LOCUS       pAcGFP1N1(w/o\Nd        5043 bp    DNA     linear        1-DEC-2015
DEFINITION  pAcGFP1-N1.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752179674|
COMMENT     VNTDBDATE|752183982|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAcGFP1N1(w/o NdeI)-ST6Gal1(Residue1-113)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Created: 12/01/15 06:47PM" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     promoter        10..564
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    65..354
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    521..541
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        522..591
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    1922..1941
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(2091..2397)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        2476..2504
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(2570..2590)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2576..2791)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2588..2856
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2755..2832
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2817..2836
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2939..3733
                     /vntifkey="4"
                     /label=NeoR/KanR
     gene            2942..3730
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(3248..3784)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      3908..4177
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      4325..4944
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_marker     233..240
                     /vntifkey="22"
                     /label=NdeI\abolished\site
     misc_feature    627..974
                     /vntifkey="21"
                     /label=ST6Gal1(Residue\1-113)
     misc_feature    654..713
                     /vntifkey="21"
                     /label=TMD20
     misc_feature    714..974
                     /vntifkey="21"
                     /label=patial\lumenal\domain\of\ST
     misc_feature    990..1709
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    627..653
                     /vntifkey="21"
                     /label=cytosolic\tail\of\ST
BASE COUNT     1228 a      1369 c      1315 g      1131 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatatg 
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc 
      601 taccggactc agatctcgag gccaccatga ttcacaccaa cctgaagaaa aagttcagct 
      661 gctgcgtact agtcgcagta ctgtttcttc tgtttgcagt gatatgtgtg tggaaggaaa 
      721 agaagaaagg gagttactat gattccttta aattgcaaac caaggaattc caggtgttaa 
      781 agagtctggg gaaattggcc atggggtctg attcccagtc tgtatcctca agcagcaccc 
      841 aggaccccca caggggccgc cagaccctcg gcagtctcag aggcctagcc aaggccaaac 
      901 cagaggcctc cttccaggtg tggaacaagg acagctcttc caaaaacctt atccctaggc 
      961 tgcaaaagat ctgggcaccg gtcgccacca tggtgtccaa gggcgaggag ctgttcaccg 
     1021 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttctccgtgc 
     1081 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atcagcacca 
     1141 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcaga 
     1201 gcttctcccg ctaccccgac cacatgaagc gccacgactt cttcaagagc gccatgcccg 
     1261 aaggctacgt ccaggagcgc accatctcct tcaaggacga cggcacctac aagacccgcg 
     1321 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 
     1381 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac tcccacaacg 
     1441 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 
     1501 acgtggagga cggctccgtg cagctcgccg accactacca gcagaacacc cccatcggcg 
     1561 acggccccgt gctgctgccc gacaaccact acctgtccac ccagtccaag ctgtccaaag 
     1621 accccaacga gaagcgcgat cacatggtcc ttctggaatt cgtgaccgcc gccgggatca 
     1681 ctcacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat 
     1741 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 
     1801 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 
     1861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     1921 tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt 
     1981 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
     2041 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
     2101 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
     2161 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
     2221 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
     2281 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
     2341 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
     2401 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 
     2461 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 
     2521 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 
     2581 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 
     2641 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 
     2701 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 
     2761 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 
     2821 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 
     2881 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 
     2941 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3001 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3061 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3121 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3181 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     3241 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     3301 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     3361 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     3421 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 
     3481 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     3541 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     3601 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     3661 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     3721 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     3781 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     3841 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     3901 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 
     3961 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 
     4021 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 
     4081 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 
     4141 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 
     4201 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 
     4261 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 
     4321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 
     4381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 
     4441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 
     4501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 
     4561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 
     4621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 
     4681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 
     4741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 
     4801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 
     4861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 
     4921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 
     4981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 
     5041 cat 
//
