LOCUS       pEGFP-N1                6824 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
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COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                   13.9386 0 -10 3.34684 0.799245 #228#) 
COMMENT                             #230=(CScratch 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2175)" "@N (@S)" 5 13.4592 
COMMENT                                   -5.22699 0 -10 3.62158 0.799245 #230#) 
COMMENT                             #232=(CScratch 
COMMENT                                   (CWidget 3 (1 #6# 3) 1 2 0 0 #140# 82417488 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2769)" "@N (@S)" 5 9.83833 
COMMENT                                   -12.1963 0 -10 3.87134 0.799245 #232#) 
COMMENT                             #234=(CScratch 
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COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (4578)" "@N (@S)" 5 -13.3145 
COMMENT                                   -6.96932 0 -10 3.87134 0.799245 #234#) 
COMMENT                             #236=(CScratch 
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COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (5281)" "@N (@S)" 5 -14.243 
COMMENT                                   1.74233 0 -10 3.69651 0.799245 #236#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #238=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -242 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #239=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -456 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList #240=(XLATFRAGMENT 624 1368 0 1 625 4106)) 
COMMENT        (CObList #241=(AnlzXLATItem 624 4730 1 624 4730)) 1 (CObList)) (CObList) 
COMMENT       0 (CStringList) 10 2081718712 (CObList)))
FEATURES             Location/Qualifiers
     CDS             4720..5514
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    733..2748
                     /vntifkey="21"
                     /label=TfR-Extracellular\domain
     misc_feature    652..732
                     /vntifkey="21"
                     /label=TfR-TMD
     misc_feature    2770..3489
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    625..651
                     /vntifkey="21"
                     /label=ST-Cyto
BASE COUNT     1731 a      1715 c      1738 g      1640 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatgatt cacaccaacc tgaagaaaaa gtgtagtgga 
      661 agtatctgct atgggactat tgctgtgatc gtctttttct tgattggatt tatgattggc 
      721 tacttgggct attgtaaagg ggtagaacca aaaactgagt gtgagagact ggcaggaacc 
      781 gagtctccag tgagggagga gccaggagag gacttccctg cagcacgtcg cttatattgg 
      841 gatgacctga agagaaagtt gtcggagaaa ctggacagca cagacttcac cagcaccatc 
      901 aagctgctga atgaaaattc atatgtccct cgtgaggctg gatctcaaaa agatgaaaat 
      961 cttgcgttgt atgttgaaaa tcaatttcgt gaatttaaac tcagcaaagt ctggcgtgat 
     1021 caacattttg ttaagattca ggtcaaagac agcgctcaaa actcggtgat catagttgat 
     1081 aagaacggta gacttgttta cctggtggag aatcctgggg gttatgtggc gtatagtaag 
     1141 gctgcaacag ttactggtaa actggtccat gctaattttg gtactaaaaa agattttgag 
     1201 gatttataca ctcctgtgaa tggatctata gtgattgtca gagcagggaa aatcaccttt 
     1261 gcagaaaagg ttgcaaatgc tgaaagctta aatgcaattg gtgtgttgat atacatggac 
     1321 cagactaaat ttcccattgt taacgcagaa ctttcattct ttggacatgc tcatctgggg 
     1381 acaggtgacc cttacacacc tggattccct tccttcaatc acactcagtt tccaccatct 
     1441 cggtcatcag gattgcctaa tatacctgtc cagacaatct ccagagctgc tgcagaaaag 
     1501 ctgtttggga atatggaagg agactgtccc tctgactgga aaacagactc tacatgtagg 
     1561 atggtaacct cagaaagcaa gaatgtgaag ctcactgtga gcaatgtgct gaaagagata 
     1621 aaaattctta acatctttgg agttattaaa ggctttgtag aaccagatca ctatgttgta 
     1681 gttggggccc agagagatgc atggggccct ggagctgcaa aatccggtgt aggcacagct 
     1741 ctcctattga aacttgccca gatgttctca gatatggtct taaaagatgg gtttcagccc 
     1801 agcagaagca ttatctttgc cagttggagt gctggagact ttggatcggt tggtgccact 
     1861 gaatggctag agggatacct ttcgtccctg catttaaagg ctttcactta tattaatctg 
     1921 gataaagcgg ttcttggtac cagcaacttc aaggtttctg ccagcccact gttgtatacg 
     1981 cttattgaga aaacaatgca aaatgtgaag catccggtta ctgggcaatt tctatatcag 
     2041 gacagcaact gggccagcaa agttgagaaa ctcactttag acaatgctgc tttccctttc 
     2101 cttgcatatt ctggaatccc agcagtttct ttctgttttt gcgaggacac agattatcct 
     2161 tatttgggta ccaccatgga cacctataag gaactgattg agaggattcc tgagttgaac 
     2221 aaagtggcac gagcagctgc agaggtcgct ggtcagttcg tgattaaact aacccatgat 
     2281 gttgaattga acctggacta tgagaggtac aacagccaac tgctttcatt tgtgagggat 
     2341 ctgaaccaat acagagcaga cataaaggaa atgggcctga gtttacagtg gctgtattct 
     2401 gctcgtggag acttcttccg tgctacttcc agactaacaa cagatttcgg gaatgctgag 
     2461 aaaacagaca gatttgtcat gaagaaactc aatgatcgtg tcatgagagt ggagtatcac 
     2521 ttcctctctc cctacgtatc tccaaaagag tctcctttcc gacatgtctt ctggggctcc 
     2581 ggctctcaca cgctgccagc tttactggag aacttgaaac tgcgtaaaca aaataacggt 
     2641 gcttttaatg aaacgctgtt cagaaaccag ttggctctag ctacttggac tattcaggga 
     2701 gctgcaaatg ccctctctgg tgacgtttgg gacattgaca atgagtttcg ggatccaccg 
     2761 gtcgccacca tggtgtccaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 
     2821 gagctggacg gcgacgtaaa cggccacaag ttctccgtgc ggggcgaggg cgagggcgat 
     2881 gccaccaacg gcaagctgac cctgaagttc atcagcacca ccggcaagct gcccgtgccc 
     2941 tggcccaccc tcgtgaccac cctgacctac ggcgtgcaga gcttctcccg ctaccccgac 
     3001 cacatgaagc gccacgactt cttcaagagc gccatgcccg aaggctacgt ccaggagcgc 
     3061 accatctcct tcaaggacga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc 
     3121 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 
     3181 ctggggcaca agctggagta caacttcaac tcccacaacg tctatatcac cgccgacaag 
     3241 cagaagaacg gcatcaaggc caacttcaag atccgccaca acgtggagga cggctccgtg 
     3301 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 
     3361 gacaaccact acctgtccac ccagtccaag ctgtccaaag accccaacga gaagcgcgat 
     3421 cacatggtcc ttctggaatt cgtgaccgcc gccgggatca ctcacggcat ggacgagctg 
     3481 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt 
     3541 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa 
     3601 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     3661 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     3721 tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat 
     3781 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa 
     3841 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta 
     3901 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca 
     3961 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat 
     4021 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg 
     4081 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc 
     4141 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt 
     4201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 
     4261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 
     4321 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt 
     4381 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     4441 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     4501 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 
     4561 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 
     4621 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 
     4681 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 
     4741 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 
     4801 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 
     4861 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 
     4921 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 
     4981 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 
     5041 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 
     5101 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 
     5161 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 
     5221 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 
     5281 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 
     5341 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 
     5401 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 
     5461 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga 
     5521 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 
     5581 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 
     5641 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa 
     5701 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac 
     5761 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct 
     5821 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct 
     5881 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg 
     5941 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc 
     6001 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     6061 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     6121 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     6181 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     6241 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 
     6301 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     6361 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     6421 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     6481 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     6541 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     6601 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     6661 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     6721 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 
     6781 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat 
//
