LOCUS Nup54-mEGFP494(1) 6309 bp ds-DNA linear 03-NOV-2020 DEFINITION . FEATURES Location/Qualifiers promoter 3974..4170 /label="SV40 early promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc rep_origin 5531..6213 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 761..766 /label="FG3" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 4208..4999 /label="Kan/neoR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 752..757 /label="FG2" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter 3854..4170 /label="SV40 promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 692..697 /label="FG1" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin complement(3357..3663) /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 gene 680..2929 /label="Nup54" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc PCR_primer complement(2945..2963) /label="" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 PCR_primer 680..699 /label="C0070" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 gene 2180..2881 /label="mEGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 PCR_primer complement(857..882) /label="" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 PCR_primer complement(680..699) /label="" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2180..2881 /label="a-helical region" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 672..679 /label="" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 2162..2179 /label="RigidLink6" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 PCR_primer 526..551 /label="GGGCGGTAGGCGTGTACGGTGGGAGG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2144..2929 /label="Structured region" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 PCR_primer 512..536 /label="CCATTGACGCAAATGGGCGGTAGGC" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2018..2023 /label="FG8" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter 365..568 /label="CMV promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1715..2161 /label="a-helical region" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter 61..364 /label="CMV enhancer" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 938..943 /label="FG7" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 878..883 /label="FG6" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 866..871 /label="FG5" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 860..865 /label="FG4" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer 1907..1926 /label="FG8_seqprime" /note="sequence: gaagaacagttgcgggttca" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer complement(3047..3063) /label="EGFP-C-REV" /note="sequence: gttcagggggaggtgtg" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer 519..539 /label="CMV-FWD" /note="sequence: CGCAAATGGGCGGTAGGCGTG" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(2997..3016) /label="pFastBacR" /note="sequence: caaatgtggtatggctgatt" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcccgcgaca tggcattcaa ttttggggct ccttcgggca cttcgggcac 721 ctctacagcc accgcggcac cggcgggcgg gtttggcgga tttggaacaa caactacaac 781 tgcaggctct gcattcagct tttctgcccc aacaaacaca ggcagtacag gccttctcgg 841 cggcactcag aacaaaggtt ttggctttgg cactggtttt ggcacatcga cgggtactgg 901 cactggttta ggcactggct tgggaaccgg acttggattc ggaggattta acacccagca 961 gcagcagcag cagcagcaga cttctttagg cggtctcttc agtcagcctg cacaggcccc 1021 tgcgcagtcc aaccaactca tcaacactgc cagcgctctt tctgctccaa cgctattggg 1081 ggatgagaga gatgccatct tggcaaagtg gaaccagttg caggccttct ggggaacagg 1141 aaaaggatat ttcaataata acattcctcc agtggagttt acacaggaaa accccttttg 1201 ccgatttaag gctgtcggtt acagttgtat gcccaataac aaagatgaag atggactagt 1261 ggttctaatt ttcaacaaaa aagaaacaga tattagaagc caacaacagc agttggtaga 1321 atcattgcat aaagttctgg gaggaaacca gaccctcact gtcaatgtgg agggcattaa 1381 gacactgccc gacgatcaga cagaagttgt catttacatt gttgagcgct ctccaaatgg 1441 cacctcgaga agagttccag cgacaacgct gtatgcccac tttgagcaag ccaacattaa 1501 aacacagctt cagcagcttg gcgtgaccct ttctatgacc agaacagagc tgtctcctgc 1561 tcagatcaag cagctcctac agaaccctcc tgctggtgtt gatcccatta tttgggaaca 1621 ggccaaggtg gataacccgg attctgaaaa attaattcct gttccaatgg tgggtttcaa 1681 agaattgctt cgaagactga aggttcaaga ccagatgact aagcagcacc agaccagatt 1741 agatatcata tctgaagata ttagtgaact gcaaaagaat caaactacaa ctatggccaa 1801 aatagcacaa tacaagagga aactgatgga tctctcacac agaaccttac aggtcctaat 1861 caagcaggaa atccagagga agagtggcta tgccatccag gctgaggaag aacagttgcg 1921 ggttcagcta gatacgattc aaggagagct gaatgcaccc actcagttca aaggtcgact 1981 gaatgaactg atgtcccaga tcaggatgca gaatcatttt ggagctgtga aatctgaaga 2041 aaagtattac atagatgcag acctattacg agagatcaag cagcatttga aacagcaaca 2101 ggaaggcctt agccacctga ttagcatcat aaaagacgac ctggaagata tcaagctggt 2161 aGCAGAAGCT GCTGCAAAGG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT 2221 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC 2281 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 2341 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT 2401 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT 2461 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC 2521 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG 2581 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA 2641 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT 2701 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA 2761 CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT 2821 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA 2881 Ggagcatgga ctgaatgaga ccatccacag cagaggcggg gtctttagct gaccattgcg 2941 ctgtccagag gttcataaag ctgtgtacaa gtaaagcggc cgcgactcta gatcataatc 3001 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg 3061 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 3121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3181 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa 3241 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc 3301 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt 3361 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa 3421 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg 3481 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg 3541 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc 3601 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa 3661 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 3721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 3781 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat 3841 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 3901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 3961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 4021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 4081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 4141 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt 4201 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 4261 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 4321 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 4381 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 4441 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 4501 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 4561 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 4621 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 4681 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 4741 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 4801 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 4861 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 4921 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 4981 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 5041 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 5101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 5161 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc 5221 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat 5281 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg 5341 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc 5401 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat 5461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 5521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 5761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 6001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 6061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 6121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 6181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 6241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 6301 gccatgcat //