LOCUS       Exported                8313 bp ds-DNA     circular UNA 30-OCT-2020
DEFINITION  bpNLS - SapI x2 - BE4max.
ACCESSION   urn.local...383-cl9ur8m
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 11, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     198..211
                     /label=ACK15
     primer_bind     198..211
                     /label=JLseq007_ACK15
     primer_bind     234..253
                     /label=LCv2_seq1
     primer_bind     261..285
                     /label=ACK15
     primer_bind     261..285
                     /label=JLseq007_ACK15
     promoter        275..356
                     /label=CMV prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=CMV prom"
                     /note="/Transferred_Similarity=97.56%"
     primer_bind     286..306
                     /label=Genewiz CMV-Forward
     primer_bind     286..306
                     /label=Eton CMV-Forward
     primer_bind     286..306
                     /label=QB0012 CMV Forward
     primer_bind     293..314
                     /label=QB0976 pRLuc-MCS-F
     primer_bind     complement(331..360)
                     /label=352_CMV_Rev2
     primer_bind     377..396
                     /label=T7
     primer_bind     377..396
                     /label=Genewiz T7
     primer_bind     377..396
                     /label=Eton T7 Promoter
     primer_bind     377..396
                     /label=QB0106 T7
     primer_bind     377..396
                     /label=T7 promoter
     promoter        377..395
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(382..408)
                     /label=197_pCMV_bb_F
     primer_bind     complement(385..408)
                     /label=203_bb_NpuC_R
     primer_bind     complement(389..447)
                     /label=875_Genscript_APOBEC-bpnls-rev
     primer_bind     389..428
                     /label=903_GS_NpuN-fwd
     primer_bind     complement(389..408)
                     
     primer_bind     complement(389..408)
                     
     misc_feature    406..408
                     /label=USER1
                     /note="/created_by=User"
     CDS             409..465
                     /label=NLS
                     /note="/created_by=User"
     misc_feature    466..1059
                     /label=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCT
                     /label=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCT 
                     TAGGATGGGACTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATT 
                     AACAAAATTATAATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGA 
                     AAGAAAAGCAAGTCAACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCAC 
                     CAGCCAACAGCTAAGAAAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAA 
                     CAAAGCCGAAATTTGAGCAATACCATAGTGCACATTGACATGGATGCTTTCTATGCAG 
                     CTGTAGAAATGAGGGACAATCCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAAT 
                     GAGTATGCTGTCTACTTCAAATTACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATG 
                     CCAGGATTTATTGCTAAGAGGCTGTGCCCACAACTTATAATAGTGCCCCCCAACTTTG 
                     ACAAATACCGAGCTGTGAGTAAAGAGGTTAAGGAAATACTTGCTGATTATGATCCCAA 
                     TTTTATGGCCATGAGTCTTGATGAAGCCTACTTGAATATAACAAAGCACTTAGAAGAA 
                     AGACAAAATTGGCCTGAGGATAAAAGAAGGTATTTCATCAAAATGGGAAGCTCTGTAG 
                     AAAATGATAATCCAGGAAAGGAAGTTAATAAACTGAGTGAGCATGAACGATCCATCTC 
                     TCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGCAGCCCCCAGGAGATCCTTTCCAA 
                     GTGAACTTTGAAGAACAAAACAATCCTCAAATACTCCAAAACTCAGTTGTTTTTGGAA 
                     CATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGAATTGAGCAGAAAACAACACT 
                     GACAGCCAGTGCAGGCATTGCCCCAAATACAATGTTAGCAAAAGTATGCAGTGATAAG 
                     AATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAAGCTGTGATGGACTTCA 
                     TCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTTACAGAGAAAATGTT 
                     AAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGGGCATTGCTTTCT 
                     CTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTGGGTCTAGGTT 
                     CAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAGGACATTCAG 
                     TGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGCAGTGAGCTT 
                     GCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTAAGTTGAAGA 
                     ATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGTTTCTACTGC 
                     AGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGATGCTGATTTT 
                     CCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTCCCAATGAAG 
                     AGGACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGGAAACCAAGC 
                     CCTGTCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTTGTAAAACCT 
                     CTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAAGGAAATGGA 
                     GTCACCAAGATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCAGACATCACA 
                     ACCATTCCAAGTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCAGAGAATTCA 
                     GATGACTGTCAGATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGTGCATCAGTC 
                     TGGAAGCCTTGAATAAACATGTAGATGAATGTCTTGATGGACCTTCAATCAGTGAAAA 
                     CTTTAAAATGTTCTCGTGTTCACATGTTTCTGCTACCAAAGTTAACAAGAAAGAAAAT 
                     GTTCCTGCTTCTTCACTTTGTGAGAAGCAAGATTATGAAGCCCATCCAAAAATTAAAG 
                     AAATATCTTCAGTAGATTGTATAGCTTTAGTAGATACTATAGATAACTCATCTAAAGC 
                     AGAAAGCATAGATGCTTTAAGTAATAAGCATAGCAAGGAAGAATGTTCTAGTCTCCCA 
                     AGCAAGTCTTTTAATATTGAACACTGTCATCAGAATTCTTCTTCTACTGTTTCATTGG 
                     AAAACGAAGATGTTGGATCATTTAGACAAGAATACCGCCAGCCTTACTTATGTGAAGT 
                     GAAAACAGGCCAAGCTCTAGTTTGTCCTGTTTGTAACGTAGAACAAAAGACTTCAGAT 
                     CTAACCCTGTTCAATGTGCATGTGGATGTTTGCTTAAATAAAAGTTTTATCCAAGAAT 
                     TAAGAAAGGATAAATTTAACCCAGTTAATCAACCCAAAGAAAGCTCCAGAAGTACTGG 
                     TAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGAACAAAAAGGCCAGGATTGATGACA 
                     AAGTACTCAACATCAAAGAAAATAAAACCAAACAATCCCAAACATACCCTTGATATAT 
                     TTTTTAAGTCTGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACCTGGCACAAG 
                     CGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCATCCTCAGAGACT 
                     GGGCCTGTCGCCGTCGATCCAACCCTGCGCCGCCGGATTGAACCTCACGAGTTTGAAG 
                     TGTTCTTTGACCCCCGGGAGCTGAGAAAGGAGACATGCCTGCTGTACGAGATCAACTG 
                     GGGAGGCAGGCACTCCATCTGGAGGCACACCTCTCAGAACACAAATAAGCACGTGGAG 
                     GTGAACTTCATCGAGAAGTTTACCACAGAGCGGTACTTCTGCCCCAATACCAGATGTA 
                     GCATCACATGGTTTCTGAGCTGGTCCCCTTGCGGAGAGTGTAGCAGGGCCATCACCGA 
                     GTTCCTGTCCAGATATCCACACGTGACACTGTTTATCTACATCGCCAGGCTGTATCAC 
                     CACGCAGACCCAAGGAATAGGCAGGGCCTGCGCGATCTGATCAGCTCCGGCGTGACCA 
                     TCCAGATCATGACAGAGCAGGAGTCCGGCTACTGCTGGCGGAACTTCGTGAATTATTC 
                     TCCTAGCAACGAGGCCCACTGGCCTAGGTACCCACACCTGTGGGTGCGCCTGTACGTG 
                     CTGGAGCTGTATTGCATCATCCTGGGCCTGCCCCCTTGTCTGAATATCCTGCGGAGAA 
                     AGCAGCCCCAGCTGACCTTCTTTACAATCGCCCTGCAGTCTTGTCACTATCAGAGGCT 
                     GCCACCCCACATCCTGTGGGCCACAGGCCTGAAG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAA
                     GATGA 
                     TCTTCTGCTTAGGATGGGACTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAA 
                     GAGAAAATTAACAAAATTATAATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATG 
                     AGCTCAAGAAAGAAAAGCAAGTCAACCAACGAATTGAAAATATGATGCAACAAAAAGC 
                     TCAAATCACCAGCCAACAGCTAAGAAAAGCACAATTACAGGTTGACAGATTTGCAATG 
                     GAATTAGAACAAAGCCGAAATTTGAGCAATACCATAGTGCACATTGACATGGATGCTT 
                     TCTATGCAGCTGTAGAAATGAGGGACAATCCAGAATTGAAGGATAAACCCATTGCTGT 
                     AGGATCAATGAGTATGCTGTCTACTTCAAATTACCATGCAAGGAGATTTGGTGTTCGT 
                     GCAGCCATGCCAGGATTTATTGCTAAGAGGCTGTGCCCACAACTTATAATAGTGCCCC 
                     CCAACTTTGACAAATACCGAGCTGTGAGTAAAGAGGTTAAGGAAATACTTGCTGATTA 
                     TGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCCTACTTGAATATAACAAAGCAC 
                     TTAGAAGAAAGACAAAATTGGCCTGAGGATAAAAGAAGGTATTTCATCAAAATGGGAA 
                     GCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTTAATAAACTGAGTGAGCATGAACG 
                     ATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGCAGCCCCCAGGAGAT 
                     CCTTTCCAAGTGAACTTTGAAGAACAAAACAATCCTCAAATACTCCAAAACTCAGTTG 
                     TTTTTGGAACATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGAATTGAGCAGAA 
                     AACAACACTGACAGCCAGTGCAGGCATTGCCCCAAATACAATGTTAGCAAAAGTATGC 
                     AGTGATAAGAATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAAGCTGTGA 
                     TGGACTTCATCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTTACAGA 
                     GAAAATGTTAAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGGGCA 
                     TTGCTTTCTCTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTGG 
                     GTCTAGGTTCAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAG 
                     GACATTCAGTGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGC 
                     AGTGAGCTTGCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTA 
                     AGTTGAAGAATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGT 
                     TTCTACTGCAGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGAT 
                     GCTGATTTTCCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTC 
                     CCAATGAAGAGGACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGG 
                     AAACCAAGCCCTGTCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTT 
                     GTAAAACCTCTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAA 
                     GGAAATGGAGTCACCAAGATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCA 
                     GACATCACAACCATTCCAAGTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCA 
                     GAGAATTCAGATGACTGTCAGATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGT 
                     GCATCAGTCTGGAAGCCTTGAATAAACATGTAGATGAATGTCTTGATGGACCTTCAAT 
                     CAGTGAAAACTTTAAAATGTTCTCGTGTTCACATGTTTCTGCTACCAAAGTTAACAAG 
                     AAAGAAAATGTTCCTGCTTCTTCACTTTGTGAGAAGCAAGATTATGAAGCCCATCCAA 
                     AAATTAAAGAAATATCTTCAGTAGATTGTATAGCTTTAGTAGATACTATAGATAACTC 
                     ATCTAAAGCAGAAAGCATAGATGCTTTAAGTAATAAGCATAGCAAGGAAGAATGTTCT 
                     AGTCTCCCAAGCAAGTCTTTTAATATTGAACACTGTCATCAGAATTCTTCTTCTACTG 
                     TTTCATTGGAAAACGAAGATGTTGGATCATTTAGACAAGAATACCGCCAGCCTTACTT 
                     ATGTGAAGTGAAAACAGGCCAAGCTCTAGTTTGTCCTGTTTGTAACGTAGAACAAAAG 
                     ACTTCAGATCTAACCCTGTTCAATGTGCATGTGGATGTTTGCTTAAATAAAAGTTTTA 
                     TCCAAGAATTAAGAAAGGATAAATTTAACCCAGTTAATCAACCCAAAGAAAGCTCCAG 
                     AAGTACTGGTAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGAACAAAAAGGCCAGGA 
                     TTGATGACAAAGTACTCAACATCAAAGAAAATAAAACCAAACAATCCCAAACATACCC 
                     TTGATATATTTTTTAAGTCTGGAGGATCTAGCGGAGGCTCCTCTGGCTCTGAGACACC 
                     TGGCACAAGCGAGAGCGCAACACCTGAAAGCAGCGGGGGCAGCAGCGGGGGGTCATCC 
                     TCAGAGACTGGGCCTGTCGCCGTCGATCCAACCCTGCGCCGCCGGATTGAACCTCACG 
                     AGTTTGAAGTGTTCTTTGACCCCCGGGAGCTGAGAAAGGAGACATGCCTGCTGTACGA 
                     GATCAACTGGGGAGGCAGGCACTCCATCTGGAGGCACACCTCTCAGAACACAAATAAG 
                     CACGTGGAGGTGAACTTCATCGAGAAGTTTACCACAGAGCGGTACTTCTGCCCCAATA 
                     CCAGATGTAGCATCACATGGTTTCTGAGCTGGTCCCCTTGCGGAGAGTGTAGCAGGGC 
                     CATCACCGAGTTCCTGTCCAGATATCCACACGTGACACTGTTTATCTACATCGCCAGG 
                     CTGTATCACCACGCAGACCCAAGGAATAGGCAGGGCCTGCGCGATCTGATCAGCTCCG 
                     GCGTGACCATCCAGATCATGACAGAGCAGGAGTCCGGCTACTGCTGGCGGAACTTCGT 
                     GAATTATTCTCCTAGCAACGAGGCCCACTGGCCTAGGTACCCACACCTGTGGGTGCGC 
                     CTGTACGTGCTGGAGCTGTATTGCATCATCCTGGGCCTGCCCCCTTGTCTGAATATCC 
                     TGCGGAGAAAGCAGCCCCAGCTGACCTTCTTTACAATCGCCCTGCAGTCTTGTCACTA 
                     TCAGAGGCTGCCACCCCACATCCTGTGGGCCACAGGCCTGAAG"
     misc_feature    466..1059
                     /label=ORF 1 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/codon_start=1"
                     /note="/created_by=ORF Finder"
     gene            466..1059
                     /label=Ordered from IDT as Gblock
                     /note="/created_by=marbab"
                     /note="/modified_by=marbab"
     CDS             466..1059
                     /label=eA3A N57G - GenScript codon optimization
                     /note="/created_by=marbab"
                     /note="/modified_by=marbab"
     misc_feature    466..471
                     /label=092518_eA3A_FwU-GS
                     /label=nonstandard type:  user
                     /note="/created_by=marbab"
     misc_feature    631..633
                     /label=N57G
                     /label=nonstandard type:  user
                     /note="/created_by=marbab"
                     /note="/modified_by=marbab"
     primer_bind     903..914
                     /label=LCv2_seq14
     CDS             1060..5256
                     /label=BE4max
                     /note="/created_by=User"
     CDS             1156..5256
                     /label=fz hSpCas9
                     /note="hSpCas9"
                     /note="/modified_by=jonlevy"
     primer_bind     complement(1156..1180)
                     /label=905_tadA_rev
     primer_bind     complement(1156..1179)
                     /label=898_fzCas9_D10A-rev-blunt
     primer_bind     1161..1180
                     /label=908_fzCas9-fwd
     primer_bind     1277..1297
                     /label=LCv2_seq10
     primer_bind     1729..1748
                     /label=LCv2_seq11
     primer_bind     2281..2300
                     /label=LCv2_seq12
     primer_bind     2780..2798
                     /label=LCv2_seq13
     primer_bind     complement(2841..2871)
                     /label=901_fzCas9_NpuC-rev
     primer_bind     complement(2845..2881)
                     /label=876_fzCas9-resplice
     primer_bind     2872..2894
                     /label=841_FZ_Cas9-C11-fwd
     primer_bind     3274..3293
                     /label=LCv2_seq14
     primer_bind     3767..3786
                     /label=LCv2_seq15
     primer_bind     4232..4250
                     /label=LCv2_seq16
     primer_bind     4782..4801
                     /label=LCv2_seq17
     misc_feature    5256^5257
                     /label=AGCGGCGGGAGCGGCGGGAGCGGGGGGAGCACTAATCTGAGCGACAT
                     /label=AGCGGCGGGAGCGGCGGGAGCGGGGGGAGCACTAATCTGAGCGACATCAT 
                     TGAGAAGGAGACTGGGAAACAGCTGGTCATTCAGGAGTCCATCCTGATGCTGCCTGAG 
                     GAGGTGGAGGAAGTGATCGGCAACAAGCCAGAGTCTGACATCCTGGTGCACACCGCCT 
                     ACGACGAGTCCACAGATGAGAATGTGATGCTGCTGACCTCTGACGCCCCCGAGTATAA 
                     GCCTTGGGCCCTGGTCATCCAGGATTCTAACGGCGAGAATAAGATCAAGATGCTGAGC 
                     GGAGGATCCGGAGGATCTGGAGGCAGCACCAACCTGTCTGACATCATCGAGAAGGAGA 
                     CAGGCAAGCAGCTGGTCATCCAGGAGAGCATCCTGATGCTGCCCGAAGAAGTCGAAGA 
                     AGTGATCGGAAACAAGCCTGAGAGCGATATCCTGGTCCATACCGCCTACGACGAGAGT 
                     ACCGACGAAAATGTGATGCTGCTGACATCCGACGCCCCAGAGTATAAGCCCTGGGCTC 
                     TGGTCATCCAGGATTCCAACGGAGAGAACAAAATCAAAATGCTG
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=AGCGGCGGGAGCGGCGGGAGCGGGGGGAGCACTAAT
                     CTGAG 
                     CGACATCATTGAGAAGGAGACTGGGAAACAGCTGGTCATTCAGGAGTCCATCCTGATG 
                     CTGCCTGAGGAGGTGGAGGAAGTGATCGGCAACAAGCCAGAGTCTGACATCCTGGTGC 
                     ACACCGCCTACGACGAGTCCACAGATGAGAATGTGATGCTGCTGACCTCTGACGCCCC 
                     CGAGTATAAGCCTTGGGCCCTGGTCATCCAGGATTCTAACGGCGAGAATAAGATCAAG 
                     ATGCTGAGCGGAGGATCCGGAGGATCTGGAGGCAGCACCAACCTGTCTGACATCATCG 
                     AGAAGGAGACAGGCAAGCAGCTGGTCATCCAGGAGAGCATCCTGATGCTGCCCGAAGA 
                     AGTCGAAGAAGTGATCGGAAACAAGCCTGAGAGCGATATCCTGGTCCATACCGCCTAC 
                     GACGAGAGTACCGACGAAAATGTGATGCTGCTGACATCCGACGCCCCAGAGTATAAGC 
                     CCTGGGCTCTGGTCATCCAGGATTCCAACGGAGAGAACAAAATCAAAATGCTG"
     CDS             5269..5319
                     /label=BPNLS
                     /note="/created_by=kzhao"
     misc_feature    5299..5319
                     /label=SV40 NLS
                     /note="/created_by=User"
     primer_bind     5323..5343
                     /label=468_bb-rev-gfpnls
     primer_bind     5364..5386
                     /label=304_FP-JL11_F
     primer_bind     complement(5368..5385)
                     /label=QB0008 BGH Reverse
     primer_bind     complement(5368..5385)
                     /label=Eton BGH Reverse
     primer_bind     complement(5368..5385)
                     /label=Genewiz BGHR
     terminator      5371..5598
                     /label=bGH_PA term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=bGH_PA term"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     5371..5391
                     /label=435_bGH_QPCRF1
     primer_bind     5397..5415
                     /label=436_bGH_QPCRF2
     primer_bind     complement(5520..5544)
                     /label=ACK64
     primer_bind     complement(5520..5544)
                     /label=ACK64 (end)
     primer_bind     complement(5534..5553)
                     /label=438_bGH_QPCRR2
     primer_bind     complement(5539..5557)
                     /label=439_bGH_QPCRR3
     primer_bind     complement(5559..5578)
                     /label=437_bGH_QPCRR1
     primer_bind     5657..5683
                     /label=654_CAG_fwd
     primer_bind     complement(5669..5685)
                     /label=M13-R (-26)
     primer_bind     complement(5669..5685)
                     /label=Eton M13R Reverse
     primer_bind     complement(5669..5685)
                     /label=QB0048 M13 Reverse
     primer_bind     complement(5669..5685)
                     /label=Genewiz M13R
     primer_bind     complement(5682..5705)
                     /label=M13-R (-46)
     primer_bind     complement(5682..5705)
                     /label=QB0108 Tac promoter
     primer_bind     complement(5682..5704)
                     /label=QB0050 M13/pUC Reverse
     promoter        complement(5718..5747)
                     /label=lac prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lac prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(5754..5773)
                     /label=Lac-F (EtonSeq)
     primer_bind     complement(5754..5773)
                     /label=836_Lac-F
     primer_bind     complement(5762..5786)
                     /label=ACK32
     primer_bind     5762..5786
                     /label=ACK21
     misc_difference 5890
                     /label=T>A to remove SapI
                     /note="/created_by=User"
     primer_bind     complement(5900..5917)
                     /label=QB0039 L4440
     primer_bind     complement(5952..5971)
                     /label=VR
     primer_bind     complement(6041..6062)
                     /label=11_pFYF_Seq_Rev
     misc_feature    complement(6056..6675)
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.52%"
     primer_bind     6071..6095
                     /label=ACK10
     primer_bind     complement(6132..6151)
                     /label=BR322-F
     misc_feature    6265^6266
                     /label=A
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=A"
     misc_feature    6267
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     primer_bind     6294..6312
                     /label=QB0766 RepO-F
     primer_bind     6391..6410
                     /label=833_BR322-rev1
     primer_bind     complement(6419..6438)
                     /label=QB0767 RepO-R
     misc_feature    6512
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     primer_bind     6572..6596
                     /label=ACK11
     primer_bind     complement(6572..6591)
                     /label=832_BR322-fwd1
     misc_feature    complement(6830..7690)
                     /label=amp marker
                     /label=nonstandard type:  Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     primer_bind     7071..7095
                     /label=ACK12
     primer_bind     7236..7255
                     /label=ACK563
     primer_bind     7453..7472
                     /label=QB0005 Amp-R
     primer_bind     7538..7565
                     /label=563_Amp_Rev
     primer_bind     complement(7538..7565)
                     /label=562_Amp_Fwd
     primer_bind     7571..7595
                     /label=ACK13
     promoter        complement(7732..7760)
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     7804..7821
                     /label=QB0090 pQE promoter
     primer_bind     complement(7874..7893)
                     /label=QB0092 pRS-marker
     primer_bind     8074..8098
                     /label=ACK14
     primer_bind     8074..8098
                     /label=JLseq006_ACK14
     promoter        join(8081..8313,1..356)
                     /label=CMV_Full
                     /note="/Transferred_From=CMV_Full"
                     /note="/Transferred_Similarity=99.83%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     8081..8124
                     
     primer_bind     complement(8131..8150)
                     /label=QB1110 pSicoR
     misc_feature    8270
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcgaggc aagcccagca
      481 agcggcccac ggcacctgat ggacccccac attttcacca gcaacttcaa caacggaatc
      541 ggacgacaca agacctacct gtgctatgag gtggagaggc tggacaacgg cacatccgtg
      601 aagatggatc agcacagagg cttcctgcac ggccaggcca agaatctgct gtgcggcttc
      661 tacggccggc acgcagagct gcgctttctg gacctggtgc catctctgca gctggatccc
      721 gcccagatct atagggtgac ctggttcatc agctggtccc cttgctttag ctggggatgt
      781 gcaggagagg tgagggcctt cctgcaggag aacacacacg tgaggctgag aatctttgcc
      841 gcccggatct acgactatga tcccctgtac aaggaggccc tgcagatgct gagggacgca
      901 ggagcccagg tgtctatcat gacctatgat gagttcaagc actgctggga cacatttgtg
      961 gatcaccagg gctgtccctt tcagccttgg gacggcctgg acgagcattc ccaggcactg
     1021 agcggacggc tgagggcaat tctgcagaac cagggcaatt ctggaggatc tagcggagga
     1081 tcctctggca gcgagacacc aggaacaagc gagtcagcaa caccagagag cagtggcggc
     1141 agcagcggcg gcagcgacaa gaagtacagc atcggcctgg ccatcggcac caactctgtg
     1201 ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa ggtgctgggc
     1261 aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt cgacagcggc
     1321 gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac cagacggaag
     1381 aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt ggacgacagc
     1441 ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca cgagcggcac
     1501 cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc caccatctac
     1561 cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct gatctatctg
     1621 gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga cctgaacccc
     1681 gacaacagcg acgtggacaa gctgttcatc cagctggtgc agacctacaa ccagctgttc
     1741 gaggaaaacc ccatcaacgc cagcggcgtg gacgccaagg ccatcctgtc tgccagactg
     1801 agcaagagca gacggctgga aaatctgatc gcccagctgc ccggcgagaa gaagaatggc
     1861 ctgttcggaa acctgattgc cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc
     1921 gacctggccg aggatgccaa actgcagctg agcaaggaca cctacgacga cgacctggac
     1981 aacctgctgg cccagatcgg cgaccagtac gccgacctgt ttctggccgc caagaacctg
     2041 tccgacgcca tcctgctgag cgacatcctg agagtgaaca ccgagatcac caaggccccc
     2101 ctgagcgcct ctatgatcaa gagatacgac gagcaccacc aggacctgac cctgctgaaa
     2161 gctctcgtgc ggcagcagct gcctgagaag tacaaagaga ttttcttcga ccagagcaag
     2221 aacggctacg ccggctacat tgacggcgga gccagccagg aagagttcta caagttcatc
     2281 aagcccatcc tggaaaagat ggacggcacc gaggaactgc tcgtgaagct gaacagagag
     2341 gacctgctgc ggaagcagcg gaccttcgac aacggcagca tcccccacca gatccacctg
     2401 ggagagctgc acgccattct gcggcggcag gaagattttt acccattcct gaaggacaac
     2461 cgggaaaaga tcgagaagat cctgaccttc cgcatcccct actacgtggg ccctctggcc
     2521 aggggaaaca gcagattcgc ctggatgacc agaaagagcg aggaaaccat caccccctgg
     2581 aacttcgagg aagtggtgga caagggcgct tccgcccaga gcttcatcga gcggatgacc
     2641 aacttcgata agaacctgcc caacgagaag gtgctgccca agcacagcct gctgtacgag
     2701 tacttcaccg tgtataacga gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag
     2761 cccgccttcc tgagcggcga gcagaaaaag gccatcgtgg acctgctgtt caagaccaac
     2821 cggaaagtga ccgtgaagca gctgaaagag gactacttca agaaaatcga gtgcttcgac
     2881 tccgtggaaa tctccggcgt ggaagatcgg ttcaacgcct ccctgggcac ataccacgat
     2941 ctgctgaaaa ttatcaagga caaggacttc ctggacaatg aggaaaacga ggacattctg
     3001 gaagatatcg tgctgaccct gacactgttt gaggacagag agatgatcga ggaacggctg
     3061 aaaacctatg cccacctgtt cgacgacaaa gtgatgaagc agctgaagcg gcggagatac
     3121 accggctggg gcaggctgag ccggaagctg atcaacggca tccgggacaa gcagtccggc
     3181 aagacaatcc tggatttcct gaagtccgac ggcttcgcca acagaaactt catgcagctg
     3241 atccacgacg acagcctgac ctttaaagag gacatccaga aagcccaggt gtccggccag
     3301 ggcgatagcc tgcacgagca cattgccaat ctggccggca gccccgccat taagaagggc
     3361 atcctgcaga cagtgaaggt ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc
     3421 gagaacatcg tgatcgaaat ggccagagag aaccagacca cccagaaggg acagaagaac
     3481 agccgcgaga gaatgaagcg gatcgaagag ggcatcaaag agctgggcag ccagatcctg
     3541 aaagaacacc ccgtggaaaa cacccagctg cagaacgaga agctgtacct gtactacctg
     3601 cagaatgggc gggatatgta cgtggaccag gaactggaca tcaaccggct gtccgactac
     3661 gatgtggacc atatcgtgcc tcagagcttt ctgaaggacg actccatcga caacaaggtg
     3721 ctgaccagaa gcgacaagaa ccggggcaag agcgacaacg tgccctccga agaggtcgtg
     3781 aagaagatga agaactactg gcggcagctg ctgaacgcca agctgattac ccagagaaag
     3841 ttcgacaatc tgaccaaggc cgagagaggc ggcctgagcg aactggataa ggccggcttc
     3901 atcaagagac agctggtgga aacccggcag atcacaaagc acgtggcaca gatcctggac
     3961 tcccggatga acactaagta cgacgagaat gacaagctga tccgggaagt gaaagtgatc
     4021 accctgaagt ccaagctggt gtccgatttc cggaaggatt tccagtttta caaagtgcgc
     4081 gagatcaaca actaccacca cgcccacgac gcctacctga acgccgtcgt gggaaccgcc
     4141 ctgatcaaaa agtaccctaa gctggaaagc gagttcgtgt acggcgacta caaggtgtac
     4201 gacgtgcgga agatgatcgc caagagcgag caggaaatcg gcaaggctac cgccaagtac
     4261 ttcttctaca gcaacatcat gaactttttc aagaccgaga ttaccctggc caacggcgag
     4321 atccggaagc ggcctctgat cgagacaaac ggcgaaaccg gggagatcgt gtgggataag
     4381 ggccgggatt ttgccaccgt gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa
     4441 aagaccgagg tgcagacagg cggcttcagc aaagagtcta tcctgcccaa gaggaacagc
     4501 gataagctga tcgccagaaa gaaggactgg gaccctaaga agtacggcgg cttcgacagc
     4561 cccaccgtgg cctattctgt gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa
     4621 ctgaagagtg tgaaagagct gctggggatc accatcatgg aaagaagcag cttcgagaag
     4681 aatcccatcg actttctgga agccaagggc tacaaagaag tgaaaaagga cctgatcatc
     4741 aagctgccta agtactccct gttcgagctg gaaaacggcc ggaagagaat gctggcctct
     4801 gccggcgaac tgcagaaggg aaacgaactg gccctgccct ccaaatatgt gaacttcctg
     4861 tacctggcca gccactatga gaagctgaag ggctcccccg aggataatga gcagaaacag
     4921 ctgtttgtgg aacagcacaa gcactacctg gacgagatca tcgagcagat cagcgagttc
     4981 tccaagagag tgatcctggc cgacgctaat ctggacaaag tgctgtccgc ctacaacaag
     5041 caccgggata agcccatcag agagcaggcc gagaatatca tccacctgtt taccctgacc
     5101 aatctgggag cccctgccgc cttcaagtac tttgacacca ccatcgaccg gaagaggtac
     5161 accagcacca aagaggtgct ggacgccacc ctgatccacc agagcatcac cggcctgtac
     5221 gagacacgga tcgacctgtc tcagctggga ggtgactctg gcggctcaaa aagaaccgcc
     5281 gacggcagcg aattcgagcc caagaagaag aggaaagtct aaccggtcat catcaccatc
     5341 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5401 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5461 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     5521 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     5581 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
     5641 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5701 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
     5761 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5881 ttgggcgcta ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7681 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7741 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7801 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag
     7861 ggtcgactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7921 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7981 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8041 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     8101 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8161 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8221 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8281 aaactgccca cttggcagta catcaagtgt atc
//
