LOCUS pEGFP-N1 6008 bp DNA circular SYN 23-MAR-2007
DEFINITION Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION U55762
VERSION U55762.1 GI:1377911
SOURCE Cloning vector pEGFP-N1.
ORGANISM Cloning vector pEGFP-N1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4733)
AUTHORS Cormack,B.P., Valdivia,R.H. and Falkow,S.
TITLE FACS-optimized mutants of the green fluorescent protein (GFP)
JOURNAL Gene 173 (1), 33-38 (1996)
REFERENCE 2 (bases 1 to 4733)
AUTHORS Kitts,P.A.
TITLE pEGFP-N1 complete sequence
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4733)
AUTHORS Kitts,P.A.
TITLE Direct Submission
JOURNAL Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
order call (415) 424-8222 or (800) 662-2566, extension 1.
International customers, please contact your local distributor.
For technical information, call (415) 424-8222 or (800) 662-2566,
extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH. If you suspect there is an
error in this sequence, please contact CLONTECH's Technical
Service Department at (415) 424-8222 or (800) 662-2566, extension
3 or E-mail TECH@CLONTECH.COM.
COMMENT This file is created by Vector NTI
http://www.invitrogen.com/
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COMMENT 0 (CStringList) 10 2081718712 (CObList)))
FEATURES Location/Qualifiers
CDS 3904..4698
/codon_start=1
/transl_table=11
/product="neomycin phosphotransferase"
/protein_id="AAB02575.1"
/db_xref="GI:1377913"
/vntifkey="4"
/label=CDS_1
misc_feature 1159..1161
/vntifkey="21"
/label=N149\N-glycosylation
misc_feature 1195..1197
/vntifkey="21"
/label=N161\N-glycosylation
misc_feature 519..539
/vntifkey="21"
/label=CMV-F
misc_feature 793..1932
/vntifkey="21"
/label=ST6\luminal
misc_feature 625..711
/vntifkey="21"
/label=TNFa-cyto
misc_feature 712..792
/vntifkey="21"
/label=TNFa-TMD
misc_feature 1954..2673
/vntifkey="21"
/label=moxGFP
BASE COUNT 1479 a 1629 c 1544 g 1356 t
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgaggc caccatgtct accgagtcta tgattaggga cgtggaactg
661 gctgaggagg cactgcccaa aaaaaccggc ggaccacagg gctctaggag atgtctgttt
721 ctgtctctgt tctcttttct catcgtggct ggcgctacaa cactcttctg tctgctccat
781 ttcggcgtga ttaaggaaaa gaagaaaggg agttactatg attcctttaa attgcaaacc
841 aaggaattcc aggtgttaaa gagtctgggg aaattggcca tggggtctga ttcccagtct
901 gtatcctcaa gcagcaccca ggacccccac aggggccgcc agaccctcgg cagtctcaga
961 ggcctagcca aggccaaacc agaggcctcc ttccaggtgt ggaacaagga cagctcttcc
1021 aaaaacctta tccctaggct gcaaaagatc tggaagaatt acctaagcat gaacaagtac
1081 aaagtgtcct acaaggggcc aggaccaggc atcaagttca gtgcagaggc cctgcgctgc
1141 cacctccggg accatgtgaa tgtatccatg gtagaggtca cagattttcc cttcaatacc
1201 tctgaatggg agggttatct gcccaaggag agcattagga ccaaggctgg gccttggggc
1261 aggtgtgctg ttgtgtcgtc agcgggatct ctgaagtcct cccaactagg cagagaaatc
1321 gatgatcatg acgcagtcct gaggtttaat ggggcaccca cagccaactt ccaacaagat
1381 gtgggcacaa aaactaccat tcgcctgatg aactctcagt tggttaccac agagaagcgc
1441 ttcctcaaag acagtttgta caatgaagga atcctaattg tatgggaccc atctgtatac
1501 cactcagata tcccaaagtg gtaccagaat ccggattata atttctttaa caactacaag
1561 acttatcgta agctgcaccc caatcagccc ttttacatcc tcaagcccca gatgccttgg
1621 gagctatggg acattcttca agaaatctcc ccagaagaga ttcagccaaa ccccccatcc
1681 tctgggatgc ttggtatcat catcatgatg acgctgtgtg accaggtgga tatttatgag
1741 ttcctcccat ccaagcgcaa gactgacgtg tgctactact accagaagtt cttcgatagt
1801 gcctgcacga tgggtgccta ccacccgctg ctctatgaga agaatttggt gaagcatctc
1861 aaccagggca cagatgagga catctacctg cttggaaaag ccacactgcc tggcttccgg
1921 accattcact gccgggatcc accggtcgcc accatggtgt ccaagggcga ggagctgttc
1981 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttctcc
2041 gtgcggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatcagc
2101 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
2161 cagagcttct cccgctaccc cgaccacatg aagcgccacg acttcttcaa gagcgccatg
2221 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcac ctacaagacc
2281 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
2341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caactcccac
2401 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
2461 cacaacgtgg aggacggctc cgtgcagctc gccgaccact accagcagaa cacccccatc
2521 ggcgacggcc ccgtgctgct gcccgacaac cactacctgt ccacccagtc caagctgtcc
2581 aaagacccca acgagaagcg cgatcacatg gtccttctgg aattcgtgac cgccgccggg
2641 atcactcacg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
2701 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2761 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
2821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
2881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
2941 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
3001 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
3061 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
3121 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
3181 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
3241 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
3301 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
3361 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
3421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
3481 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
3541 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3601 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
3661 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
3721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
3781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
3841 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
3901 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
3961 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
4021 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
4081 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
4141 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
4201 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
4261 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
4321 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
4381 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
4441 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
4501 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
4561 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
4621 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
4681 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
4741 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
4801 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
4861 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
4921 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
4981 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
5041 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
5101 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
5161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
5221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
5281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
5341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
5401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
5461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
5521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
5581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
5821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
5881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
5941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
6001 ccatgcat
//