LOCUS       Nup54-mEGFP494(0)       6306 bp ds-DNA     linear       03-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2162..2176
                     /label="RigidLink5"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     PCR_primer      526..551
                     /label="GGGCGGTAGGCGTGTACGGTGGGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2926
                     /label="Structured region"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     PCR_primer      512..536
                     /label="CCATTGACGCAAATGGGCGGTAGGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2018..2023
                     /label="FG8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        3851..4167
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1715..2161
                     /label="a-helical region"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    938..943
                     /label="FG7"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     PCR_primer      complement(680..699)
                     /label=""
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    878..883
                     /label="FG6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    866..871
                     /label="FG5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    761..766
                     /label="FG3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    860..865
                     /label="FG4"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     PCR_primer      complement(857..882)
                     /label=""
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        3971..4167
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     gene            680..2926
                     /label="Nup54"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      5528..6210
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    752..757
                     /label="FG2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    672..679
                     /label=""
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    692..697
                     /label="FG1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(3354..3660)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             4205..4996
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     PCR_primer      680..699
                     /label="C0070"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     gene            2177..2878
                     /label="mEGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     PCR_primer      complement(2942..2960)
                     /label=""
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2177..2878
                     /label="a-helical region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          1907..1926
                     /label="FG8_seqprime"
                     /note="sequence: gaagaacagttgcgggttca"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcccgcgaca tggcattcaa ttttggggct ccttcgggca cttcgggcac
      721 ctctacagcc accgcggcac cggcgggcgg gtttggcgga tttggaacaa caactacaac
      781 tgcaggctct gcattcagct tttctgcccc aacaaacaca ggcagtacag gccttctcgg
      841 cggcactcag aacaaaggtt ttggctttgg cactggtttt ggcacatcga cgggtactgg
      901 cactggttta ggcactggct tgggaaccgg acttggattc ggaggattta acacccagca
      961 gcagcagcag cagcagcaga cttctttagg cggtctcttc agtcagcctg cacaggcccc
     1021 tgcgcagtcc aaccaactca tcaacactgc cagcgctctt tctgctccaa cgctattggg
     1081 ggatgagaga gatgccatct tggcaaagtg gaaccagttg caggccttct ggggaacagg
     1141 aaaaggatat ttcaataata acattcctcc agtggagttt acacaggaaa accccttttg
     1201 ccgatttaag gctgtcggtt acagttgtat gcccaataac aaagatgaag atggactagt
     1261 ggttctaatt ttcaacaaaa aagaaacaga tattagaagc caacaacagc agttggtaga
     1321 atcattgcat aaagttctgg gaggaaacca gaccctcact gtcaatgtgg agggcattaa
     1381 gacactgccc gacgatcaga cagaagttgt catttacatt gttgagcgct ctccaaatgg
     1441 cacctcgaga agagttccag cgacaacgct gtatgcccac tttgagcaag ccaacattaa
     1501 aacacagctt cagcagcttg gcgtgaccct ttctatgacc agaacagagc tgtctcctgc
     1561 tcagatcaag cagctcctac agaaccctcc tgctggtgtt gatcccatta tttgggaaca
     1621 ggccaaggtg gataacccgg attctgaaaa attaattcct gttccaatgg tgggtttcaa
     1681 agaattgctt cgaagactga aggttcaaga ccagatgact aagcagcacc agaccagatt
     1741 agatatcata tctgaagata ttagtgaact gcaaaagaat caaactacaa ctatggccaa
     1801 aatagcacaa tacaagagga aactgatgga tctctcacac agaaccttac aggtcctaat
     1861 caagcaggaa atccagagga agagtggcta tgccatccag gctgaggaag aacagttgcg
     1921 ggttcagcta gatacgattc aaggagagct gaatgcaccc actcagttca aaggtcgact
     1981 gaatgaactg atgtcccaga tcaggatgca gaatcatttt ggagctgtga aatctgaaga
     2041 aaagtattac atagatgcag acctattacg agagatcaag cagcatttga aacagcaaca
     2101 ggaaggcctt agccacctga ttagcatcat aaaagacgac ctggaagata tcaagctggt
     2161 aGAAGCTGCT GCAAAGGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     2221 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     2281 CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     2341 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA
     2401 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     2461 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     2521 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2581 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA
     2641 CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC
     2701 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
     2761 CTACCTGAGC ACCCAGTCCA AGCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
     2821 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGga
     2881 gcatggactg aatgagacca tccacagcag aggcggggtc tttagctgac cattgcgctg
     2941 tccagaggtt cataaagctg tgtacaagta aagcggccgc gactctagat cataatcagc
     3001 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3061 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3121 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3181 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat
     3241 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3301 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3361 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3421 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3481 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3541 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3601 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3661 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3721 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3781 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3841 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3901 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3961 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4021 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4081 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4141 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4381 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5161 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5221 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5281 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     5341 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5401 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5461 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5521 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6301 atgcat
//