LOCUS       Exported                6825 bp ds-DNA     circular SYN 18-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 18, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        113..492
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     647..667
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     693..717
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          808..1380
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1471..3531
                     /codon_start=1
                     /label=GaLV
                     /translation="MVLLPGSMLLTSNLHHLRHQMSPGSWKRLIILLSCVFGGGGTSLQ
                     NKNPHQPMTLTWQVLSQTGDVVWDTKAVQPPWTWWPTLKPDVCALAASLESWDIPGTDV
                     SSSKRVRPPDSDYTAAYKQITWGAIGCSYPRARTRMASSTFYVCPRDGRTLSEARRCGG
                     LESLYCKEWDCETTGTGYWLSKSSKDLITVKWDQNSEWTQKFQQCHQTGWCNPLKIDFT
                     DKGKLSKDWITGKTWGLRFYVSGHPGVQFTIRLKITNMPAVAVGPDLVLVEQGPPRTSL
                     ALPPPLPPREAPPPSLPDSNSTALATSAQTPTVRKTIVTLNTPPPTTGDRLFDLVQGAF
                     LTLNATNPGATESCWLCLAMGPPYYEAIASSGEVAYSTDLDRCRWGTQGKLTLTEVSGH
                     GLCIGKVPFTHQHLCNQTLSINSSGDHQYLLPSNHSWWACSTGLTPCLSTSVFNQTRDF
                     CIQVQLIPRIYYYPEEVLLQAYDNSHPRTKREAVSLTLAVLLGLGITAGIGTGSTALIK
                     GPIDLQQGLTSLQIAIDADLRALQDSVSKLEDSLTSLSEVVLQNRRGLDLLFLKEGGLC
                     AALKEECCFYIDHSGAVRDSMKKLKEKLDKRQLERQKSQNWYEGWFNNSPWFTTLISTI
                     MGPLIVLLLILLFGPCILNRLVQFVKDRISVVQALVLTQQYHQLKPIEYEP"
     primer_bind     complement(3546..3565)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_site      3611..3666
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(3665..3684)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4024..4040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4024..4040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4037..4059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4048..4064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4072..4102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4117..4138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4255..4272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4426..5014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4506..4525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5185..6045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5808..5827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6046..6150)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6176..6631)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6313..6334)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6525..6544
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6758..6780
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6772..6789
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6773..6789
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6799..6818
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6799..6817
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac gttgtatcca
       61 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      721 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      781 cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      841 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      901 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      961 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     1021 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     1081 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     1141 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     1201 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac cctggtcatc
     1261 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     1321 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc tttcctacag
     1381 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccgcggg
     1441 cggccgctct agaggatccg ctagactgac atggtattgc tgcctgggtc catgcttctc
     1501 acctcaaacc tgcaccacct tcggcaccag atgagtcctg ggagctggaa aagactgatc
     1561 atcctcttaa gctgcgtatt cggcggcggc gggacgagtc tgcaaaataa gaacccccac
     1621 cagcccatga ccctcacttg gcaggtactg tcccaaactg gagacgttgt ctgggataca
     1681 aaggcagtcc agcccccttg gacttggtgg cccacactta aacctgatgt atgtgccttg
     1741 gcggctagtc ttgagtcctg ggatatcccg ggaaccgatg tctcgtcctc taaacgagtc
     1801 agacctccgg actcagacta tactgccgct tataagcaaa tcacctgggg agccataggg
     1861 tgcagctacc ctcgggctag gactagaatg gcaagctcta ccttctacgt atgtccccgg
     1921 gatggccgga ccctttcaga agctagaagg tgcggggggc tagaatccct atactgtaaa
     1981 gaatgggatt gtgagaccac ggggaccggt tattggctat ctaaatcctc aaaagacctc
     2041 ataactgtaa aatgggacca aaatagcgaa tggactcaaa aatttcaaca gtgtcaccag
     2101 accggctggt gtaaccccct taaaatagat ttcacagaca aaggaaaatt atccaaggac
     2161 tggataacgg gaaaaacctg gggattaaga ttctatgtgt ctggacatcc aggcgtacag
     2221 ttcaccattc gcttaaaaat caccaacatg ccagctgtgg cagtaggtcc tgacctcgtc
     2281 cttgtggaac aaggacctcc tagaacgtcc ctcgctctcc cacctcctct tcccccaagg
     2341 gaagcgccac cgccatctct ccccgactct aactccacag ccctggcgac tagtgcacaa
     2401 actcccacgg tgagaaaaac aattgttacc ctaaacactc cgcctcccac cacaggcgac
     2461 agactttttg atcttgtgca gggggccttc ctaaccttaa atgctaccaa cccaggggcc
     2521 actgagtctt gctggctttg tttggccatg ggcccccctt attatgaagc aatagcctca
     2581 tcaggagagg tcgcctactc caccgacctt gaccggtgcc gctgggggac ccaaggaaag
     2641 ctcaccctca ctgaggtctc aggacacggg ttgtgcatag gaaaggtgcc ctttacccat
     2701 cagcatctct gcaatcagac cctatccatc aattcctccg gagaccatca gtatctgctc
     2761 ccctccaacc atagctggtg ggcttgcagc actggcctca ccccttgcct ctccacctca
     2821 gtttttaatc agactagaga tttctgtatc caggtccagc tgattcctcg catctattac
     2881 tatcctgaag aagttttgtt acaggcctat gacaattctc accccaggac taaaagagag
     2941 gctgtctcac ttaccctagc tgttttactg gggttgggaa tcacggcggg aataggtact
     3001 ggttcaactg ccttaattaa aggacctata gacctccagc aaggcctgac aagcctccag
     3061 atcgccatag atgctgacct ccgggccctc caagactcag tcagcaagtt agaggactca
     3121 ctgacttccc tgtccgaggt agtgctccaa aataggagag gccttgactt gctgtttcta
     3181 aaagaaggtg gcctctgtgc ggccctaaag gaagagtgct gtttttacat agaccactca
     3241 ggtgcagtac gggactccat gaaaaaactc aaagaaaaac tggataaaag acagttagag
     3301 cgccagaaaa gccaaaactg gtatgaagga tggttcaata actccccttg gtttaccacc
     3361 ttgatatcta ccattatggg acccctcatt gtactcctat tgattttgct cttcggaccc
     3421 tgcattctta atcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     3481 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gctcgagggc
     3541 ccagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     3601 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     3661 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     3721 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     3781 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     3841 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     3901 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     3961 gtcatagctg tccctcttct cttatggaga tccctcgacg gatcggccgc aattcgtaat
     4021 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     6181 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6241 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6301 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6361 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6421 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6481 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6541 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     6601 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     6661 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6721 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6781 acggccagtg agcgcgcgta atacgactca ctatagggcg aattg
//
