LOCUS       pEGFP-N1                5948 bp    DNA     circular SYN 23-MAR-2007
DEFINITION  Cloning vector pEGFP-N1, complete sequence, enhanced green fluorescent protein (egfp) and neomycin phosphotransferase genes, complete cds.
ACCESSION   U55762
VERSION     U55762.1  GI:1377911
SOURCE      Cloning vector pEGFP-N1.
  ORGANISM  Cloning vector pEGFP-N1
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Cormack,B.P., Valdivia,R.H. and Falkow,S.
  TITLE     FACS-optimized mutants of the green fluorescent protein (GFP)
  JOURNAL   Gene 173 (1), 33-38 (1996)
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     pEGFP-N1 complete sequence
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Kitts,P.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-1996) Paul A. Kitts, CLONTECH Laboratories, Inc.,
            1020 East Meadow Circle, Palo Alto, CA 94303-4230, USA
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020
            East  Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an
            order call  (415) 424-8222 or (800) 662-2566, extension 1.
            International customers,  please contact your local distributor.
            For technical information, call  (415) 424-8222 or (800) 662-2566,
            extension 3.
            This sequence has been compiled from information in the sequence
            databases,  published literature and other sources, together with
            partial sequences  obtained by CLONTECH. If you suspect there is an
            error in this sequence,  please contact CLONTECH's Technical
            Service Department at (415) 424-8222 or  (800) 662-2566, extension
            3 or E-mail TECH@CLONTECH.COM.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|441652552|
COMMENT     VNTDBDATE|441652552|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|LL|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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COMMENT                                   "{\\i Eco}RI (2561)" "@N (@S)" 5 8.65295 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (361)" "@N (@S)" 5 7.56552 
COMMENT                                   13.9386 0 -10 3.34684 0.799245 #234#) 
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COMMENT                                   6.96932 0 -10 3.52167 0.799245 #236#) 
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FEATURES             Location/Qualifiers
     CDS             3844..4638
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="AAB02575.1"
                     /db_xref="GI:1377913"
                     /vntifkey="4"
                     /label=CDS_1
     misc_feature    519..539
                     /vntifkey="21"
                     /label=CMV-F
     misc_feature    625..651
                     /vntifkey="21"
                     /label=TfR-cyto-9AAs
     misc_feature    652..732
                     /vntifkey="21"
                     /label=TfR-TMD
     misc_feature    1099..1101
                     /vntifkey="21"
                     /label=N149\N-glycosylation
     misc_feature    1135..1137
                     /vntifkey="21"
                     /label=N161\N-glycosylation
     misc_feature    733..1872
                     /vntifkey="21"
                     /label=ST6\luminal
     misc_feature    1894..2613
                     /vntifkey="21"
                     /label=moxGFP
BASE COUNT     1474 a      1602 c      1527 g      1345 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggactcag atctcgaggc caccatggcc aatgtcacaa aaccaaaaag gtgtagtgga 
      661 agtatctgct atgggactat tgctgtgatc gtctttttct tgattggatt tatgattggc 
      721 tacttgggct ataaggaaaa gaagaaaggg agttactatg attcctttaa attgcaaacc 
      781 aaggaattcc aggtgttaaa gagtctgggg aaattggcca tggggtctga ttcccagtct 
      841 gtatcctcaa gcagcaccca ggacccccac aggggccgcc agaccctcgg cagtctcaga 
      901 ggcctagcca aggccaaacc agaggcctcc ttccaggtgt ggaacaagga cagctcttcc 
      961 aaaaacctta tccctaggct gcaaaagatc tggaagaatt acctaagcat gaacaagtac 
     1021 aaagtgtcct acaaggggcc aggaccaggc atcaagttca gtgcagaggc cctgcgctgc 
     1081 cacctccggg accatgtgaa tgtatccatg gtagaggtca cagattttcc cttcaatacc 
     1141 tctgaatggg agggttatct gcccaaggag agcattagga ccaaggctgg gccttggggc 
     1201 aggtgtgctg ttgtgtcgtc agcgggatct ctgaagtcct cccaactagg cagagaaatc 
     1261 gatgatcatg acgcagtcct gaggtttaat ggggcaccca cagccaactt ccaacaagat 
     1321 gtgggcacaa aaactaccat tcgcctgatg aactctcagt tggttaccac agagaagcgc 
     1381 ttcctcaaag acagtttgta caatgaagga atcctaattg tatgggaccc atctgtatac 
     1441 cactcagata tcccaaagtg gtaccagaat ccggattata atttctttaa caactacaag 
     1501 acttatcgta agctgcaccc caatcagccc ttttacatcc tcaagcccca gatgccttgg 
     1561 gagctatggg acattcttca agaaatctcc ccagaagaga ttcagccaaa ccccccatcc 
     1621 tctgggatgc ttggtatcat catcatgatg acgctgtgtg accaggtgga tatttatgag 
     1681 ttcctcccat ccaagcgcaa gactgacgtg tgctactact accagaagtt cttcgatagt 
     1741 gcctgcacga tgggtgccta ccacccgctg ctctatgaga agaatttggt gaagcatctc 
     1801 aaccagggca cagatgagga catctacctg cttggaaaag ccacactgcc tggcttccgg 
     1861 accattcact gccgggatcc accggtcgcc accatggtgt ccaagggcga ggagctgttc 
     1921 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttctcc 
     1981 gtgcggggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatcagc 
     2041 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 
     2101 cagagcttct cccgctaccc cgaccacatg aagcgccacg acttcttcaa gagcgccatg 
     2161 cccgaaggct acgtccagga gcgcaccatc tccttcaagg acgacggcac ctacaagacc 
     2221 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 
     2281 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caactcccac 
     2341 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc 
     2401 cacaacgtgg aggacggctc cgtgcagctc gccgaccact accagcagaa cacccccatc 
     2461 ggcgacggcc ccgtgctgct gcccgacaac cactacctgt ccacccagtc caagctgtcc 
     2521 aaagacccca acgagaagcg cgatcacatg gtccttctgg aattcgtgac cgccgccggg 
     2581 atcactcacg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca 
     2641 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 
     2701 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 
     2761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 
     2821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat 
     2881 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 
     2941 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 
     3001 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 
     3061 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 
     3121 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 
     3181 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 
     3241 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 
     3301 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 
     3361 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 
     3421 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 
     3481 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     3541 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     3601 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     3661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     3721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 
     3781 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 
     3841 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 
     3901 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 
     3961 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 
     4021 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 
     4081 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 
     4141 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 
     4201 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 
     4261 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 
     4321 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 
     4381 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 
     4441 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 
     4501 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 
     4561 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 
     4621 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 
     4681 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 
     4741 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 
     4801 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 
     4861 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 
     4921 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 
     4981 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 
     5041 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 
     5101 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 
     5161 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 
     5221 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 
     5281 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 
     5341 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 
     5401 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 
     5461 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 
     5521 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 
     5581 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 
     5641 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 
     5701 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 
     5761 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 
     5821 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 
     5881 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 
     5941 ccatgcat 
//
