LOCUS       Nup54-mEGFP494(flex1)   6309 bp ds-DNA     linear       03-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2180..2881
                     /label="a-helical region"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    752..757
                     /label="FG2"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2162..2179
                     /label="FlexLink6"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    692..697
                     /label="FG1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2144..2929
                     /label="Structured region"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    672..679
                     /label=""
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            680..2929
                     /label="Nup54"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2018..2023
                     /label="FG8"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     PCR_primer      680..699
                     /label="C0070"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     PCR_primer      512..536
                     /label="CCATTGACGCAAATGGGCGGTAGGC"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1715..2161
                     /label="a-helical region"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        3974..4170
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      5531..6213
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    938..943
                     /label="FG7"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        61..364
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4208..4999
                     /label="Kan/neoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    878..883
                     /label="FG6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        3854..4170
                     /label="SV40 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    866..871
                     /label="FG5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     PCR_primer      complement(680..699)
                     /label=""
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(3357..3663)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    860..865
                     /label="FG4"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     PCR_primer      526..551
                     /label="GGGCGGTAGGCGTGTACGGTGGGAGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     PCR_primer      complement(2945..2963)
                     /label=""
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     PCR_primer      complement(857..882)
                     /label=""
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     gene            2180..2881
                     /label="mEGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        365..568
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    761..766
                     /label="FG3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2997..3016)
                     /label="pFastBacR"
                     /note="sequence: caaatgtggtatggctgatt"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1212..1231
                     /label="seqPrime_N54_1_new"
                     /note="sequence: ctgtcggttacagttgtatg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccaccgg tcccgcgaca tggcattcaa ttttggggct ccttcgggca cttcgggcac
      721 ctctacagcc accgcggcac cggcgggcgg gtttggcgga tttggaacaa caactacaac
      781 tgcaggctct gcattcagct tttctgcccc aacaaacaca ggcagtacag gccttctcgg
      841 cggcactcag aacaaaggtt ttggctttgg cactggtttt ggcacatcga cgggtactgg
      901 cactggttta ggcactggct tgggaaccgg acttggattc ggaggattta acacccagca
      961 gcagcagcag cagcagcaga cttctttagg cggtctcttc agtcagcctg cacaggcccc
     1021 tgcgcagtcc aaccaactca tcaacactgc cagcgctctt tctgctccaa cgctattggg
     1081 ggatgagaga gatgccatct tggcaaagtg gaaccagttg caggccttct ggggaacagg
     1141 aaaaggatat ttcaataata acattcctcc agtggagttt acacaggaaa accccttttg
     1201 ccgatttaag gctgtcggtt acagttgtat gcccaataac aaagatgaag atggactagt
     1261 ggttctaatt ttcaacaaaa aagaaacaga tattagaagc caacaacagc agttggtaga
     1321 atcattgcat aaagttctgg gaggaaacca gaccctcact gtcaatgtgg agggcattaa
     1381 gacactgccc gacgatcaga cagaagttgt catttacatt gttgagcgct ctccaaatgg
     1441 cacctcgaga agagttccag cgacaacgct gtatgcccac tttgagcaag ccaacattaa
     1501 aacacagctt cagcagcttg gcgtgaccct ttctatgacc agaacagagc tgtctcctgc
     1561 tcagatcaag cagctcctac agaaccctcc tgctggtgtt gatcccatta tttgggaaca
     1621 ggccaaggtg gataacccgg attctgaaaa attaattcct gttccaatgg tgggtttcaa
     1681 agaattgctt cgaagactga aggttcaaga ccagatgact aagcagcacc agaccagatt
     1741 agatatcata tctgaagata ttagtgaact gcaaaagaat caaactacaa ctatggccaa
     1801 aatagcacaa tacaagagga aactgatgga tctctcacac agaaccttac aggtcctaat
     1861 caagcaggaa atccagagga agagtggcta tgccatccag gctgaggaag aacagttgcg
     1921 ggttcagcta gatacgattc aaggagagct gaatgcaccc actcagttca aaggtcgact
     1981 gaatgaactg atgtcccaga tcaggatgca gaatcatttt ggagctgtga aatctgaaga
     2041 aaagtattac atagatgcag acctattacg agagatcaag cagcatttga aacagcaaca
     2101 ggaaggcctt agccacctga ttagcatcat aaaagacgac ctggaagata tcaagctggt
     2161 aGGGGGTGGC GGCAGCTCCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT
     2221 GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC
     2281 CTACGGCAAG CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC
     2341 CACCCTCGTG ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT
     2401 GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT
     2461 CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC
     2521 CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG
     2581 GCACAAGCTG GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA
     2641 GAACGGCATC AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT
     2701 CGCCGACCAC TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA
     2761 CCACTACCTG AGCACCCAGT CCAAGCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT
     2821 GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA
     2881 Ggagcatgga ctgaatgaga ccatccacag cagaggcggg gtctttagct gaccattgcg
     2941 ctgtccagag gttcataaag ctgtgtacaa gtaaagcggc cgcgactcta gatcataatc
     3001 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3061 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3121 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3181 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     3241 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3301 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3361 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3421 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3481 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3541 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3601 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3661 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3721 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3781 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     3841 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3901 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3961 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4021 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4081 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4141 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     4201 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4261 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4321 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4381 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4441 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4501 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4561 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4621 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4681 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4741 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4801 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4861 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4921 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4981 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     5041 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5161 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     5221 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
     5281 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
     5341 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
     5401 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
     5461 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5521 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6301 gccatgcat
//