LOCUS       pAcGFP1N1(w/o\Nd        5046 bp    DNA     linear        1-DEC-2015
DEFINITION  pAcGFP1-N1.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|752179674|
COMMENT     VNTDBDATE|752183982|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pAcGFP1N1(w/o NdeI)-ST6Gal1(Residue1-113)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Created: 12/01/15 06:47PM" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/other+sequences;+artificial+sequences;+vectors.\">other sequences; artificial sequences; vectors.(other sequences; artificial sequences; vectors.)</a>" 
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FEATURES             Location/Qualifiers
     promoter        10..564
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    65..354
                     /vntifkey="21"
                     /label=CAG_enhancer
     misc_feature    521..541
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     promoter        522..591
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    1925..1944
                     /vntifkey="21"
                     /label=EBV_rev_primer
     rep_origin      complement(2094..2400)
                     /vntifkey="33"
                     /label=f1_origin
     promoter        2479..2507
                     /vntifkey="30"
                     /label=AmpR_promoter
     misc_feature    complement(2573..2593)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     misc_feature    complement(2579..2794)
                     /vntifkey="21"
                     /label=SV40_enhancer
     promoter        2591..2859
                     /vntifkey="30"
                     /label=SV40_promoter
     rep_origin      2758..2835
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2820..2839
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     CDS             2942..3736
                     /vntifkey="4"
                     /label=NeoR/KanR
     gene            2945..3733
                     /gene="NeoR/KanR"
                     /vntifkey="60"
     CDS             complement(3251..3787)
                     /vntifkey="4"
                     /label=ORF\frame\3
     terminator      3911..4180
                     /vntifkey="43"
                     /label=TK_PA_terminator
     rep_origin      4328..4947
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_marker     233..240
                     /vntifkey="22"
                     /label=NdeI\abolished\site
     misc_feature    627..977
                     /vntifkey="21"
                     /label=ST6Gal1(Residue\1-113)
     misc_feature    654..716
                     /vntifkey="21"
                     /label=TMD21
     misc_feature    717..977
                     /vntifkey="21"
                     /label=patial\lumenal\domain\of\ST
     misc_feature    993..1712
                     /vntifkey="21"
                     /label=moxGFP
     misc_feature    627..653
                     /vntifkey="21"
                     /label=cytosolic\tail\of\ST
BASE COUNT     1228 a      1370 c      1316 g      1132 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatatg 
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt 
      541 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc 
      601 taccggactc agatctcgag gccaccatga ttcacaccaa cctgaagaaa aagttcagct 
      661 gctgcgtact agtcgcagta ctggtctttc ttctgtttgc agtgatatgt gtgtggaagg 
      721 aaaagaagaa agggagttac tatgattcct ttaaattgca aaccaaggaa ttccaggtgt 
      781 taaagagtct ggggaaattg gccatggggt ctgattccca gtctgtatcc tcaagcagca 
      841 cccaggaccc ccacaggggc cgccagaccc tcggcagtct cagaggccta gccaaggcca 
      901 aaccagaggc ctccttccag gtgtggaaca aggacagctc ttccaaaaac cttatcccta 
      961 ggctgcaaaa gatctgggca ccggtcgcca ccatggtgtc caagggcgag gagctgttca 
     1021 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttctccg 
     1081 tgcggggcga gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatcagca 
     1141 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc 
     1201 agagcttctc ccgctacccc gaccacatga agcgccacga cttcttcaag agcgccatgc 
     1261 ccgaaggcta cgtccaggag cgcaccatct ccttcaagga cgacggcacc tacaagaccc 
     1321 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg 
     1381 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttc aactcccaca 
     1441 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc 
     1501 acaacgtgga ggacggctcc gtgcagctcg ccgaccacta ccagcagaac acccccatcg 
     1561 gcgacggccc cgtgctgctg cccgacaacc actacctgtc cacccagtcc aagctgtcca 
     1621 aagaccccaa cgagaagcgc gatcacatgg tccttctgga attcgtgacc gccgccggga 
     1681 tcactcacgg catggacgag ctgtacaagt aagcggccgc gactctagat cataatcagc 
     1741 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 
     1801 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 
     1861 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     1921 agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat 
     1981 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 
     2041 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 
     2101 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 
     2161 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 
     2221 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 
     2281 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 
     2341 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 
     2401 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     2461 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     2521 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 
     2581 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 
     2641 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 
     2701 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 
     2761 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 
     2821 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 
     2881 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 
     2941 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     3001 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     3061 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     3121 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     3181 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     3241 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     3301 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     3361 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     3421 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 
     3481 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     3541 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     3601 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     3661 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     3721 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     3781 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     3841 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     3901 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 
     3961 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 
     4021 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 
     4081 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 
     4141 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 
     4201 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     4261 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     4321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     4381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     4441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 
     4501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     4561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     4621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     4681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     4741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     4801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     4861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     4921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 
     4981 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     5041 atgcat 
//
