LOCUS       pET28-KPNB1             7489 bp ds-DNA     circular     03-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     2530..2549
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2629..2646
                     /label="6xHis"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      5047..5502
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2677..4872
                     /label="KAP1B"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4879..4896
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      6456..7138
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(5598..6410)
                     /label="KanR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      5064..5370
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_binding    2549..2571
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4949..4967)
                     /label="T7-term"
                     /note="sequence: gctagttattgctcagcgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          3165..3185
                     /label="KPNB1_Seq1"
                     /note="sequence: GCACACCAGCAAGTTTTATGC"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2662..2676)
                     /label="Rev_AmpPET28forNTF2_2"
                     /note="sequence: atggctgccgcgcgg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4873..4893
                     /label="FOR_AmpPET28forNTF2_2"
                     /note="sequence: ctcgagcaccaccaccaccac"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3768..3790
                     /label="KPNB1_Seq2"
                     /note="sequence: GCTCCTAGAGACTACAGACAGAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2903..2922
                     /label="KPNB1_Seq3"
                     /note="sequence: TGGTCTGTGAAGCCACACAG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
        1 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
       61 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
      121 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
      181 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
      241 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc
      301 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact
      361 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac
      421 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca
      481 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag
      541 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt
      601 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag
      661 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg
      721 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
      781 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
      841 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
      901 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
      961 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     1021 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1081 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1141 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     1201 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     1261 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     1321 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     1381 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     1441 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     1501 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     1561 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     1621 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     1681 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     1741 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     1801 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     1861 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     1921 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     1981 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     2041 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     2101 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     2161 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     2221 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     2281 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     2341 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     2401 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     2461 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     2521 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     2581 aaataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat
     2641 catcacagca gcggcctggt gccgcgcggc agccatATGA AGGAGTCGAC ATTGGAAGCC
     2701 ATCGGTTATA TTTGCCAAGA TATAGACCCA GAGCAGCTAC AAGATAAATC CAATGAGATT
     2761 CTGACTGCCA TAATCCAGGG GATGAGGAAA GAAGAGCCTA GTAATAATGT GAAGCTAGCT
     2821 GCTACGAATG CACTCCTGAA CTCATTGGAG TTCACCAAAG CAAACTTTGA TAAAGAGTCT
     2881 GAAAGGCACT TTATTATGCA GGTGGTCTGT GAAGCCACAC AGTGTCCAGA TACGAGGGTA
     2941 CGAGTGGCTG CTTTACAGAA TCTGGTGAAG ATAATGTCCT TATATTATCA GTACATGGAG
     3001 ACATATATGG GTCCTGCTCT TTTTGCAATC ACAATCGAAG CAATGAAAAG TGACATTGAT
     3061 GAGGTGGCTT TACAAGGGAT AGAATTCTGG TCCAATGTCT GTGATGAGGA AATGGATTTG
     3121 GCCATTGAAG CTTCAGAGGC AGCAGAACAA GGACGGCCCC CTGAGCACAC CAGCAAGTTT
     3181 TATGCGAAGG GAGCACTACA GTATCTGGTT CCAATCCTCA CACAGACACT AACTAAACAG
     3241 GACGAAAATG ATGATGACGA TGACTGGAAC CCCTGCAAAG CAGCAGGGGT GTGCCTCATG
     3301 CTTCTGGCCA CCTGCTGTGA AGATGACATT GTCCCACATG TCCTCCCCTT CATTAAAGAA
     3361 CACATCAAGA ACCCAGATTG GCGGTACCGG GATGCAGCAG TGATGGCTTT TGGTTGTATC
     3421 TTGGAAGGAC CAGAGCCCAG TCAGCTCAAA CCACTAGTTA TACAGGCTAT GCCCACCCTA
     3481 ATAGAATTAA TGAAAGACCC CAGTGTAGTT GTTCGAGATA CAGCTGCATG GACTGTAGGC
     3541 AGAATTTGTG AGCTGCTTCC TGAAGCTGCC ATCAATGATG TCTACTTGGC TCCCCTGCTA
     3601 CAGTGTCTGA TTGAGGGTCT CAGTGCTGAA CCCAGAGTGG CTTCAAATGT GTGCTGGGCT
     3661 TTCTCCAGTC TGGCTGAAGC TGCTTATGAA GCTGCAGACG TTGCTGATGA TCAGGAAGAA
     3721 CCAGCTACTT ACTGCTTATC TTCTTCATTT GAACTCATAG TTCAGAAGCT CCTAGAGACT
     3781 ACAGACAGAC CTGATGGACA CCAGAACAAC CTGAGGAGTT CTGCATATGA ATCTCTGATG
     3841 GAAATTGTGA AAAACAGTGC CAAGGATTGT TATCCTGCTG TCCAGAAAAC GACTTTGGTC
     3901 ATCATGGAAC GACTGCAACA GGTTCTTCAG ATGGAGTCAC ATATCCAGAG CACATCCGAT
     3961 AGAATCCAGT TCAATGACCT TCAGTCTTTA CTCTGTGCAA CTCTTCAGAA TGTTCTTCGG
     4021 AAAGTGCAAC ATCAAGATGC TTTGCAGATC TCTGATGTGG TTATGGCCTC CCTGTTAAGG
     4081 ATGTTCCAAA GCACAGCTGG GTCTGGGGGA GTACAAGAGG ATGCCCTGAT GGCAGTTAGC
     4141 ACACTGGTGG AAGTGTTGGG TGGTGAATTC CTCAAGTACA TGGAGGCCTT TAAACCCTTC
     4201 CTGGGCATTG GATTAAAAAA TTATGCTGAA TACCAGGTTT GTTTGGCAGC TGTGGGCTTA
     4261 GTGGGAGACT TGTGCCGTGC CCTGCAATCC AACATCATAC CTTTCTGTGA CGAGGTGATG
     4321 CAGCTGCTTC TGGAAAATTT GGGGAATGAG AACGTCCACA GGTCTGTGAA GCCGCAGATT
     4381 CTGTCAGTGT TTGGTGATAT TGCCCTTGCT ATTGGAGGAG AGTTTAAAAA ATACTTAGAG
     4441 GTTGTATTGA ATACTCTTCA GCAGGCCTCC CAAGCCCAGG TGGACAAGTC AGACTATGAC
     4501 ATGGTGGATT ATCTGAATGA GCTAAGGGAA AGCTGCTTGG AAGCCTATAC TGGAATCGTC
     4561 CAGGGATTAA AGGGGGATCA GGAGAACGTA CACCCGGATG TGATGCTGGT ACAACCCAGA
     4621 GTAGAATTTA TTCTGTCTTT CATTGACCAC ATTGCTGGAG ATGAGGATCA CACAGATGGA
     4681 GTAGTAGCTT GTGCTGCTGG ACTAATAGGG GACTTATGTA CAGCATTTGG GAAGGATGTA
     4741 CTGAAATTAG TAGAAGCTAG GCCAATGATC CATGAATTGT TAACTGAAGG GCGGAGATCG
     4801 AAGACTAACA AAGCAAAAAC CCTTGCTACA TGGGCAACAA AAGAACTGAG GAAACTGAAG
     4861 AACCAAGCTT GActcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
     4921 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     4981 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     5041 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5101 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5161 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5221 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5281 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5341 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5401 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5461 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg
     5521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5581 tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     5641 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     5701 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     5761 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     5821 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     5881 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     5941 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     6001 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     6061 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     6121 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     6181 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     6241 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     6301 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     6361 gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     6421 gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga
     6481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6661 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6781 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6841 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6901 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6961 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7021 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7081 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7141 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7201 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7261 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
     7321 tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc
     7381 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     7441 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggc
//