LOCUS       pEGFP-N1                6794 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
COMMENT     VNTDBDATE|441652552|
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COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                   "{\\i Nco}I (361)" "@N (@S)" 5 7.13045 15.681 
COMMENT                                   0 -10 3.34684 0.799245 #229#) 
COMMENT                             #231=(CScratch 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (624)" "@N (@S)" 5 10.871 8.71165 
COMMENT                                   0 -10 3.52167 0.799245 #231#) 
COMMENT                             #233=(CScratch 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2145)" "@N (@S)" 5 13.5182 
COMMENT                                   -5.22699 0 -10 3.69651 0.799245 #233#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2739)" "@N (@S)" 5 10.0016 
COMMENT                                   -12.1963 0 -10 3.79641 0.799245 #235#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (4548)" "@N (@S)" 5 -13.2361 
COMMENT                                   -6.96932 0 -10 3.97125 0.799245 #237#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (5251)" "@N (@S)" 5 -14.2445 
COMMENT                                   1.74233 0 -10 3.62158 0.799245 #239#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #241=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -241 100) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList #243=(XLATFRAGMENT 624 1442 0 1 625 4327)) 
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FEATURES             Location/Qualifiers
     CDS             4690..5484
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    703..2718
                     /vntifkey="21"
                     /label=TfR\luminal
     misc_feature    625..651
                     /vntifkey="21"
                     /label=TfR-cyto-9AAs
     misc_feature    2740..3459
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    652..702
                     /vntifkey="21"
                     /label=ST\TMD
BASE COUNT     1720 a      1717 c      1731 g      1626 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatggcc aatgtcacaa aaccaaaaag gttcagctgc 
      661 tgcgtcctgg tctttcttct gtttgcagtc atctgtgtgt ggtgtaaagg ggtagaacca 
      721 aaaactgagt gtgagagact ggcaggaacc gagtctccag tgagggagga gccaggagag 
      781 gacttccctg cagcacgtcg cttatattgg gatgacctga agagaaagtt gtcggagaaa 
      841 ctggacagca cagacttcac cagcaccatc aagctgctga atgaaaattc atatgtccct 
      901 cgtgaggctg gatctcaaaa agatgaaaat cttgcgttgt atgttgaaaa tcaatttcgt 
      961 gaatttaaac tcagcaaagt ctggcgtgat caacattttg ttaagattca ggtcaaagac 
     1021 agcgctcaaa actcggtgat catagttgat aagaacggta gacttgttta cctggtggag 
     1081 aatcctgggg gttatgtggc gtatagtaag gctgcaacag ttactggtaa actggtccat 
     1141 gctaattttg gtactaaaaa agattttgag gatttataca ctcctgtgaa tggatctata 
     1201 gtgattgtca gagcagggaa aatcaccttt gcagaaaagg ttgcaaatgc tgaaagctta 
     1261 aatgcaattg gtgtgttgat atacatggac cagactaaat ttcccattgt taacgcagaa 
     1321 ctttcattct ttggacatgc tcatctgggg acaggtgacc cttacacacc tggattccct 
     1381 tccttcaatc acactcagtt tccaccatct cggtcatcag gattgcctaa tatacctgtc 
     1441 cagacaatct ccagagctgc tgcagaaaag ctgtttggga atatggaagg agactgtccc 
     1501 tctgactgga aaacagactc tacatgtagg atggtaacct cagaaagcaa gaatgtgaag 
     1561 ctcactgtga gcaatgtgct gaaagagata aaaattctta acatctttgg agttattaaa 
     1621 ggctttgtag aaccagatca ctatgttgta gttggggccc agagagatgc atggggccct 
     1681 ggagctgcaa aatccggtgt aggcacagct ctcctattga aacttgccca gatgttctca 
     1741 gatatggtct taaaagatgg gtttcagccc agcagaagca ttatctttgc cagttggagt 
     1801 gctggagact ttggatcggt tggtgccact gaatggctag agggatacct ttcgtccctg 
     1861 catttaaagg ctttcactta tattaatctg gataaagcgg ttcttggtac cagcaacttc 
     1921 aaggtttctg ccagcccact gttgtatacg cttattgaga aaacaatgca aaatgtgaag 
     1981 catccggtta ctgggcaatt tctatatcag gacagcaact gggccagcaa agttgagaaa 
     2041 ctcactttag acaatgctgc tttccctttc cttgcatatt ctggaatccc agcagtttct 
     2101 ttctgttttt gcgaggacac agattatcct tatttgggta ccaccatgga cacctataag 
     2161 gaactgattg agaggattcc tgagttgaac aaagtggcac gagcagctgc agaggtcgct 
     2221 ggtcagttcg tgattaaact aacccatgat gttgaattga acctggacta tgagaggtac 
     2281 aacagccaac tgctttcatt tgtgagggat ctgaaccaat acagagcaga cataaaggaa 
     2341 atgggcctga gtttacagtg gctgtattct gctcgtggag acttcttccg tgctacttcc 
     2401 agactaacaa cagatttcgg gaatgctgag aaaacagaca gatttgtcat gaagaaactc 
     2461 aatgatcgtg tcatgagagt ggagtatcac ttcctctctc cctacgtatc tccaaaagag 
     2521 tctcctttcc gacatgtctt ctggggctcc ggctctcaca cgctgccagc tttactggag 
     2581 aacttgaaac tgcgtaaaca aaataacggt gcttttaatg aaacgctgtt cagaaaccag 
     2641 ttggctctag ctacttggac tattcaggga gctgcaaatg ccctctctgg tgacgtttgg 
     2701 gacattgaca atgagtttcg ggatccaccg gtcgccacca tggtgtccaa gggcgaggag 
     2761 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 
     2821 ttctccgtgc ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc 
     2881 atcagcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac 
     2941 ggcgtgcaga gcttctcccg ctaccccgac cacatgaagc gccacgactt cttcaagagc 
     3001 gccatgcccg aaggctacgt ccaggagcgc accatctcct tcaaggacga cggcacctac 
     3061 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 
     3121 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac 
     3181 tcccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag 
     3241 atccgccaca acgtggagga cggctccgtg cagctcgccg accactacca gcagaacacc 
     3301 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgtccac ccagtccaag 
     3361 ctgtccaaag accccaacga gaagcgcgat cacatggtcc ttctggaatt cgtgaccgcc 
     3421 gccgggatca ctcacggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca 
     3481 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 
     3541 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 
     3601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     3661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc 
     3721 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa 
     3781 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt 
     3841 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 
     3901 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt 
     3961 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga 
     4021 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 
     4081 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg 
     4141 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 
     4201 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 
     4261 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt 
     4321 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 
     4381 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 
     4441 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 
     4501 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     4561 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     4621 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga 
     4681 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag 
     4741 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc 
     4801 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg 
     4861 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc 
     4921 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg 
     4981 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct 
     5041 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 
     5101 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 
     5161 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc 
     5221 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 
     5281 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 
     5341 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 
     5401 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 
     5461 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 
     5521 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 
     5581 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 
     5641 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag 
     5701 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg 
     5761 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc 
     5821 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg 
     5881 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact 
     5941 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     6001 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     6061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     6121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     6181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 
     6241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     6301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     6361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     6421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     6481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     6541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     6601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     6661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     6721 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     6781 ttaccgccat gcat 
//
