LOCUS pET28-KPNB1 7489 bp ds-DNA circular 03-NOV-2020 DEFINITION . FEATURES Location/Qualifiers primer_bind 2530..2549 /label="T7" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2629..2646 /label="6xHis" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac rep_origin 5047..5502 /label="M13 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2677..4872 /label="KAP1B" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 4879..4896 /label="6x His Tag" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc rep_origin 6456..7138 /label="ColE1 origin" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(5598..6410) /label="KanR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 rep_origin 5064..5370 /label="F1 ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_binding 2549..2571 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer complement(4949..4967) /label="T7-term" /note="sequence: gctagttattgctcagcgg" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 3165..3185 /label="KPNB1_Seq1" /note="sequence: GCACACCAGCAAGTTTTATGC" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(2662..2676) /label="Rev_AmpPET28forNTF2_2" /note="sequence: atggctgccgcgcgg" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer 4873..4893 /label="FOR_AmpPET28forNTF2_2" /note="sequence: ctcgagcaccaccaccaccac" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer 3768..3790 /label="KPNB1_Seq2" /note="sequence: GCTCCTAGAGACTACAGACAGAC" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 2903..2922 /label="KPNB1_Seq3" /note="sequence: TGGTCTGTGAAGCCACACAG" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 61 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc 121 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt 181 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc 241 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc 301 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact 361 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac 421 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca 481 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag 541 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt 601 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag 661 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg 721 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 781 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 841 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 901 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 961 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 1021 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 1081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 1141 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 1201 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 1261 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 1321 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 1381 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 1441 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 1501 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 1561 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 1621 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 1681 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 1741 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 1801 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 1861 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 1921 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 1981 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 2041 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 2101 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 2161 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 2221 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 2281 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac 2341 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg 2401 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca 2461 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc 2521 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 2581 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat 2641 catcacagca gcggcctggt gccgcgcggc agccatATGA AGGAGTCGAC ATTGGAAGCC 2701 ATCGGTTATA TTTGCCAAGA TATAGACCCA GAGCAGCTAC AAGATAAATC CAATGAGATT 2761 CTGACTGCCA TAATCCAGGG GATGAGGAAA GAAGAGCCTA GTAATAATGT GAAGCTAGCT 2821 GCTACGAATG CACTCCTGAA CTCATTGGAG TTCACCAAAG CAAACTTTGA TAAAGAGTCT 2881 GAAAGGCACT TTATTATGCA GGTGGTCTGT GAAGCCACAC AGTGTCCAGA TACGAGGGTA 2941 CGAGTGGCTG CTTTACAGAA TCTGGTGAAG ATAATGTCCT TATATTATCA GTACATGGAG 3001 ACATATATGG GTCCTGCTCT TTTTGCAATC ACAATCGAAG CAATGAAAAG TGACATTGAT 3061 GAGGTGGCTT TACAAGGGAT AGAATTCTGG TCCAATGTCT GTGATGAGGA AATGGATTTG 3121 GCCATTGAAG CTTCAGAGGC AGCAGAACAA GGACGGCCCC CTGAGCACAC CAGCAAGTTT 3181 TATGCGAAGG GAGCACTACA GTATCTGGTT CCAATCCTCA CACAGACACT AACTAAACAG 3241 GACGAAAATG ATGATGACGA TGACTGGAAC CCCTGCAAAG CAGCAGGGGT GTGCCTCATG 3301 CTTCTGGCCA CCTGCTGTGA AGATGACATT GTCCCACATG TCCTCCCCTT CATTAAAGAA 3361 CACATCAAGA ACCCAGATTG GCGGTACCGG GATGCAGCAG TGATGGCTTT TGGTTGTATC 3421 TTGGAAGGAC CAGAGCCCAG TCAGCTCAAA CCACTAGTTA TACAGGCTAT GCCCACCCTA 3481 ATAGAATTAA TGAAAGACCC CAGTGTAGTT GTTCGAGATA CAGCTGCATG GACTGTAGGC 3541 AGAATTTGTG AGCTGCTTCC TGAAGCTGCC ATCAATGATG TCTACTTGGC TCCCCTGCTA 3601 CAGTGTCTGA TTGAGGGTCT CAGTGCTGAA CCCAGAGTGG CTTCAAATGT GTGCTGGGCT 3661 TTCTCCAGTC TGGCTGAAGC TGCTTATGAA GCTGCAGACG TTGCTGATGA TCAGGAAGAA 3721 CCAGCTACTT ACTGCTTATC TTCTTCATTT GAACTCATAG TTCAGAAGCT CCTAGAGACT 3781 ACAGACAGAC CTGATGGACA CCAGAACAAC CTGAGGAGTT CTGCATATGA ATCTCTGATG 3841 GAAATTGTGA AAAACAGTGC CAAGGATTGT TATCCTGCTG TCCAGAAAAC GACTTTGGTC 3901 ATCATGGAAC GACTGCAACA GGTTCTTCAG ATGGAGTCAC ATATCCAGAG CACATCCGAT 3961 AGAATCCAGT TCAATGACCT TCAGTCTTTA CTCTGTGCAA CTCTTCAGAA TGTTCTTCGG 4021 AAAGTGCAAC ATCAAGATGC TTTGCAGATC TCTGATGTGG TTATGGCCTC CCTGTTAAGG 4081 ATGTTCCAAA GCACAGCTGG GTCTGGGGGA GTACAAGAGG ATGCCCTGAT GGCAGTTAGC 4141 ACACTGGTGG AAGTGTTGGG TGGTGAATTC CTCAAGTACA TGGAGGCCTT TAAACCCTTC 4201 CTGGGCATTG GATTAAAAAA TTATGCTGAA TACCAGGTTT GTTTGGCAGC TGTGGGCTTA 4261 GTGGGAGACT TGTGCCGTGC CCTGCAATCC AACATCATAC CTTTCTGTGA CGAGGTGATG 4321 CAGCTGCTTC TGGAAAATTT GGGGAATGAG AACGTCCACA GGTCTGTGAA GCCGCAGATT 4381 CTGTCAGTGT TTGGTGATAT TGCCCTTGCT ATTGGAGGAG AGTTTAAAAA ATACTTAGAG 4441 GTTGTATTGA ATACTCTTCA GCAGGCCTCC CAAGCCCAGG TGGACAAGTC AGACTATGAC 4501 ATGGTGGATT ATCTGAATGA GCTAAGGGAA AGCTGCTTGG AAGCCTATAC TGGAATCGTC 4561 CAGGGATTAA AGGGGGATCA GGAGAACGTA CACCCGGATG TGATGCTGGT ACAACCCAGA 4621 GTAGAATTTA TTCTGTCTTT CATTGACCAC ATTGCTGGAG ATGAGGATCA CACAGATGGA 4681 GTAGTAGCTT GTGCTGCTGG ACTAATAGGG GACTTATGTA CAGCATTTGG GAAGGATGTA 4741 CTGAAATTAG TAGAAGCTAG GCCAATGATC CATGAATTGT TAACTGAAGG GCGGAGATCG 4801 AAGACTAACA AAGCAAAAAC CCTTGCTACA TGGGCAACAA AAGAACTGAG GAAACTGAAG 4861 AACCAAGCTT GActcgagca ccaccaccac caccactgag atccggctgc taacaaagcc 4921 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg 4981 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg 5041 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 5101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 5161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 5221 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 5281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 5341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 5401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 5461 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg 5521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 5581 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata 5641 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca 5701 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca 5761 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca 5821 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact 5881 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta 5941 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta 6001 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca 6061 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg 6121 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat 6181 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg 6241 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca 6301 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg 6361 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt 6421 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga 6481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 6541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 6601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 6661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc 6721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 6781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 6841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 6901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 6961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 7021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 7081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 7141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 7201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 7261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt 7321 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc 7381 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 7441 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggc //