LOCUS pET28-RAN 5944 bp ds-DNA circular 03-NOV-2020 DEFINITION . FEATURES Location/Qualifiers misc_feature 2677..3324 /label="RAN" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_binding 2549..2571 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2629..2646 /label="6xHis" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac rep_origin 3519..3825 /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 rep_origin 3502..3957 /label="M13 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 3334..3351 /label="6x His Tag" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS complement(4053..4865) /label="KanR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin 4911..5593 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 2530..2549 /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(3404..3422) /label="T7-term" /note="sequence: gctagttattgctcagcgg" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc ORIGIN 1 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 61 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc 121 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt 181 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc 241 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc 301 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact 361 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac 421 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca 481 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag 541 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt 601 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag 661 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg 721 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 781 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 841 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 901 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 961 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 1021 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 1081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 1141 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 1201 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 1261 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 1321 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 1381 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 1441 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 1501 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 1561 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 1621 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 1681 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 1741 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 1801 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 1861 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 1921 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 1981 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 2041 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 2101 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 2161 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 2221 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 2281 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 2341 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 2401 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 2461 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 2521 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 2581 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat 2641 catcacagca gcggcctggt gccgcgcggc agccatatgg ctGCGCAGGG AGAGCCCCAG 2701 GTCCAGTTCA AACTTGTATT GGTTGGTGAT GGTGGTACTG GAAAAACGAC CTTCGTGAAA 2761 CGTCATTTGA CTGGTGAATT TGAGAAGAAG TATGTAGCCA CCTTGGGTGT TGAGGTTCAT 2821 CCCCTAGTGT TCCACACCAA CAGAGGACCT ATTAAGTTCA ATGTATGGGA CACAGCCGGC 2881 CAGGAGAAAT TCGGTGGACT GAGAGATGGC TATTATATCC AAGCCCAGTG TGCCATCATA 2941 ATGTTTGATG TAACATCGAG AGTTACTTAC AAGAATGTGC CTAACTGGCA TAGAGATCTG 3001 GTACGAGTGT GTGAAAACAT CCCCATTGTG TTGTGTGGCA ACAAAGTGGA TATTAAGGAC 3061 AGGAAAGTGA AGGCGAAATC CATTGTCTTC CACCGAAAGA AGAATCTTCA GTACTACGAC 3121 ATTTCTGCCA AAAGTAACTA CAACTTTGAA AAGCCCTTCC TCTGGCTTGC TAGGAAGCTC 3181 ATTGGAGACC CTAACTTGGA ATTTGTTGCC ATGCCTGCTC TCGCCCCACC AGAAGTTGTC 3241 ATGGACCCAG CTTTGGCAGC ACAGTATGAG CACGACTTAG AGGTTGCTCA GACAACTGCT 3301 CTCCCGGATG AGGATGATGA CCTGTGActc gagcaccacc accaccacca ctgagatccg 3361 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta 3421 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact 3481 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 3541 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 3601 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 3661 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 3721 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 3781 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 3841 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 3901 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag 3961 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 4021 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac 4081 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat 4141 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg 4201 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta 4261 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc 4321 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca 4381 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg 4441 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca 4501 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg 4561 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc 4621 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg 4681 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat 4741 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa 4801 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg 4861 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa 4921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg 4981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc 5041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac 5101 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca 5161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt 5221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc 5281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 5341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc 5401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 5461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct 5521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 5581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt 5641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac 5701 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg 5761 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc 5821 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc 5881 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac 5941 gggc //