LOCUS       Exported                7304 bp ds-DNA     circular SYN 17-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 18, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, same as MSCV, forward 
                     primer"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, 5' of MCS in pBABE vectors, 
                     forward primer"
     CDS             1432..2154
                     /codon_start=1
                     /label=BCL2
                     /note="BCL2"
                     /translation="MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPA
                     PGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDF
                     SRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVN
                     REMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLS
                     LALVGACITLGAYLGHK*"
     misc_feature    2205..2757
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2277..2296)
                     /label=IRES-R
                     /note="5' end of IRES, reverse primer"
     primer_bind     2576..2595
                     /label=IRES-F
                     /note="3' end of IRES, forward primer"
     CDS             2769..3599
                     /codon_start=1
                     /label=huCD2
                     /note="huCD2"
                     /translation="MVSFPCKFVASFLLIFNVSSKGAVSKEITNALETWGALGQDINLD
                     IPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQD
                     IYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDG
                     KHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLDIYLIIGICGGGS
                     LLMVFVALLVFYITKRKKQRSRRNDEELETRAHRVATEERGRKPHQIPASTPQN"
     LTR             3666..4180
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(4349..4365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4373..4389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4397..4427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4442..4463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4580..4597)
                     /label=L4440
                     /note="5' of MCS in L4440 vector, forward primer"
     rep_origin      complement(4751..5339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4831..4850)
                     /label=pBR322ori-F
                     /note="pBRS322 origin, forward primer"
     CDS             complement(5510..6370)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6133..6152
                     /label=Amp-R
                     /note="5' end of ampicillin resistance gene, reverse 
                     primer"
     promoter        complement(6371..6475)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6543..6561
                     /label=pBRforEco
                     /note="In pBR322, upsteam of EcoRI site, forward primer"
     primer_bind     complement(6599..6621)
                     /label=pGEX 3'
                     /note="3' of MCS in pGEX vectors, reverse primer"
     primer_bind     6721..6740
                     /label=pRS-marker
                     /note="To sequence yeast selectable marker in pRS vectors"
     primer_bind     6934..6956
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7084..7103
                     /label=pBRrevBam
                     /note="In pBR322 tet region, downstream of BamHI, reverse 
                     primer"
     LTR             join(7303..7304,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaaccac catggcgcac
     1441 gctgggagaa cagggtacga taaccgggag atagtgatga agtacatcca ttataagctg
     1501 tcgcagaggg gctacgagtg ggatgcggga gatgtgggag ccgctcctcc tggtgccgct
     1561 cccgcaccgg gcatcttctc ctcccagcct gggcacacgc ctcatccagc cgcatcccgg
     1621 gatcctgtcg ccaggacctc gcctctgcag acaccggctg ctcctggagc tgctgcagga
     1681 cctgcgctca gcccggtgcc acctgtggtc cacctgaccc tccgccaggc cggcgacgac
     1741 ttctcccgcc gctaccgccg cgacttcgcc gagatgtcca gccagctgca cctgacgccc
     1801 ttcaccgcgc ggggacgctt tgccacggtg gtggaggagc tcttcaggga cggggtgaac
     1861 tgggggagga ttgtggcctt ctttgagttc ggtggggtca tgtgtgtgga gagcgtcaac
     1921 cgggagatgt cgcccctggt ggacaacatc gccctgtgga tgactgagta cctgaaccgg
     1981 cacctgcaca cctggatcca ggataacgga ggctgggatg cctttgtgga actgtacggc
     2041 cccagcatgc ggcctctgtt tgatttctcc tggctgtctc tgaagactct gctcagtttg
     2101 gccctggtgg gagcttgcat caccctgggt gcctatctgg gccacaagtg atagaacgcg
     2161 gccgcaccgg tggatcctac gtagaattcc gccccccccc cctaacgtta ctggccgaag
     2221 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     2281 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     2341 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     2401 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     2461 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     2521 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     2581 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     2641 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     2701 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     2761 ccacaaccat ggtgagcttt ccatgtaaat ttgtagccag cttccttctg attttcaatg
     2821 tttcttccaa aggtgcagtc tccaaagaga ttacgaatgc cttggaaacc tggggtgcct
     2881 tgggtcagga catcaacttg gacattccta gttttcaaat gagtgatgat attgacgata
     2941 taaaatggga aaaaacttca gacaagaaaa agattgcaca attcagaaaa gagaaagaga
     3001 ctttcaagga aaaagataca tataagctat ttaaaaatgg aactctgaaa attaagcatc
     3061 tgaagaccga tgatcaggat atctacaagg tatcaatata tgatacaaaa ggaaaaaatg
     3121 tgttggaaaa aatatttgat ttgaagattc aagagagggt ctcaaaacca aagatctcct
     3181 ggacttgtat caacacaacc ctgacctgtg aggtaatgaa tggaactgac cccgaattaa
     3241 acctgtatca agatgggaaa catctaaaac tttctcagag ggtcatcaca cacaagtgga
     3301 ccaccagcct gagtgcaaaa ttcaagtgca cagcagggaa caaagtcagc aaggaatcca
     3361 gtgtcgagcc tgtcagctgt ccagagaaag gtctggacat ctatctcatc attggcatat
     3421 gtggaggagg cagcctcttg atggtctttg tggcactgct cgttttctat atcaccaaaa
     3481 ggaaaaaaca gaggagtcgg agaaatgatg aggagctgga gacaagagcc cacagagtag
     3541 ctactgaaga aaggggccgg aagccccacc aaattccagc ttcaacccct cagaattaag
     3601 tcgacctgca gccaagctta tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     3661 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     3721 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag
     3781 acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     3841 ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag
     3901 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     3961 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     4021 caacccctca ctcggcgcgc cagtcctccg atagactgcg tcgcccgggt acccgtgtat
     4081 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     4141 cctctgagtg attgactacc cgtcagcggg ggtctttcat gggtaacagt ttcttgaagt
     4201 tggagaacaa cattctgagg gtaggagtcg aatattaagt aatcctgact caattagcca
     4261 ctgttttgaa tccacatact ccaatactcc tgaaatagtt cattatggac agcgcagaag
     4321 agctggggag aattaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4381 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     4441 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4501 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4561 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4621 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4681 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4741 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4801 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4861 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4921 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4981 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5041 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5101 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5161 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5221 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5281 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5341 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5401 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5461 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5521 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5581 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5641 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5701 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5761 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5821 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5881 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5941 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6001 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6061 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6121 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6181 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6241 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6301 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6361 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6421 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6481 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6541 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     6601 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6661 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6721 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6781 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     6841 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     6901 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     6961 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     7021 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     7081 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     7141 cacgatgcgt ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt
     7201 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     7261 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaa
//
