LOCUS       pAcGFP1N1(w/o\Nd        5034 bp    DNA     linear        1-DEC-2015
DEFINITION  pAcGFP1-N1.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752179674|
COMMENT     VNTDBDATE|752183982|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAcGFP1N1(w/o NdeI)-ST6Gal1(Residue1-113)|
COMMENT     VNTAUTHORNAME|Demo User|
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     promoter        10..564
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    65..354
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    521..541
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        522..591
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    1913..1932
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(2082..2388)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        2467..2495
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(2561..2581)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2567..2782)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2579..2847
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2746..2823
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2808..2827
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2930..3724
                     /vntifkey="4"
                     /label=NeoR/KanR
     gene            2933..3721
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(3239..3775)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      3899..4168
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      4316..4935
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_marker     233..240
                     /vntifkey="22"
                     /label=NdeI\abolished\site
     misc_feature    627..965
                     /vntifkey="21"
                     /label=ST6Gal1(Residue\1-113)
     misc_feature    654..704
                     /vntifkey="21"
                     /label=TMD\of\ST
     misc_feature    705..965
                     /vntifkey="21"
                     /label=patial\lumenal\domain\of\ST
     misc_feature    981..1700
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    627..653
                     /vntifkey="21"
                     /label=cytosolic\tail\of\ST
BASE COUNT     1226 a      1368 c      1311 g      1129 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatatg 
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc 
      601 taccggactc agatctcgag gccaccatga ttcacaccaa cctgaagaaa aagttcagct 
      661 gctgcgtact agtctttctt ctgtttgcag tcatatgtgt gtggaaggaa aagaagaaag 
      721 ggagttacta tgattccttt aaattgcaaa ccaaggaatt ccaggtgtta aagagtctgg 
      781 ggaaattggc catggggtct gattcccagt ctgtatcctc aagcagcacc caggaccccc 
      841 acaggggccg ccagaccctc ggcagtctca gaggcctagc caaggccaaa ccagaggcct 
      901 ccttccaggt gtggaacaag gacagctctt ccaaaaacct tatccctagg ctgcaaaaga 
      961 tctgggcacc ggtcgccacc atggtgtcca agggcgagga gctgttcacc ggggtggtgc 
     1021 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttctccgtg cggggcgagg 
     1081 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catcagcacc accggcaagc 
     1141 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag agcttctccc 
     1201 gctaccccga ccacatgaag cgccacgact tcttcaagag cgccatgccc gaaggctacg 
     1261 tccaggagcg caccatctcc ttcaaggacg acggcaccta caagacccgc gccgaggtga 
     1321 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 
     1381 acggcaacat cctggggcac aagctggagt acaacttcaa ctcccacaac gtctatatca 
     1441 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacgtggagg 
     1501 acggctccgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 
     1561 tgctgctgcc cgacaaccac tacctgtcca cccagtccaa gctgtccaaa gaccccaacg 
     1621 agaagcgcga tcacatggtc cttctggaat tcgtgaccgc cgccgggatc actcacggca 
     1681 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacattt 
     1741 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 
     1801 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 
     1861 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     1921 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt 
     1981 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 
     2041 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 
     2101 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 
     2161 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 
     2221 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 
     2281 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 
     2341 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 
     2401 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     2461 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     2521 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 
     2581 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 
     2641 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     2701 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     2761 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     2821 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     2881 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 
     2941 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 
     3001 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 
     3061 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 
     3121 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 
     3181 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 
     3241 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 
     3301 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 
     3361 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 
     3421 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 
     3481 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 
     3541 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 
     3601 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 
     3661 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 
     3721 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 
     3781 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 
     3841 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 
     3901 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 
     3961 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 
     4021 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 
     4081 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 
     4141 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 
     4201 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 
     4261 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 
     4321 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 
     4381 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 
     4441 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 
     4501 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 
     4561 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 
     4621 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 
     4681 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 
     4741 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 
     4801 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 
     4861 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 
     4921 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 
     4981 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat 
//
