LOCUS       Nup58_mEGFP(-8)         6498 bp ds-DNA     linear       04-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             4397..5188
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     PCR_primer      526..551
                     /label="GGGCGGTAGGCGTGTACGGTGGGAGG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        4043..4359
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        4163..4359
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1661..1906
                     /label="Nup54 Interacting Domain??"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     PCR_primer      512..536
                     /label="CCATTGACGCAAATGGGCGGTAGGC"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    672..679
                     /label=""
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1397..1906
                     /label="alpha-helical region"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1907..2608
                     /label="mEGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      5720..6402
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    680..3121
                     /label="NUP58"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     PCR_primer      complement(3134..3152)
                     /label=""
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    680..1396
                     /label="FG Domain"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1910..2608
                     /label="a-helical region"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(3546..3852)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2609..3118
                     /label="FG Domain"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(3186..3205)
                     /label="pFastBacR"
                     /note="sequence: caaatgtggtatggctgatt"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1203..1222
                     /label="N58_seqprime2"
                     /note="sequence: caacatcaggtctcggacag"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcccgcgaca tggccacggg gttctccttt gggtccggga ccctcggctc
      721 caccactgtg gcccccggcg gcaccggcac aggcagcgga ttctccttcg gcgcgtccag
      781 cacaccttct gtggggctca acttcggaac tctcggaagc tctgcgacac cagcatctac
      841 atctacttct gccagtggtt ttggaacagg actctttgga tctaagcctg gcactggatt
      901 cactctagga ggaacaagtg caggaacaac agctactaca tctgcatcta caacaggctt
      961 tagtttagga ttcagtaaac ctgcagcatc tgccacacca tttgccctgc ccgtcacttc
     1021 taccacagct agtggtctta ctctctcgtc tgctctgaca tcagctccag cagcatccac
     1081 aggatttacg ttgaataact tgggagcaac accagccaca actacagcag catcaacagg
     1141 actttcctta gggggggcct tagctggttt gggaggttca cttttccaga gtggaaacac
     1201 agcaacatca ggtctcggac agaatgcttt aagcttgtct ctggggactg caacacctac
     1261 ttcagcagcc agcagtgaag gccttggtgg tatagatttt agtacctcat cagataaaaa
     1321 gagtgataaa acgggaacaa ggccagagga tagcaaagcc ctgaaagatg aaaaccttcc
     1381 tcctgtcatc tgtcaggatg ttgagaatct ccagaaattt gtcaaggaac aaaaacaagt
     1441 ccaagaagaa attagtcgaa tgtcttccaa agcaatgctt aaagtccagg aggacatcaa
     1501 ggccctgaag cagctcctgt cattggctgc tagtgggttg cagaggaaca cactcaatat
     1561 tgacaaactg aagttagaaa ctgctcagga gttaaagaat gcggaaatag ctttaagaac
     1621 ccagaaaaca ccacctggac tccaacatga aaatacagct cctgctgact acttcagagt
     1681 cttggttcag caatttgagg tacagcttca gcagtacagg cagcagattg aagaactaga
     1741 aaaccatctt gccactcaag caaacaattc acatataact ccgcaagact tatcaatggc
     1801 tatgcagaaa atttatcaaa catttgtagc tttagctgca caacttcagt ccattcatga
     1861 aaatgtcaag gtgcttaaag aacagtatct cagctacaga aaaatgGAGG AGCTGTTCAC
     1921 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
     1981 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
     2041 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
     2101 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
     2161 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
     2221 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
     2281 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
     2341 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
     2401 CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG
     2461 CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCA AGCTGAGCAA
     2521 AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
     2581 CACTCTCGGC ATGGACGAGC TGTACAAGga tgtgtttgaa gccaggagaa cagaagccaa
     2641 gaagtggcag aatgcaccca gagttactac tggacccacg cctttcagca ccatgccaaa
     2701 cgcagcagcc gttgccatgg ctgcaacact tacacagcag caacagcctg ctacagggcc
     2761 acagccgtct ctgggagtta gttttggaac gccattcggc tcaggtattg gcactggctt
     2821 gcaatcaagt ggcttaggtt cttcaaacct tggaggattt ggaactagct ctggtttcgg
     2881 atgcggtacc acaggggcct ccacatttgg atttggaaca acagataaac cctcaggaag
     2941 tcttagtgca ggctttggca gctcaagtac gtctgggttt aacttcagca atcctggcat
     3001 cacggcatca gctggtttga catttggggt gtccaatcct gcgtctgcgg ggtttggaac
     3061 aggaggacaa ctccttcagt tgaagagacc tcccgctgga aacaaaagag gaaagagatg
     3121 accattgcgc tgtccagagg ttcataaagc tgtgtacaag taaagcggcc gcgactctag
     3181 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3241 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3301 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3361 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     3421 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3481 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3541 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3601 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3661 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3721 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3781 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3841 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3901 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3961 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     4021 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4081 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4141 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4201 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4261 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4321 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     4381 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4441 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4501 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4561 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4621 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4681 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4741 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4801 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4861 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4921 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4981 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     5041 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     5101 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5161 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5221 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5281 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5341 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     5401 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     5461 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5521 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5581 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5641 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5701 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5761 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5821 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5881 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5941 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6001 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6061 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6121 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6181 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6241 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6301 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6361 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6421 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6481 cgtattaccg ccatgcat
//