LOCUS       clean_AAV1-hSynI-V5-Tur 5585 bp ds-DNA     circular     18-JAN-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     misc_feature    2840..2847
                     /label="NotI (2)"
     misc_feature    1557..1601
                     /label="Flexible linker"
     CDS             789..1550
                     /label="TurboID-N"
     misc_feature    1884..1979
                     /label="GPI anchor"
     misc_feature    1716..1760
                     /label="Flexible linker"
     misc_feature    643..643
                     /label="SalI"
     repeat_region   1..141
                     /label="AAV2 ITR"
     misc_feature    1818..1862
                     /label="Flexible linker"
     rep_origin      4936..5524
                     /label="ori"
     misc_feature    1767..1811
                     /label="Flexible linker"
     misc_feature    741..782
                     /label="V5 tag_V5 tag"
     misc_feature    2001..2589
                     /label="WPRE"
     repeat_region   2848..2988
                     /label="AAV2 ITR"
     misc_feature    654..659
                     /label="Kozak"
     polyA_signal    2615..2839
                     /label="bGH poly(A) signal"
     promoter        178..625
                     /label="hSyn promoter"
     promoter        3800..3904
                     /label="AmpR promoter"
     misc_feature    660..731
                     /label="Mouse Preproacrosin"
     rep_origin      3063..3518
                     /label="f1 ori"
     CDS             3905..4765
                     /label="AmpR"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgtg tctagactgc agagggccct gcgtatgagt
      181 gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc
      241 cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag
      301 ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac
      361 agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct
      421 gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc
      481 cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga
      541 ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg
      601 agtcgtgtcg tgcctgagag cgcagtcgag aaaccggcta gagtcgaaag cttgccacca
      661 tggtagagat gctgccaact gtcgctgtgc tggtcttggc agtgtccgtg gttgccaagg
      721 ataacaccac gcgtttaaac ggcaagccca tccccaaccc cctgctgggc ctggacagca
      781 ccgctagcaa agacaatact gtgcctctga agctgatcgc tctcctggct aatggcgagt
      841 tccatagtgg cgaacagctg ggagaaaccc tgggcatgtc cagggccgct atcaacaagc
      901 acattcagac tctgcgcgac tggggcgtgg acgtgttcac cgtgcccgga aagggctact
      961 ctctgcccga gcctatcccg ctgctgaacg ctaaacagat tctgggacag ctggacggcg
     1021 ggagcgtggc agtcctgcct gtggtcgact ccaccaatca gtacctgctg gatcgaatcg
     1081 gcgagctgaa gagtggggat gcttgcattg cagaatatca gcaggcaggg agaggaagca
     1141 gagggaggaa atggttctct ccttttggag ctaacctgta cctgagtatg ttttggcgcc
     1201 tgaagcgggg accagcagca atcggcctgg gcccggtcat cggaattgtc atggcagaag
     1261 cgctgcgaaa gctgggagca gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc
     1321 aggatagaaa gctggcaggc atcctggtgg agctggccgg aataacaggc gatgctgcac
     1381 agatcgtcat tggcgccggg attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca
     1441 atcagggctg gatcacactg caggaagcag ggattaacct ggacaggaat actctggccg
     1501 ctacgctgat ccgagagctg cgggcagccc tggaactgtt cgagcaggaa actagtggtg
     1561 gaggcggttc aggcggaggt ggctctggcg gtggcggatc ggctagcgcc accatggccc
     1621 agctcgaaaa ggagctgcaa gccctggaga aggagaacgc ccagctcgaa tgggagctcc
     1681 aggccctgga gaaggagctg gcccagaagg ctagcggtgg aggcggttca ggcggaggtg
     1741 gctctggcgg tggcggatcg tctagcggtg gaggcggttc aggcggaggt ggctctggcg
     1801 gtggcggatc gtctagcggt ggaggcggtt caggcggagg tggctctggc ggtggcggat
     1861 cgtctagcta ccaactcgag aatggcggca taagcctgct ggttcagaac acatcctgga
     1921 tgctgctgct gctgctttcc ctctccctcc tccaagccct ggacttcatt tctctgtagg
     1981 gatccaattc gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2041 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2101 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2161 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2221 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2281 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2341 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     2401 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     2461 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     2521 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     2581 cctccccgca tcgataccgc tcgagagatc tcgactgtgc cttctagttg ccagccatct
     2641 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2701 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2761 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2821 gatgcggtgg gctctatggg cggccgcagg aacccctagt gatggagttg gccactccct
     2881 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     2941 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     3001 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     3061 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3121 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3181 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3241 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     3301 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3361 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     3421 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3481 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     3541 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     3601 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3661 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     3721 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3901 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3961 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4021 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4081 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4141 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4201 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4261 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4321 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4381 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4441 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4501 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4561 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4621 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4681 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4741 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4801 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4861 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4921 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4981 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5041 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5101 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5161 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5221 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5281 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5341 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5401 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5461 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5521 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5581 catgt
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