LOCUS pEGFP-N1 6008 bp DNA circular SYN 23-MAR-2007 DEFINITION Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds. ACCESSION U55762 VERSION U55762.1 GI:1377911 SOURCE Cloning vector pEGFP-N1. ORGANISM Cloning vector pEGFP-N1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4733) AUTHORS Cormack,B.P., Valdivia,R.H. and Falkow,S. TITLE FACS-optimized mutants of the green fluorescent protein (GFP) JOURNAL Gene 173 (1), 33-38 (1996) REFERENCE 2 (bases 1 to 4733) AUTHORS Kitts,P.A. TITLE pEGFP-N1 complete sequence JOURNAL Unpublished REFERENCE 3 (bases 1 to 4733) AUTHORS Kitts,P.A. TITLE Direct Submission JOURNAL Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424-8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|441652552| COMMENT VNTDBDATE|441652552| COMMENT LSOWNER| COMMENT VNTNAME|pEGFP-N1| COMMENT VNTAUTHORNAME|LL| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#139# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Nco}I (4465)" "@N (@S)" 5 -14.3801 0 0 COMMENT -10 3.97125 0.799245 #243#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #245=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -385 100) COMMENT (CObjectList) (CObjectList)) COMMENT #246=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1292 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList #247=(XLATFRAGMENT 624 1794 0 1 625 5383)) COMMENT (CObList #248=(AnlzXLATItem 624 6007 1 624 6007)) 1 (CObList)) (CObList) COMMENT 0 (CStringList) 10 2081718712 (CObList))) FEATURES Location/Qualifiers CDS 3904..4698 /codon_start=1 /transl_table=11 /product="neomycin phosphotransferase" /protein_id="AAB02575.1" /db_xref="GI:1377913" /vntifkey="4" /label=CDS_1 misc_feature 1159..1161 /vntifkey="21" /label=N149\N-glycosylation misc_feature 1195..1197 /vntifkey="21" /label=N161\N-glycosylation misc_feature 519..539 /vntifkey="21" /label=CMV-F misc_feature 793..1932 /vntifkey="21" /label=ST6\luminal misc_feature 625..711 /vntifkey="21" /label=TNFa-cyto misc_feature 712..792 /vntifkey="21" /label=TNFa-TMD misc_feature 1954..2673 /vntifkey="21" /label=moxGFP BASE COUNT 1479 a 1629 c 1544 g 1356 t ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgtct accgagtcta tgattaggga cgtggaactg 661 gctgaggagg cactgcccaa aaaaaccggc ggaccacagg gctctaggag atgtctgttt 721 ctgtctctgt tctcttttct catcgtggct ggcgctacaa cactcttctg tctgctccat 781 ttcggcgtga ttaaggaaaa gaagaaaggg agttactatg attcctttaa attgcaaacc 841 aaggaattcc aggtgttaaa gagtctgggg aaattggcca tggggtctga ttcccagtct 901 gtatcctcaa gcagcaccca ggacccccac aggggccgcc agaccctcgg cagtctcaga 961 ggcctagcca aggccaaacc agaggcctcc ttccaggtgt ggaacaagga cagctcttcc 1021 aaaaacctta tccctaggct gcaaaagatc tggaagaatt acctaagcat gaacaagtac 1081 aaagtgtcct acaaggggcc aggaccaggc atcaagttca gtgcagaggc cctgcgctgc 1141 cacctccggg accatgtgaa tgtatccatg gtagaggtca cagattttcc cttcaatacc 1201 tctgaatggg agggttatct gcccaaggag agcattagga ccaaggctgg gccttggggc 1261 aggtgtgctg ttgtgtcgtc agcgggatct ctgaagtcct cccaactagg cagagaaatc 1321 gatgatcatg acgcagtcct gaggtttaat ggggcaccca cagccaactt ccaacaagat 1381 gtgggcacaa aaactaccat tcgcctgatg aactctcagt tggttaccac agagaagcgc 1441 ttcctcaaag acagtttgta caatgaagga atcctaattg tatgggaccc atctgtatac 1501 cactcagata tcccaaagtg gtaccagaat ccggattata atttctttaa caactacaag 1561 acttatcgta agctgcaccc caatcagccc ttttacatcc tcaagcccca gatgccttgg 1621 gagctatggg acattcttca agaaatctcc ccagaagaga ttcagccaaa ccccccatcc 1681 tctgggatgc ttggtatcat catcatgatg acgctgtgtg accaggtgga tatttatgag 1741 ttcctcccat ccaagcgcaa gactgacgtg tgctactact accagaagtt cttcgatagt 1801 gcctgcacga tgggtgccta ccacccgctg ctctatgaga agaatttggt gaagcatctc 1861 aaccagggca cagatgagga catctacctg cttggaaaag ccacactgcc tggcttccgg 1921 accattcact gccgggatcc accggtcgcc accatggtgt ccaagggcga ggagctgttc 1981 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttctcc 2041 gtgcggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatcagc 2101 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 2161 cagagcttct cccgctaccc cgaccacatg aagcgccacg acttcttcaa gagcgccatg 2221 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcac ctacaagacc 2281 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 2341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caactcccac 2401 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc 2461 cacaacgtgg aggacggctc cgtgcagctc gccgaccact accagcagaa cacccccatc 2521 ggcgacggcc ccgtgctgct gcccgacaac cactacctgt ccacccagtc caagctgtcc 2581 aaagacccca acgagaagcg cgatcacatg gtccttctgg aattcgtgac cgccgccggg 2641 atcactcacg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca 2701 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 2761 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 2821 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 2881 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat 2941 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 3001 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 3061 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 3121 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 3181 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 3241 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 3301 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 3361 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 3421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 3481 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 3541 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3601 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 3661 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 3721 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 3781 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 3841 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 3901 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 3961 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 4021 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 4081 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 4141 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 4201 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 4261 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 4321 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 4381 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 4441 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 4501 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 4561 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 4621 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 4681 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 4741 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 4801 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 4861 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 4921 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 4981 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 5041 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 5101 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 5161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 5221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 5281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 5341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 5401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 5461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 5821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 6001 ccatgcat //