LOCUS Nup54_mEGFP494(-6) 6288 bp ds-DNA linear 04-SEP-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 672..679 /label="" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e rep_origin complement(3336..3642) /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 866..871 /label="FG5" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e PCR_primer 680..699 /label="C0070" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 promoter 61..364 /label="CMV enhancer" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 PCR_primer complement(2924..2942) /label="" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 PCR_primer complement(680..699) /label="" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 CDS 4187..4978 /label="Kan/neoR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2162..2860 /label="a-helical region" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 938..943 /label="FG7" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 692..697 /label="FG1" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1715..2161 /label="a-helical region" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 2018..2023 /label="FG8" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 PCR_primer 512..536 /label="CCATTGACGCAAATGGGCGGTAGGC" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 PCR_primer 526..551 /label="GGGCGGTAGGCGTGTACGGTGGGAGG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 761..766 /label="FG3" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc promoter 3953..4149 /label="SV40 early promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc promoter 365..568 /label="CMV promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 gene 680..2908 /label="Nup54" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc PCR_primer complement(857..882) /label="" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2144..2908 /label="Structured region" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 752..757 /label="FG2" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 gene 2162..2860 /label="mEGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 rep_origin 5510..6192 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 878..883 /label="FG6" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 860..865 /label="FG4" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter 3833..4149 /label="SV40 promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcccgcgaca tggcattcaa ttttggggct ccttcgggca cttcgggcac 721 ctctacagcc accgcggcac cggcgggcgg gtttggcgga tttggaacaa caactacaac 781 tgcaggctct gcattcagct tttctgcccc aacaaacaca ggcagtacag gccttctcgg 841 cggcactcag aacaaaggtt ttggctttgg cactggtttt ggcacatcga cgggtactgg 901 cactggttta ggcactggct tgggaaccgg acttggattc ggaggattta acacccagca 961 gcagcagcag cagcagcaga cttctttagg cggtctcttc agtcagcctg cacaggcccc 1021 tgcgcagtcc aaccaactca tcaacactgc cagcgctctt tctgctccaa cgctattggg 1081 ggatgagaga gatgccatct tggcaaagtg gaaccagttg caggccttct ggggaacagg 1141 aaaaggatat ttcaataata acattcctcc agtggagttt acacaggaaa accccttttg 1201 ccgatttaag gctgtcggtt acagttgtat gcccaataac aaagatgaag atggactagt 1261 ggttctaatt ttcaacaaaa aagaaacaga tattagaagc caacaacagc agttggtaga 1321 atcattgcat aaagttctgg gaggaaacca gaccctcact gtcaatgtgg agggcattaa 1381 gacactgccc gacgatcaga cagaagttgt catttacatt gttgagcgct ctccaaatgg 1441 cacctcgaga agagttccag cgacaacgct gtatgcccac tttgagcaag ccaacattaa 1501 aacacagctt cagcagcttg gcgtgaccct ttctatgacc agaacagagc tgtctcctgc 1561 tcagatcaag cagctcctac agaaccctcc tgctggtgtt gatcccatta tttgggaaca 1621 ggccaaggtg gataacccgg attctgaaaa attaattcct gttccaatgg tgggtttcaa 1681 agaattgctt cgaagactga aggttcaaga ccagatgact aagcagcacc agaccagatt 1741 agatatcata tctgaagata ttagtgaact gcaaaagaat caaactacaa ctatggccaa 1801 aatagcacaa tacaagagga aactgatgga tctctcacac agaaccttac aggtcctaat 1861 caagcaggaa atccagagga agagtggcta tgccatccag gctgaggaag aacagttgcg 1921 ggttcagcta gatacgattc aaggagagct gaatgcaccc actcagttca aaggtcgact 1981 gaatgaactg atgtcccaga tcaggatgca gaatcatttt ggagctgtga aatctgaaga 2041 aaagtattac atagatgcag acctattacg agagatcaag cagcatttga aacagcaaca 2101 ggaaggcctt agccacctga ttagcatcat aaaagacgac ctggaagata tcaagctggt 2161 aGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA 2221 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA 2281 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC 2341 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA 2401 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA 2461 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT 2521 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA 2581 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT 2641 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA 2701 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC 2761 CAAGCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC 2821 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG gagcatggac tgaatgagac 2881 catccacagc agaggcgggg tctttagctg accattgcgc tgtccagagg ttcataaagc 2941 tgtgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag 3001 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 3061 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 3121 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 3181 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 3241 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 3301 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 3361 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 3421 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 3481 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 3541 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 3601 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 3661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 3721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 3781 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 3841 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 3901 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 3961 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 4021 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 4081 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 4141 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 4201 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4261 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4321 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 4381 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4441 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4501 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4561 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4621 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4681 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 4741 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4801 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4861 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4921 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4981 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 5041 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 5101 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 5161 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 5221 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 5281 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 5341 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 5401 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 5461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 5521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 5761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 6121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 6181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 6241 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat //