LOCUS Nup54_mEGFP510(-5) 6291 bp ds-DNA linear 04-SEP-2020 DEFINITION . FEATURES Location/Qualifiers promoter 3836..4152 /label="SV40 promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 860..865 /label="FG4" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 rep_origin complement(3339..3645) /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 PCR_primer complement(857..882) /label="" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 PCR_primer complement(2927..2945) /label="" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 PCR_primer 512..536 /label="CCATTGACGCAAATGGGCGGTAGGC" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 761..766 /label="FG3" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gene 2210..2911 /label="mEGFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 752..757 /label="FG2" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 promoter 61..364 /label="CMV enhancer" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2210..2911 /label="a-helical region" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 692..697 /label="FG1" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 PCR_primer 526..551 /label="GGGCGGTAGGCGTGTACGGTGGGAGG" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2144..2209 /label="Structured region" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 gene 680..2911 /label="Nup54" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2018..2023 /label="FG8" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 PCR_primer 680..699 /label="C0070" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1715..2161 /label="a-helical region" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 PCR_primer complement(680..699) /label="" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 938..943 /label="FG7" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 promoter 365..568 /label="CMV promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter 3956..4152 /label="SV40 early promoter" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc rep_origin 5513..6195 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 878..883 /label="FG6" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS 4190..4981 /label="Kan/neoR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 672..679 /label="" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 866..871 /label="FG5" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(2979..2998) /label="pFastBacR" /note="sequence: caaatgtggtatggctgatt" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatccaccgg tcccgcgaca tggcattcaa ttttggggct ccttcgggca cttcgggcac 721 ctctacagcc accgcggcac cggcgggcgg gtttggcgga tttggaacaa caactacaac 781 tgcaggctct gcattcagct tttctgcccc aacaaacaca ggcagtacag gccttctcgg 841 cggcactcag aacaaaggtt ttggctttgg cactggtttt ggcacatcga cgggtactgg 901 cactggttta ggcactggct tgggaaccgg acttggattc ggaggattta acacccagca 961 gcagcagcag cagcagcaga cttctttagg cggtctcttc agtcagcctg cacaggcccc 1021 tgcgcagtcc aaccaactca tcaacactgc cagcgctctt tctgctccaa cgctattggg 1081 ggatgagaga gatgccatct tggcaaagtg gaaccagttg caggccttct ggggaacagg 1141 aaaaggatat ttcaataata acattcctcc agtggagttt acacaggaaa accccttttg 1201 ccgatttaag gctgtcggtt acagttgtat gcccaataac aaagatgaag atggactagt 1261 ggttctaatt ttcaacaaaa aagaaacaga tattagaagc caacaacagc agttggtaga 1321 atcattgcat aaagttctgg gaggaaacca gaccctcact gtcaatgtgg agggcattaa 1381 gacactgccc gacgatcaga cagaagttgt catttacatt gttgagcgct ctccaaatgg 1441 cacctcgaga agagttccag cgacaacgct gtatgcccac tttgagcaag ccaacattaa 1501 aacacagctt cagcagcttg gcgtgaccct ttctatgacc agaacagagc tgtctcctgc 1561 tcagatcaag cagctcctac agaaccctcc tgctggtgtt gatcccatta tttgggaaca 1621 ggccaaggtg gataacccgg attctgaaaa attaattcct gttccaatgg tgggtttcaa 1681 agaattgctt cgaagactga aggttcaaga ccagatgact aagcagcacc agaccagatt 1741 agatatcata tctgaagata ttagtgaact gcaaaagaat caaactacaa ctatggccaa 1801 aatagcacaa tacaagagga aactgatgga tctctcacac agaaccttac aggtcctaat 1861 caagcaggaa atccagagga agagtggcta tgccatccag gctgaggaag aacagttgcg 1921 ggttcagcta gatacgattc aaggagagct gaatgcaccc actcagttca aaggtcgact 1981 gaatgaactg atgtcccaga tcaggatgca gaatcatttt ggagctgtga aatctgaaga 2041 aaagtattac atagatgcag acctattacg agagatcaag cagcatttga aacagcaaca 2101 ggaaggcctt agccacctga ttagcatcat aaaagacgac ctggaagata tcaagctggt 2161 agagcatgga ctgaatgaga ccatccacag cagaggcggg gtctttagcG AGGAGCTGTT 2221 CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG 2281 CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGTTCATCTG 2341 CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCCTGA CCTACGGCGT 2401 GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT 2461 GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC 2521 CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT 2581 CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG GAGTACAACT ACAACAGCCA 2641 CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC AAGGTGAACT TCAAGATCCG 2701 CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC TACCAGCAGA ACACCCCCAT 2761 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCACCCAGT CCAAGCTGAG 2821 CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG 2881 GATCACTCTC GGCATGGACG AGCTGTACAA Gtgaccattg cgctgtccag aggttcataa 2941 agctgtgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta 3001 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 3061 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 3121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 3181 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc 3241 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 3301 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 3361 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 3421 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 3481 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 3541 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 3601 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 3661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 3721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 3781 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 3841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 3901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 4021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 4081 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 4141 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 4201 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4261 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4321 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 4381 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4441 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4501 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4561 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4621 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4681 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 4741 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4801 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 4861 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 4921 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 4981 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 5041 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 5101 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 5161 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 5221 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 5281 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 5341 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 5401 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 5461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 5521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 5581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 5641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 5701 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 5761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 5821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 5881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 5941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 6001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 6061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 6121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 6181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 6241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t //