LOCUS       pEGFP-N1                5918 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
COMMENT     VNTDBDATE|441652552|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|LL|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     CDS             3814..4608
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    1069..1071
                     /vntifkey="21"
                     /label=N149\N-glycosylation
     misc_feature    1105..1107
                     /vntifkey="21"
                     /label=N161\N-glycosylation
     misc_feature    652..702
                     /vntifkey="21"
                     /label=ST\TMD
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    703..1842
                     /vntifkey="21"
                     /label=ST6\luminal\region
     misc_feature    1864..2583
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    625..651
                     /vntifkey="21"
                     /label=TfR-cyto-9AAs
BASE COUNT     1463 a      1604 c      1519 g      1332 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatggcc aatgtcacaa aaccaaaaag gttcagctgc 
      661 tgcgtcctgg tctttcttct gtttgcagtc atctgtgtgt ggaaggaaaa gaagaaaggg 
      721 agttactatg attcctttaa attgcaaacc aaggaattcc aggtgttaaa gagtctgggg 
      781 aaattggcca tggggtctga ttcccagtct gtatcctcaa gcagcaccca ggacccccac 
      841 aggggccgcc agaccctcgg cagtctcaga ggcctagcca aggccaaacc agaggcctcc 
      901 ttccaggtgt ggaacaagga cagctcttcc aaaaacctta tccctaggct gcaaaagatc 
      961 tggaagaatt acctaagcat gaacaagtac aaagtgtcct acaaggggcc aggaccaggc 
     1021 atcaagttca gtgcagaggc cctgcgctgc cacctccggg accatgtgaa tgtatccatg 
     1081 gtagaggtca cagattttcc cttcaatacc tctgaatggg agggttatct gcccaaggag 
     1141 agcattagga ccaaggctgg gccttggggc aggtgtgctg ttgtgtcgtc agcgggatct 
     1201 ctgaagtcct cccaactagg cagagaaatc gatgatcatg acgcagtcct gaggtttaat 
     1261 ggggcaccca cagccaactt ccaacaagat gtgggcacaa aaactaccat tcgcctgatg 
     1321 aactctcagt tggttaccac agagaagcgc ttcctcaaag acagtttgta caatgaagga 
     1381 atcctaattg tatgggaccc atctgtatac cactcagata tcccaaagtg gtaccagaat 
     1441 ccggattata atttctttaa caactacaag acttatcgta agctgcaccc caatcagccc 
     1501 ttttacatcc tcaagcccca gatgccttgg gagctatggg acattcttca agaaatctcc 
     1561 ccagaagaga ttcagccaaa ccccccatcc tctgggatgc ttggtatcat catcatgatg 
     1621 acgctgtgtg accaggtgga tatttatgag ttcctcccat ccaagcgcaa gactgacgtg 
     1681 tgctactact accagaagtt cttcgatagt gcctgcacga tgggtgccta ccacccgctg 
     1741 ctctatgaga agaatttggt gaagcatctc aaccagggca cagatgagga catctacctg 
     1801 cttggaaaag ccacactgcc tggcttccgg accattcact gccgggatcc accggtcgcc 
     1861 accatggtgt ccaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 
     1921 gacggcgacg taaacggcca caagttctcc gtgcggggcg agggcgaggg cgatgccacc 
     1981 aacggcaagc tgaccctgaa gttcatcagc accaccggca agctgcccgt gccctggccc 
     2041 accctcgtga ccaccctgac ctacggcgtg cagagcttct cccgctaccc cgaccacatg 
     2101 aagcgccacg acttcttcaa gagcgccatg cccgaaggct acgtccagga gcgcaccatc 
     2161 tccttcaagg acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 
     2221 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 
     2281 cacaagctgg agtacaactt caactcccac aacgtctata tcaccgccga caagcagaag 
     2341 aacggcatca aggccaactt caagatccgc cacaacgtgg aggacggctc cgtgcagctc 
     2401 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 
     2461 cactacctgt ccacccagtc caagctgtcc aaagacccca acgagaagcg cgatcacatg 
     2521 gtccttctgg aattcgtgac cgccgccggg atcactcacg gcatggacga gctgtacaag 
     2581 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg 
     2641 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 
     2701 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 
     2761 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 
     2821 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 
     2881 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2941 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     3001 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     3061 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     3121 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     3181 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     3241 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 
     3301 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 
     3361 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 
     3421 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 
     3481 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
     3541 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
     3601 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     3661 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     3721 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 
     3781 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 
     3841 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 
     3901 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 
     3961 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 
     4021 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 
     4081 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 
     4141 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 
     4201 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 
     4261 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 
     4321 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 
     4381 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 
     4441 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 
     4501 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 
     4561 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 
     4621 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 
     4681 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 
     4741 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 
     4801 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 
     4861 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 
     4921 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 
     4981 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 
     5041 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 
     5101 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 
     5161 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     5221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     5281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     5341 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     5401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     5461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     5521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     5581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     5641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     5701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     5761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     5821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     5881 cccctgattc tgtggataac cgtattaccg ccatgcat 
//
