LOCUS       plRL118_[Addgene_#16363 6035 bp ds-DNA     circular     17-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        158..230
                     /label="MOP"
     misc_feature    231..236
                     /label="SD"
     gene            244..1179
                     /label="Mtb codon opt Renilla luciferase"
     rep_origin      1607..2226
                     /label="oriE"
     gene            2460..3653
                     /label="Giles Int"
     misc_feature    3654..3947
                     /label="attP"
     misc_feature    4375..4681
                     /label="f1 origin"
     terminator      5210..5252
                     /label="rrnB_T1 term"
     gene            complement(5301..5870)
                     /label="NatR"
     terminator      5993..6035
                     /label="rrnB_T1 term"
ORIGIN
        1 TTTGATGCCT GGCAGTCGAT CGTACGCTAG TTAACTACGT CGACATCGAT CCATATGACT
       61 AGTAGATCCT CTGCCAGGCA TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT
      121 GCGTTTCTAC AAACTCTTCC TGTCGTCATA TCTAGACCCC AGGCTTGACA CTTTATGCTT
      181 CCGGCTCGTA TAATGTGTGG AATTGTGAGC GCTCACAATT CGGATCCAGC AGGAGGTATC
      241 TCCATGGCGA GCAAGGTCTA CGACCCCGAG CAACGTAAGC GCATGATCAC CGGCCCCCAA
      301 TGGTGGGCGC GGTGCAAGCA GATGAATGTG CTGGACTCGT TCATCAATTA CTATGACTCC
      361 GAAAAACACG CCGAAAACGC CGTGATTTTC CTGCACGGCA ATGCCGCCTC GTCGTACCTC
      421 TGGCGGCACG TGGTGCCCCA CATCGAGCCC GTGGCCCGGT GCATCATCCC GGACCTGATC
      481 GGCATGGGCA AGTCGGGCAA GTCGGGTAAC GGCAGCTACC GCTTGCTGGA CCACTATAAA
      541 TACTTGACCG CCTGGTTTGA ATTGCTGAAC TTGCCGAAAA AGATCATTTT CGTGGGCCAT
      601 GACTGGGGTG CCTGCCTGGC CTTTCACTAT AGCTACGAGC ACCAAGACAA GATTAAGGCG
      661 ATCGTCCACG CGGAGAGCGT GGTGGATGTG ATCGAGAGCT GGGACGAGTG GCCGGACATC
      721 GAGGAGGACA TTGCCCTGAT CAAAAGCGAA GAAGGCGAGA AGATGGTCCT GGAGAACAAC
      781 TTCTTCGTGG AGACCATGCT GCCATCGAAG ATCATGCGCA AACTCGAACC GGAGGAGTTC
      841 GCCGCGTACC TCGAACCCTT TAAGGAGAAA GGGGAGGTGC GCCGGCCGAC CTTGTCGTGG
      901 CCCCGCGAAA TCCCGCTGGT CAAGGGCGGC AAGCCGGACG TCGTCCAGAT CGTGCGGAAC
      961 TACAACGCGT ATCTCCGGGC GTCGGACGAC CTGCCCAAGA TGTTCATTGA AAGCGATCCA
     1021 GGATTTTTCT CGAACGCCAT CGTGGAGGGT GCGAAGAAGT TCCCGAACAC CGAGTTCGTC
     1081 AAAGTCAAGG GACTGCACTT CAGCCAGGAG GACGCCCCCG ACGAGATGGG GAAATATATC
     1141 AAGTCGTTCG TGGAGCGCGT CTTGAAGAAC GAACAGTAGT TCCCTATAGT GAGTCGTATT
     1201 AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA
     1261 TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA
     1321 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
     1381 GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT
     1441 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT
     1501 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA
     1561 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT
     1621 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT
     1681 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
     1741 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA
     1801 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT
     1861 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA
     1921 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG
     1981 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
     2041 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA
     2101 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG
     2161 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT
     2221 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG
     2281 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT GGAATGATGA TCGCCGCGGT
     2341 GACACAATCG GCGTACGCGG ACGCCCGGAC GTGACCGGAG GCGACCGTAC ACCTATCCCG
     2401 ACCGGGCACT ATTCGGCGTG GATCCCCACG CACCCGGCGA CCCCCGAGAG GACACCGCAA
     2461 TGGGGCGACG CGGCAACCGC GAAGGCACAT ACCGGCACCG GCCGGACGGG CGCTGGGAGG
     2521 CCCGGTTGCT CTATGACGAC CCGGTCACCG GTGAACGCCG CCGGGCATCG TTCTACGGCA
     2581 AGACGAAGAC GGCGGTACGG GCCAAAATGC GCGAAGCTAT CGACCGCCTC GACGCCGACG
     2641 CCCCGGTGCG TGACGCCGCG GTGACCGTGG CCGATTGGAT GCGCCGGTGG TGCACCACCA
     2701 CCCTGTTGGT CTCCGGCCGC GCCGACAGCA CCAAATCGTT GTACGGCACA CTGTCGCGCC
     2761 AATACGTCGA ACCCGCCCCG TTCGGCGCGA TCCGGTTGGA CCGCCTGCGC ACATCCGATG
     2821 TCGAGGCGTT GGTGCTGCGC CTTCAACAGG CCACCCGCCC GGGCCGCGAC GGGACGCCGG
     2881 TGCGCGCGGT CGCCGACAGC ACGATCCGGT CGACCTACAC CGTGTTGCGC CAGGCGCTCG
     2941 ACGGCGCGGT GCGCGACGGC CTGATGGCGC GCAACCCTGC GGCGGCCATG AAGCGGCCCG
     3001 GGTTGTCGCG CCCGGAAGTC CGGCGGATCA ACGACGCGCA GTTGGCCGAC CTGTTGGCCG
     3061 CCACCGCCGG ATCCCGCTAC CACCGCGCCC TGTTGCTGAT CGCGGCGACC GGACTGCGCG
     3121 CCGGGGAGGC GTTGGCCCTT CGGTGGGACG CCGTCGACCT CGACGCCGGG TCGCTGCGCG
     3181 TGGTGGCCAC CGTCGGCCGC GTCGAGCGTC GCCTGCGCCT CACGACGCCG AAGACGGAAC
     3241 GATCCCGCCG GACGGTGCCG TTGACGCCGG ACATGGTGTC GATGCTGCGT CGGCACCGCG
     3301 CCGACCAGTT AGCTGAACGC CTGCGCGCGG CCAACGTGTG GGAGGACCAC GGGTTGGTGT
     3361 TCCCGTCGGC GTTCGGCCGC CCGGTGGATC CGCGCAACCT GTTGCGGGTG GTGGAGCAGG
     3421 CCGCCGCGCA GGTTGGCCTC GAGGGCGTCG GCGTGCACAC CCTGCGTCAC GCCGCGGCGG
     3481 TGGCGATGCT CGAGGCGGGC ATCCACATCC GCGCGGTCGC CGACATCCTC GGGCATTCGT
     3541 CGGTGGCGGT CACGGGGGAC ATCTACGGCC ACACGTCCGA CGCGACCGCG CGGGCCGCCG
     3601 TCGACGTGTT GTCGGCGCGC CTGCGGATCG CGGACGGCAG CGGACAGTCT TAGCGAGCTC
     3661 GACGGACGCG GACGGACGCC GGAGCAATTG GGGTCAAAAT TGGGGTCAAA CAGAAAAAGG
     3721 GGCGGCTTCC CGTCTCCGGA AAACCGCCCC TGATCTGCGT CGGGCTGACA GGATTTGAAC
     3781 CTGCGACCCC TTGACCCCCA GTCATTCAAA AGGCATCCGC GTGGATCCGC CCGGGTACCG
     3841 AATCCGCAGG TCACGCCCGG TTGCGCACAA TGGCGGACAT CGGCGAATCG CCCCGAACCC
     3901 CGATGCAATT GGGGTCAAAA TTGGGGTCAA ACCGGCGGGG GTGACCGCTT AACTCTGCGA
     3961 ATACGTGTAG GTCGGCGTCA CGTCGACCTG GCCCGGCCCC GCGGAGTTGA TCGTCAAAAA
     4021 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     4081 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     4141 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     4201 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     4261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
     4321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA
     4381 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG
     4441 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA
     4501 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC
     4561 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT
     4621 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA
     4681 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC
     4741 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA
     4801 ACGTTTACAA TTTCCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
     4861 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT
     4921 TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCGAG
     4981 CTCGGTACCC GGGGATCCGA TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     5041 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     5101 ATAGTTGCCT GACTCCCCGA CGTGGCCGAC CAGCCCGTCC TGcgcaacgt tcaaatccgc
     5161 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     5221 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     5281 catggggaga gatgcatcta tcaggggcag ggcatgctca tgtagagcgc ctgctcgccg
     5341 tccgaggcgg tgccgtcgta cagggcggtg tccaggccgc agagggtgaa ccccatccgc
     5401 cggtacgcgt ggatcgccgg tgcgttgacg ttggtgacct ccagccagag gtgcccggcg
     5461 ccccgctcgc gggcgaactc cgtcgcgagc cccatcaacg cgcgcccgac cccgtgcccc
     5521 cggtgctccg gggcgacctc gatgtcctcg acggtcagcc ggcggttcca gccggagtac
     5581 gagacgacca cgaagcccgc caggtcgccg tcgtccccgt acgcgacgaa cgtccgggag
     5641 tccgggtcgc cgtcctcccc ggcgtccgat tcgtcgtccg attcgtcgtc ggggaacacc
     5701 ttggtcaggg gcgggtccac cggcacctcc cgcagggtga agccgtcccc ggtggcggtg
     5761 acgcggaaga cggtgtcggt ggtgaaggac ccatccagtg cctcgatggc ctcggcgtcc
     5821 cccgggacac tggtgcggta ccggtaagcc gtgtcgtcaa gagtggtcat aggtatatct
     5881 ccttcgcatg cctcccagat tatatcactc gagctagctg gtgtcaaggc gcgcCAGGAC
     5941 ACCGCCCCCG GCGCCTGACG CCCCTCTAGC TGATCACCGC GGCCATGATG GCATAAAACG
     6001 AAAGGCCCAG TCTTTCGACT GAGCCTTTCG TTTTA
//