381 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     4441 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     4501 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     4561 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     4621 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     4681 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     4741 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     4801 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     4861 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     4921 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     4981 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5041 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     5101 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     5161 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     5221 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     5281 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     5341 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     5401 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     5461 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     5521 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     5581 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     5641 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5941 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6421 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6481 aaccgtatta ccgccatgca t
//