LOCUS       Exported                9117 bp ds-DNA     circular SYN 12-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Research Software Core
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 12, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the 
                     presenceof cAMP."
     promoter        91..121
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     188..208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        190..208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        236..462
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             463..643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virustype
                     1"
     misc_feature    1308..1541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     enhancer        2049..2352
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2353..2556
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate earlypromoter"
     primer_bind     2507..2527
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2553..2577
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2673..4859
                     /label=DCLK1
                     /note="DCLK1"
     CDS             4860..4901
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4938..4958)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4943..5272
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5123..5258
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5320..5367
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5386..5784
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5871..6104
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6176..6297
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6213..6232)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6267..6286
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6337..6472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6492..6511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6493..6511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6521..6538)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 
                     (-21)Forward"
     primer_bind     complement(6521..6537)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6530..6552)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6679..7134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; 
                     arrowindicates direction of (+) strand synthesis"
     primer_bind     complement(6766..6785)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6976..6997
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7160..7264
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7265..8125
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7483..7502)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8296..8884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8785..8804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9038..9055
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      241 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      301 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      361 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      421 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1201 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1261 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1321 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1381 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1441 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1501 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1561 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1621 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1681 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1741 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1801 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1861 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1921 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1981 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ataagcttgg
     2041 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2101 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2161 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2221 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2281 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2341 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2401 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2461 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2521 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2581 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatccac
     2641 tagtccagtg tggtggaatt gatcccttca ccatgtcctt cggcagagac atggagctgg
     2701 agcacttcga cgagcgggat aaggcgcaga gatacagccg agggtcgcgg gtgaacggcc
     2761 tgccgagccc gacgcacagc gcccactgca gcttctaccg cacccgcacg ctgcagacgc
     2821 tcagctccga gaagaaggcc aagaaagttc gtttctatcg aaacggagat cgatacttca
     2881 aagggattgt gtatgccatc tccccagacc ggttccgatc ttttgaggcc ctgctggctg
     2941 atttgacccg aactctgtcg gataacgtga atttgcccca gggagtgaga acaatctaca
     3001 ccattgatgg gctcaagaag atttccagcc tggaccaact ggtggaagga gagagttatg
     3061 tatgtggctc catagagccc ttcaagaaac tggagtacac caagaatgtg aaccccaact
     3121 ggtcggtgaa cgtcaagacc acctcggctt ctcgggcagt gtcttcactg gccactgcca
     3181 aaggaagccc ttcagaggtg cgagagaata aggatttcat tcggcccaag ctggtcacca
     3241 tcatcagaag tggcgtgaag ccacggaaag ctgtcaggat tctgctgaac aagaaaacgg
     3301 ctcattcctt tgagcaggtc ctcaccgata tcaccgatgc catcaagctg gactcgggag
     3361 tggtgaaacg cctgtacacg ttggatggga aacaggtgat gtgccttcag gacttttttg
     3421 gtgatgatga catttttatt gcatgtggac cggagaagtt ccgttaccag gatgatttct
     3481 tgctagatga aagtgaatgt cgagtggtaa agtccacttc ttacaccaaa atagcttcat
     3541 catcccgcag gagcaccacc aagagcccag gaccgtccag gcgtagcaag tcccctgcct
     3601 ccaccagctc agttaatgga acccctggta gtcagctctc tactccgcgc tcaggcaagt
     3661 cgccaagccc atcacccacc agcccaggaa gcctgcggaa gcagaggagc tctcagcatg
     3721 gcggctcctc tacgtcactt gcgtccacca aagtctgcag ctcgatggat gagaacgatg
     3781 gccctggaga agaagtgtcg gaggaaggct tccagattcc agctacaata acagaacgat
     3841 ataaagtcgg aagaacaata ggagatggaa attttgctgt tgtcaaggaa tgtgtagaaa
     3901 gatcgactgc tagagagtat gctctgaaaa ttatcaagaa aagcaaatgt cgaggcaaag
     3961 agcacatgat ccagaatgaa gtgtctattt taagaagagt gaagcatccc aatatcgttc
     4021 ttctgattga ggagatggat gtgccaactg aactgtatct tgtcatggaa ttagtaaagg
     4081 ggggagacct ttttgatgcc attacttcca ctaacaaata caccgagaga gacgccagtg
     4141 ggatgctgta caacctagcc agcgccatca aatacctgca tagcctgaac atcgtccacc
     4201 gtgatatcaa gccagagaac ctgctggtgt atgagcacca agatggcagc aaatcactga
     4261 agctgggtga ctttggactg gccaccattg tagacggccc cctgtacaca gtctgtggca
     4321 ccccaacata cgtggctcca gaaatcattg cagagactgg atacggcctc aaggtggaca
     4381 tctgggcagc aggtgtaatc acttatatcc tgctgtgtgg tttccctcca ttccgtggaa
     4441 gtggtgatga ccaggaggtg ctttttgatc agattttgat ggggcaggtg gactttcctt
     4501 ctccatactg ggataatgtt tccgattctg caaaggagct cattaccatg atgctgttgg
     4561 tcgatgtaga tcagcgattt tctgctgttc aagtacttga gcatccctgg gttaatgatg
     4621 atggcctccc agaaaatgaa catcagctgt cagtagctgg aaagataaag aagcatttca
     4681 acacaggccc caagccgaat agcacagcag ctggagtttc tgtcatagca ctggaccacg
     4741 ggtttaccat caagagatca gggtctttgg actactacca gcaaccagga atgtattgga
     4801 taagaccacc gctcttgata aggagaggca ggttttccga cgaagacgca accaggatgg
     4861 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag
     4921 tttggaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5101 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     5221 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     5281 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     5341 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg
     5401 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     5461 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     5521 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     5581 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     5641 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     5701 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     5761 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac
     5821 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5881 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5941 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6001 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6061 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6121 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6181 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6241 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6301 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     6481 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     6541 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     6601 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     6661 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6721 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6781 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6841 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6901 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6961 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7021 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7081 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7141 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7261 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7321 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7381 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7441 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7501 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7561 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7621 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7681 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7741 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7801 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7861 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7921 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7981 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8041 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8101 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8161 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8221 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8941 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9001 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9061 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatg
//
