LOCUS       Exported                8403 bp ds-DNA     circular UNA 30-OCT-2020
DEFINITION  bpNLS - SapI x2 - BE4max.
ACCESSION   urn.local...361-cl9tw0t
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     198..211
                     /label=ACK15
     primer_bind     198..211
                     /label=JLseq007_ACK15
     primer_bind     234..253
                     /label=LCv2_seq1
     primer_bind     261..285
                     /label=ACK15
     primer_bind     261..285
                     /label=JLseq007_ACK15
     promoter        275..356
                     /label=CMV prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=CMV prom"
                     /note="/Transferred_Similarity=97.56%"
     primer_bind     286..306
                     /label=Genewiz CMV-Forward
     primer_bind     286..306
                     /label=Eton CMV-Forward
     primer_bind     286..306
                     /label=QB0012 CMV Forward
     primer_bind     293..314
                     /label=QB0976 pRLuc-MCS-F
     primer_bind     complement(331..360)
                     /label=352_CMV_Rev2
     primer_bind     377..396
                     /label=T7
     primer_bind     377..396
                     /label=Genewiz T7
     primer_bind     377..396
                     /label=Eton T7 Promoter
     primer_bind     377..396
                     /label=QB0106 T7
     primer_bind     377..396
                     /label=T7 promoter
     promoter        377..395
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(382..408)
                     /label=197_pCMV_bb_F
     primer_bind     complement(385..408)
                     /label=203_bb_NpuC_R
     primer_bind     complement(389..447)
                     /label=875_Genscript_APOBEC-bpnls-rev
     primer_bind     389..428
                     /label=903_GS_NpuN-fwd
     primer_bind     complement(389..408)
                     
     primer_bind     complement(389..408)
                     
     misc_feature    406..408
                     /label=USER1
                     /note="/created_by=User"
     CDS             409..465
                     /label=NLS
                     /note="/created_by=User"
     misc_feature    466..1149
                     /label=Anc689
                     /note="/created_by=lkoblan"
     misc_feature    466..1128
                     /label=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCT
                     /label=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCT 
                     TAGGATGGGACTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATT 
                     AACAAAATTATAATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGA 
                     AAGAAAAGCAAGTCAACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCAC 
                     CAGCCAACAGCTAAGAAAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAA 
                     CAAAGCCGAAATTTGAGCAATACCATAGTGCACATTGACATGGATGCTTTCTATGCAG 
                     CTGTAGAAATGAGGGACAATCCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAAT 
                     GAGTATGCTGTCTACTTCAAATTACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATG 
                     CCAGGATTTATTGCTAAGAGGCTGTGCCCACAACTTATAATAGTGCCCCCCAACTTTG 
                     ACAAATACCGAGCTGTGAGTAAAGAGGTTAAGGAAATACTTGCTGATTATGATCCCAA 
                     TTTTATGGCCATGAGTCTTGATGAAGCCTACTTGAATATAACAAAGCACTTAGAAGAA 
                     AGACAAAATTGGCCTGAGGATAAAAGAAGGTATTTCATCAAAATGGGAAGCTCTGTAG 
                     AAAATGATAATCCAGGAAAGGAAGTTAATAAACTGAGTGAGCATGAACGATCCATCTC 
                     TCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGCAGCCCCCAGGAGATCCTTTCCAA 
                     GTGAACTTTGAAGAACAAAACAATCCTCAAATACTCCAAAACTCAGTTGTTTTTGGAA 
                     CATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGAATTGAGCAGAAAACAACACT 
                     GACAGCCAGTGCAGGCATTGCCCCAAATACAATGTTAGCAAAAGTATGCAGTGATAAG 
                     AATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAAGCTGTGATGGACTTCA 
                     TCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTTACAGAGAAAATGTT 
                     AAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGGGCATTGCTTTCT 
                     CTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTGGGTCTAGGTT 
                     CAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAGGACATTCAG 
                     TGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGCAGTGAGCTT 
                     GCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTAAGTTGAAGA 
                     ATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGTTTCTACTGC 
                     AGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGATGCTGATTTT 
                     CCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTCCCAATGAAG 
                     AGGACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGGAAACCAAGC 
                     CCTGTCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTTGTAAAACCT 
                     CTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAAGGAAATGGA 
                     GTCACCAAGATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCAGACATCACA 
                     ACCATTCCAAGTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCAGAGAATTCA 
                     GATGACTGTCAGATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGTGCATCAGTC 
                     TGGAAGCCTTGAATAAACATGTAGATGAATGTCTTGATGGACCTTCAATCAGTGAAAA 
                     CTTTAAAATGTTCTCGTGTTCACATGTTTCTGCTACCAAAGTTAACAAGAAAGAAAAT 
                     GTTCCTGCTTCTTCACTTTGTGAGAAGCAAGATTATGAAGCCCATCCAAAAATTAAAG 
                     AAATATCTTCAGTAGATTGTATAGCTTTAGTAGATACTATAGATAACTCATCTAAAGC 
                     AGAAAGCATAGATGCTTTAAGTAATAAGCATAGCAAGGAAGAATGTTCTAGTCTCCCA 
                     AGCAAGTCTTTTAATATTGAACACTGTCATCAGAATTCTTCTTCTACTGTTTCATTGG 
                     AAAACGAAGATGTTGGATCATTTAGACAAGAATACCGCCAGCCTTACTTATGTGAAGT 
                     GAAAACAGGCCAAGCTCTAGTTTGTCCTGTTTGTAACGTAGAACAAAAGACTTCAGAT 
                     CTAACCCTGTTCAATGTGCATGTGGATGTTTGCTTAAATAAAAGTTTTATCCAAGAAT 
                     TAAGAAAGGATAAATTTAACCCAGTTAATCAACCCAAAGAAAGCTCCAGAAGTACTGG 
                     TAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGAACAAAAAGGCCAGGATTGATGACA 
                     AAGTACTCAACATCAAAGAAAATAAAACCAAACAATCCCAAACATACCCTTGATATAT 
                     TTTTTAAGTCTGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACCTGGCACAAG 
                     CGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCATCCTCAGAGACT 
                     GGGCCTGTCGCCGTCGATCCAACCCTGCGCCGCCGGATTGAACCTCACGAGTTTGAAG 
                     TGTTCTTTGACCCCCGGGAGCTGAGAAAGGAGACATGCCTGCTGTACGAGATCAACTG 
                     GGGAGGCAGGCACTCCATCTGGAGGCACACCTCTCAGAACACAAATAAGCACGTGGAG 
                     GTGAACTTCATCGAGAAGTTTACCACAGAGCGGTACTTCTGCCCCAATACCAGATGTA 
                     GCATCACATGGTTTCTGAGCTGGTCCCCTTGCGGAGAGTGTAGCAGGGCCATCACCGA 
                     GTTCCTGTCCAGATATCCACACGTGACACTGTTTATCTACATCGCCAGGCTGTATCAC 
                     CACGCAGACCCAAGGAATAGGCAGGGCCTGCGCGATCTGATCAGCTCCGGCGTGACCA 
                     TCCAGATCATGACAGAGCAGGAGTCCGGCTACTGCTGGCGGAACTTCGTGAATTATTC 
                     TCCTAGCAACGAGGCCCACTGGCCTAGGTACCCACACCTGTGGGTGCGCCTGTACGTG 
                     CTGGAGCTGTATTGCATCATCCTGGGCCTGCCCCCTTGTCTGAATATCCTGCGGAGAA 
                     AGCAGCCCCAGCTGACCTTCTTTACAATCGCCCTGCAGTCTTGTCACTATCAGAGGCT 
                     GCCACCCCACATC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAA
                     GATGA 
                     TCTTCTGCTTAGGATGGGACTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAA 
                     GAGAAAATTAACAAAATTATAATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATG 
                     AGCTCAAGAAAGAAAAGCAAGTCAACCAACGAATTGAAAATATGATGCAACAAAAAGC 
                     TCAAATCACCAGCCAACAGCTAAGAAAAGCACAATTACAGGTTGACAGATTTGCAATG 
                     GAATTAGAACAAAGCCGAAATTTGAGCAATACCATAGTGCACATTGACATGGATGCTT 
                     TCTATGCAGCTGTAGAAATGAGGGACAATCCAGAATTGAAGGATAAACCCATTGCTGT 
                     AGGATCAATGAGTATGCTGTCTACTTCAAATTACCATGCAAGGAGATTTGGTGTTCGT 
                     GCAGCCATGCCAGGATTTATTGCTAAGAGGCTGTGCCCACAACTTATAATAGTGCCCC 
                     CCAACTTTGACAAATACCGAGCTGTGAGTAAAGAGGTTAAGGAAATACTTGCTGATTA 
                     TGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCCTACTTGAATATAACAAAGCAC 
                     TTAGAAGAAAGACAAAATTGGCCTGAGGATAAAAGAAGGTATTTCATCAAAATGGGAA 
                     GCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTTAATAAACTGAGTGAGCATGAACG 
                     ATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGCAGCCCCCAGGAGAT 
                     CCTTTCCAAGTGAACTTTGAAGAACAAAACAATCCTCAAATACTCCAAAACTCAGTTG 
                     TTTTTGGAACATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGAATTGAGCAGAA 
                     AACAACACTGACAGCCAGTGCAGGCATTGCCCCAAATACAATGTTAGCAAAAGTATGC 
                     AGTGATAAGAATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAAGCTGTGA 
                     TGGACTTCATCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTTACAGA 
                     GAAAATGTTAAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGGGCA 
                     TTGCTTTCTCTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTGG 
                     GTCTAGGTTCAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAG 
                     GACATTCAGTGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGC 
                     AGTGAGCTTGCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTA 
                     AGTTGAAGAATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGT 
                     TTCTACTGCAGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGAT 
                     GCTGATTTTCCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTC 
                     CCAATGAAGAGGACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGG 
                     AAACCAAGCCCTGTCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTT 
                     GTAAAACCTCTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAA 
                     GGAAATGGAGTCACCAAGATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCA 
                     GACATCACAACCATTCCAAGTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCA 
                     GAGAATTCAGATGACTGTCAGATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGT 
                     GCATCAGTCTGGAAGCCTTGAATAAACATGTAGATGAATGTCTTGATGGACCTTCAAT 
                     CAGTGAAAACTTTAAAATGTTCTCGTGTTCACATGTTTCTGCTACCAAAGTTAACAAG 
                     AAAGAAAATGTTCCTGCTTCTTCACTTTGTGAGAAGCAAGATTATGAAGCCCATCCAA 
                     AAATTAAAGAAATATCTTCAGTAGATTGTATAGCTTTAGTAGATACTATAGATAACTC 
                     ATCTAAAGCAGAAAGCATAGATGCTTTAAGTAATAAGCATAGCAAGGAAGAATGTTCT 
                     AGTCTCCCAAGCAAGTCTTTTAATATTGAACACTGTCATCAGAATTCTTCTTCTACTG 
                     TTTCATTGGAAAACGAAGATGTTGGATCATTTAGACAAGAATACCGCCAGCCTTACTT 
                     ATGTGAAGTGAAAACAGGCCAAGCTCTAGTTTGTCCTGTTTGTAACGTAGAACAAAAG 
                     ACTTCAGATCTAACCCTGTTCAATGTGCATGTGGATGTTTGCTTAAATAAAAGTTTTA 
                     TCCAAGAATTAAGAAAGGATAAATTTAACCCAGTTAATCAACCCAAAGAAAGCTCCAG 
                     AAGTACTGGTAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGAACAAAAAGGCCAGGA 
                     TTGATGACAAAGTACTCAACATCAAAGAAAATAAAACCAAACAATCCCAAACATACCC 
                     TTGATATATTTTTTAAGTCTGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACC 
                     TGGCACAAGCGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCATCC 
                     TCAGAGACTGGGCCTGTCGCCGTCGATCCAACCCTGCGCCGCCGGATTGAACCTCACG 
                     AGTTTGAAGTGTTCTTTGACCCCCGGGAGCTGAGAAAGGAGACATGCCTGCTGTACGA 
                     GATCAACTGGGGAGGCAGGCACTCCATCTGGAGGCACACCTCTCAGAACACAAATAAG 
                     CACGTGGAGGTGAACTTCATCGAGAAGTTTACCACAGAGCGGTACTTCTGCCCCAATA 
                     CCAGATGTAGCATCACATGGTTTCTGAGCTGGTCCCCTTGCGGAGAGTGTAGCAGGGC 
                     CATCACCGAGTTCCTGTCCAGATATCCACACGTGACACTGTTTATCTACATCGCCAGG 
                     CTGTATCACCACGCAGACCCAAGGAATAGGCAGGGCCTGCGCGATCTGATCAGCTCCG 
                     GCGTGACCATCCAGATCATGACAGAGCAGGAGTCCGGCTACTGCTGGCGGAACTTCGT 
                     GAATTATTCTCCTAGCAACGAGGCCCACTGGCCTAGGTACCCACACCTGTGGGTGCGC 
                     CTGTACGTGCTGGAGCTGTATTGCATCATCCTGGGCCTGCCCCCTTGTCTGAATATCC 
                     TGCGGAGAAAGCAGCCCCAGCTGACCTTCTTTACAATCGCCCTGCAGTCTTGTCACTA 
                     TCAGAGGCTGCCACCCCACATC"
     primer_bind     complement(466..486)
                     /label=Primer
     primer_bind     467..504
                     /label=Primer (1)
     primer_bind     487..504
                     /label=Primer (2)
     primer_bind     complement(1102..1138)
                     /label=Primer (3)
     primer_bind     complement(1102..1128)
                     /label=Primer (4)
     CDS             1129..1149
                     /codon_start=1
                     /label=Genscript rAPOBEC1
                     /note="/created_by=jonlevy"
                     /translation="LWATGLK"
     primer_bind     complement(1129..1149)
                     /label=855_genscript_rAPOBEC1-BE3rev
     primer_bind     1129..1149
                     /label=Primer (5)
     CDS             1150..1245
                     /label=BE4max
                     /note="/created_by=User"
     CDS             1246..5346
                     /label=fz hSpCas9
                     /note="hSpCas9"
                     /note="/modified_by=jonlevy"
     primer_bind     complement(1246..1270)
                     /label=905_tadA_rev
     primer_bind     complement(1246..1269)
                     /label=898_fzCas9_D10A-rev-blunt
     primer_bind     complement(1251..1277)
                     /label=896_fzCas9_D10A-rev
     primer_bind     1251..1270
                     /label=908_fzCas9-fwd
     primer_bind     1258..1284
                     /label=895_fzCas9_D10A-fwd
     primer_bind     1270..1290
                     /label=897_fzCas9_D10A-fwd-blunt
     primer_bind     1367..1387
                     /label=LCv2_seq10
     misc_feature    1536..1546
                     /label=PflMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     primer_bind     1819..1838
                     /label=LCv2_seq11
     primer_bind     2371..2390
                     /label=LCv2_seq12
     primer_bind     2870..2888
                     /label=LCv2_seq13
     primer_bind     complement(2931..2961)
                     /label=901_fzCas9_NpuC-rev
     primer_bind     complement(2935..2971)
                     /label=876_fzCas9-resplice
     primer_bind     2962..2984
                     /label=841_FZ_Cas9-C11-fwd
     primer_bind     3364..3383
                     /label=LCv2_seq14
     misc_feature    3717..3727
                     /label=PflMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     primer_bind     3857..3876
                     /label=LCv2_seq15
     primer_bind     4322..4340
                     /label=LCv2_seq16
     misc_feature    4573..5346
                     /label=CTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTG
                     /label=CTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGA 
                     CCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTG 
                     GCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGA 
                     TCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAA 
                     GGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTC 
                     GAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAA 
                     ACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGA 
                     GAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCAC 
                     AAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCC 
                     TGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCC 
                     CATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCC 
                     CCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCA 
                     AAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACG 
                     GATCGACCTGTCTCAGCTGGGAGGTGACAGCGGCGGGAGCGGCGGGAGCGGGGGGAGC 
                     ACTAATCTGAGCGACATCATTGAGAAGGAGACTGGGAAACAGCTGGTCATTCAGGAGT 
                     CCATCCTGATGCTGCCTGAGGAGGTGGAGGAAGTGATCGGCAACAAGCCAGAGTCTGA 
                     CATCCTGGTGCACACCGCCTACGACGAGTCCACAGATGAGAATGTGATGCTGCTGACC 
                     TCTGACGCCCCCGAGTATAAGCCTTGGGCCCTGGTCATCCAGGATTCTAACGGCGAGA 
                     ATAAGATCAAGATGCTGAGCGGAGGATCCGGAGGATCTGGAGGCAGCACCAACCTGTC 
                     TGACATCATCGAGAAGGAGACAGGCAAGCAGCTGGTCATCCAGGAGAGCATCCTGATG 
                     CTGCCCGAAGAAGTCGAAGAAGTGATCGGAAACAAGCCTGAGAGCGATATCCTGGTCC 
                     ATACCGCCTACGACGAGAGTACCGACGAAAATGTGATGCTGCTGACATCCGACGCCCC 
                     AGAGTATAAGCCCTGGGCTCTGGTCATCCAGGATTCCAACGGAGAGAACAAAATCAAA 
                     ATGCTG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGA
                     AAGAA 
                     GGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTG 
                     CTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGC 
                     TGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCT 
                     GGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTAC 
                     TCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGC 
                     AGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAG 
                     CCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTG 
                     GAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGA 
                     GAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCG 
                     GGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAAT 
                     CTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACA 
                     CCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTA 
                     CGAGACACGGATCGACCTGTCTCAGCTGGGAGGTGACAGCGGCGGGAGCGGCGGGAGC 
                     GGGGGGAGCACTAATCTGAGCGACATCATTGAGAAGGAGACTGGGAAACAGCTGGTCA 
                     TTCAGGAGTCCATCCTGATGCTGCCTGAGGAGGTGGAGGAAGTGATCGGCAACAAGCC 
                     AGAGTCTGACATCCTGGTGCACACCGCCTACGACGAGTCCACAGATGAGAATGTGATG 
                     CTGCTGACCTCTGACGCCCCCGAGTATAAGCCTTGGGCCCTGGTCATCCAGGATTCTA 
                     ACGGCGAGAATAAGATCAAGATGCTGAGCGGAGGATCCGGAGGATCTGGAGGCAGCAC 
                     CAACCTGTCTGACATCATCGAGAAGGAGACAGGCAAGCAGCTGGTCATCCAGGAGAGC 
                     ATCCTGATGCTGCCCGAAGAAGTCGAAGAAGTGATCGGAAACAAGCCTGAGAGCGATA 
                     TCCTGGTCCATACCGCCTACGACGAGAGTACCGACGAAAATGTGATGCTGCTGACATC 
                     CGACGCCCCAGAGTATAAGCCCTGGGCTCTGGTCATCCAGGATTCCAACGGAGAGAAC 
                     AAAATCAAAATGCTG"
     misc_feature    4573..4575
                     /label=NG R
                     /note="/created_by=lkoblan"
                     /note="/modified_by=lkoblan"
     misc_feature    4645..4647
                     /label=NG V
                     /note="/created_by=lkoblan"
     primer_bind     4872..4891
                     /label=LCv2_seq17
     misc_feature    4894..4899
                     /label=NG R F
                     /note="/created_by=lkoblan"
     misc_feature    5245..5247
                     /label=NG V
                     /note="/created_by=lkoblan"
                     /note="/modified_by=lkoblan"
     misc_feature    5251..5253
                     /label=NG R
                     /note="/created_by=lkoblan"
     CDS             5359..5409
                     /label=BPNLS
                     /note="/created_by=kzhao"
     misc_feature    5389..5409
                     /label=SV40 NLS
                     /note="/created_by=User"
     primer_bind     5413..5433
                     /label=468_bb-rev-gfpnls
     primer_bind     5454..5476
                     /label=304_FP-JL11_F
     primer_bind     complement(5458..5475)
                     /label=QB0008 BGH Reverse
     primer_bind     complement(5458..5475)
                     /label=Eton BGH Reverse
     primer_bind     complement(5458..5475)
                     /label=Genewiz BGHR
     terminator      5461..5688
                     /label=bGH_PA term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=bGH_PA term"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     5461..5481
                     /label=435_bGH_QPCRF1
     primer_bind     5487..5505
                     /label=436_bGH_QPCRF2
     primer_bind     complement(5610..5634)
                     /label=ACK64
     primer_bind     complement(5610..5634)
                     /label=ACK64 (end)
     primer_bind     complement(5624..5643)
                     /label=438_bGH_QPCRR2
     primer_bind     complement(5629..5647)
                     /label=439_bGH_QPCRR3
     primer_bind     complement(5649..5668)
                     /label=437_bGH_QPCRR1
     primer_bind     5747..5773
                     /label=654_CAG_fwd
     primer_bind     complement(5759..5775)
                     /label=M13-R (-26)
     primer_bind     complement(5759..5775)
                     /label=Eton M13R Reverse
     primer_bind     complement(5759..5775)
                     /label=QB0048 M13 Reverse
     primer_bind     complement(5759..5775)
                     /label=Genewiz M13R
     primer_bind     complement(5772..5795)
                     /label=M13-R (-46)
     primer_bind     complement(5772..5795)
                     /label=QB0108 Tac promoter
     primer_bind     complement(5772..5794)
                     /label=QB0050 M13/pUC Reverse
     promoter        complement(5808..5837)
                     /label=lac prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lac prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(5844..5863)
                     /label=Lac-F (EtonSeq)
     primer_bind     complement(5844..5863)
                     /label=836_Lac-F
     primer_bind     complement(5852..5876)
                     /label=ACK32
     primer_bind     5852..5876
                     /label=ACK21
     misc_difference 5980
                     /label=T>A to remove SapI
                     /note="/created_by=User"
     primer_bind     complement(5990..6007)
                     /label=QB0039 L4440
     primer_bind     complement(6042..6061)
                     /label=VR
     primer_bind     complement(6131..6152)
                     /label=11_pFYF_Seq_Rev
     misc_feature    complement(6146..6765)
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.52%"
     primer_bind     6161..6185
                     /label=ACK10
     primer_bind     complement(6222..6241)
                     /label=BR322-F
     misc_feature    6355^6356
                     /label=A
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=A"
     misc_feature    6357
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     primer_bind     6384..6402
                     /label=QB0766 RepO-F
     primer_bind     6481..6500
                     /label=833_BR322-rev1
     primer_bind     complement(6509..6528)
                     /label=QB0767 RepO-R
     misc_feature    6602
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     primer_bind     6662..6686
                     /label=ACK11
     primer_bind     complement(6662..6681)
                     /label=832_BR322-fwd1
     misc_feature    complement(6920..7780)
                     /label=amp marker
                     /label=nonstandard type:  Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     primer_bind     7161..7185
                     /label=ACK12
     primer_bind     7326..7345
                     /label=ACK563
     primer_bind     7543..7562
                     /label=QB0005 Amp-R
     primer_bind     7628..7655
                     /label=563_Amp_Rev
     primer_bind     complement(7628..7655)
                     /label=562_Amp_Fwd
     primer_bind     7661..7685
                     /label=ACK13
     promoter        complement(7822..7850)
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     7894..7911
                     /label=QB0090 pQE promoter
     primer_bind     complement(7964..7983)
                     /label=QB0092 pRS-marker
     primer_bind     8164..8188
                     /label=ACK14
     primer_bind     8164..8188
                     /label=JLseq006_ACK14
     promoter        join(8171..8403,1..356)
                     /label=CMV_Full
                     /note="/Transferred_From=CMV_Full"
                     /note="/Transferred_Similarity=99.83%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     8171..8214
                     
     primer_bind     complement(8221..8240)
                     /label=QB1110 pSicoR
     misc_feature    8360
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
      481 cctgtcgcag tggacccaac cctgaggaga cggattgagc cccatgaatt tgaagtgttc
      541 tttgacccaa gggagctgag gaaggagaca tgcctgctgt acgagatcaa gtggggcaca
      601 agccacaaga tctggcgcca cagctccaag aacaccacaa agcacgtgga agtgaatttc
      661 atcgagaagt ttacctccga gcggcacttc tgcccctcta ccagctgttc catcacatgg
      721 tttctgtctt ggagcccttg cggcgagtgt tccaaggcca tcaccgagtt cctgtctcag
      781 caccctaacg tgaccctggt catctacgtg gcccggctgt atcaccacat ggaccagcag
      841 aacaggcagg gcctgcgcga tctggtgaat tctggcgtga ccatccagat catgacagcc
      901 ccagagtacg actattgctg gcggaacttc gtgaattatc cacctggcaa ggaggcacac
      961 tggccaagat acccacccct gtggatgaag ctgtatgcac tggagctgca cgcaggaatc
     1021 ctgggcctgc ctccatgtct gaatatcctg cggagaaagc agccccagct gacatttttc
     1081 accattgctc tgcagtcttg tcactatcag cggctgcctc ctcatattct gtgggccaca
     1141 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     1201 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc
     1261 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1321 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1381 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1441 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1501 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1561 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1621 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1681 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1741 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1801 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1861 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1921 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1981 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2041 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2101 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2161 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2221 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2281 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2341 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2401 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2461 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2521 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2581 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2641 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2701 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2761 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2821 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2881 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2941 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3001 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3061 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3121 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3181 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3241 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3301 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3361 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3421 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3481 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3541 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3601 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3661 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3721 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3781 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3841 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3901 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3961 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4021 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4081 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4141 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4201 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4261 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4321 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4381 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4441 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4501 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4561 aaagagtcta tcaggcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4621 gaccctaaga agtacggcgg cttcgtcagc cccaccgtgg cctattctgt gctggtggtg
     4681 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4741 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4801 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4861 gaaaacggcc ggaagagaat gctggcctct gccagattcc tgcagaaggg aaacgaactg
     4921 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4981 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5041 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5101 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5161 gagaatatca tccacctgtt taccctgacc aatctgggag cccctagggc cttcaagtac
     5221 tttgacacca ccatcgaccg gaaggtgtac aggagcacca aagaggtgct ggacgccacc
     5281 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5341 ggtgactctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc caagaagaag
     5401 aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct
     5461 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5521 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     5581 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     5641 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     5701 aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg taatcatggt
     5761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5821 gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcta ttccgcttcc tcgctcactg
     6001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     7921 gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc tgctctgatg
     7981 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     8041 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
     8101 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
     8161 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     8221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     8281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     8341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     8401 atc
//
