LOCUS       Exported                4736 bp ds-DNA     circular SYN 17-NOV-2020
DEFINITION  [pBR322].
ACCESSION   urn.local...w-cnu6zrt
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 17, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    3..20
                     /label=Misc_Feature_7
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    3..19
                     /label=araO2 protein
                     /label=araO2 protein; araO2 protein bind
                     /note="araO2"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     98..118
                     /label=DB256
                     /note="/Transferred_From=pDB027a"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     promoter        125..153
                     /label=Promoter_P_2
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    140..156
                     /label=araO1 protein bind
                     /note="araO1"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    161..182
                     /label=Misc_Feature_8
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    161..177
                     /label=araO1 protein bind
                     /note="araO1"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     protein_bind    182..203
                     /label=CAP protein bind
                     /note="CAP"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     misc_feature    193..217
                     /label=380
                     /note="/created_by=User"
     misc_feature    204..217
                     /label=Misc_Feature_9
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    213..251
                     /label=Misc_Feature_10
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     protein_bind    214..230
                     /label=araI1 protein bind
                     /note="araI1"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     protein_bind    235..251
                     /label=araI2 protein bind
                     /note="araI2"
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     promoter        250..277
                     /label=Promoter_P_3
                     /note="/Transferred_From=pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
     CDS             323..1060
                     /label=sfGFP
                     /note="/Transferred_From=sfGFP–chenguang"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(383..403)
                     /label=DB541
     misc_feature    437..439
                     /label=N39
                     /note="/created_by=User"
     misc_feature    725..727
                     /label=N135
                     /note="/created_by=User"
     misc_feature    773..775
                     /label=Y151
                     /note="/created_by=User"
     primer_bind     complement(1024..1044)
                     /label=DB540
     terminator      1105..1529
                     /label=rrnB1 transcriptional terminator
                     /note="rrnB1 transcriptional terminator"
                     /note="/Transferred_From=pAB081j"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(1114..1138)
                     /label=381
                     /note="/created_by=User"
     promoter        complement(1538..1622)
                     /label=aadA promoter
                     /note="aadA promoter"
                     /note="/Transferred_From=pDB009, pDB009a, pDB009b, 
                     pDB009c,pDB009d, pDB009e, pDB009f, pDB009f1, pDB009f2, 
                     pDB009f3, pDB009g, pDB009h, pDB009i, pDB009j, pDB009k, 
                     pDB009k1, pDB009k2, pDB009k3, pDB010a (multiple clones need
                     sequencing), pDB012a (multiple clones need sequencing), 
                     pDB013f4 (not made), pDB013f8 (not made), pDB014f4 (not 
                     made), pDB014f8 (not made), pDB015f2 (***Contains bad 
                     mutations T7RNAP), pDB016, pDB016r (not made yet), pDB018a,
                     pDB018b, pDB018c, pDB018d, pDB023f, pDB023f1, pDB023f2, 
                     pDB023f3, pDB023k, pDB023k1, pDB023k2, pDB023k3, pDB024f, 
                     pDB024f2, pDB028, pDB028ns1, pDB028ns2, pDB028ns3, 
                     pDB028ns4, pDB028ns5, pDB030f, pDB033ns1, pDB035, pDB038, 
                     pDB038a, pDB038b, pDB039, pDB040, pDB042 (not made), 
                     pDB045ns1, pDB045ns1.2 (not made), pDB046c, pDB046c(–), 
                     pDB050a (wait to clone), pDB050b (wait to clone), pDB050c 
                     (wait to clone), pDB050d (wait to clone), pDB052 (wait to 
                     clone), pDB052a, pDB052b, pDB052c, pDB052d, 
                     pDB055a1-from38, pDB055b1-from38a, pDB056, pDB060, 
                     pDB060IF, pDB060IF_IPYE, pDB060Mb, pDB060Mb_tetra, 
                     pDB060Mm, pDB060Mm_tetra, pDB060Y349F, pDB060tetra, 
                     pDB060tetra/349F, pDB061a, pDB061b, pDB061c, pDB061d, 
                     pDB063.pIF, pDB063.pNF"
                     /note="/Transferred_Similarity=100.00%"
     promoter        1553..1581
                     /label=Promoter
                     /note="/Transferred_From=pCMV-PylRST_iRFP.dib 
                     (modified),pCMV-PylRST_mCherry.dib (modified), 
                     pCMV-mCherry.dbt (modified)"
                     /note="/Transferred_Similarity=100.00%"
     CDS             1623..2483
                     /label=bla (AmpR)
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(1693..1710)
                     /label=Seq.Forward 8
                     /note="/Transferred_From=pDB027a"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(1693..1710)
                     /label=pDB1TetSeq.Forward8
                     /note="/Transferred_From=pDB11"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(1896..1916)
                     /label=DB448
     primer_bind     complement(2100..2118)
                     /label=RAJ-Seq.6
                     /note="/Transferred_From=pDB11"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(2388..2407)
                     /label=Seq.Forward 7
                     /note="/Transferred_From=pDB027a"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(2388..2407)
                     /label=pDB1TetSeq.Forward7
                     /note="/Transferred_From=pDB11"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     2388..2407
                     /label=Seq.Reverse 7
                     /note="/Transferred_From=pDB005CHx"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     rep_origin      2638..3257
                     /label=pBR322 origin
                     /note="/Transferred_From=piRFP670-N1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(2725..2746)
                     /label=DB447
     primer_bind     complement(2923..2942)
                     /label=tRNA_Seq.Forward3
                     /note="/Transferred_From=HHR-plasmid"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(2924..2942)
                     /label=Seq.Forward13
                     /note="/Transferred_From=pDB038b"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(3346..3366)
                     /label=DB446
     primer_bind     complement(3476..3496)
                     /label=Seq.Forward12
                     /note="/Transferred_From=pDB038b"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     CDS             complement(3832..4710)
                     /label=araC
                     /note="/Transferred_From=pAB081j, pDB027, pDB027(–), 
                     pDB027a,pDB027a(–), pDB027b, pDB027b(–), pDB027c, 
                     pDB027c(–), pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy,
                     pDB032c(–), pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, 
                     pDB046c, pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c, pJC166d - T7 RNAP redon"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
                     /note="/dnas_title=araC"
                     /note="/vntifkey=4"
     misc_feature    3832..3872
                     /label=misc
                     /note="/Transferred_From=pDB027, pDB027(–), pDB027a, 
                     pDB027a(–),pDB027b, pDB027b(–), pDB027c, pDB027c(–), 
                     pDB027c.IodoPhe, pDB032, pDB032c, pDB032c Copy, pDB032c(–),
                     pDB032d, pDB032e, pDB032f, pDB032g, pDB032h, pDB046c, 
                     pDB046c(–), pDB059, pDB059GFP, pDB059GFP.2TAGb, 
                     pDB059GFP.3TAGb, pDB059GFP.N39*, pDB059GFP.S2*, 
                     pDB059GFP.Y151*, pDB059GFP135*, pDB059GFP2TAG, 
                     pDB059GFP3TAG, pDB059c"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(3915..3935)
                     /label=DB543
     primer_bind     4296..4318
                     /label=DB255
                     /note="/Transferred_From=pDB027a"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=User"
     primer_bind     complement(4455..4475)
                     /label=DB542
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccta ggatgagcaa gggcgaagaa ctgtttacgg gcgtggtgcc
      361 gattctggtg gaactggatg gtgatgtcaa tggtcacaaa ttcagcgtgc gcggcgaagg
      421 tgaaggcgat gcaaccaatg gtaaactgac gctgaagttt atttgcacca cgggtaaact
      481 gccggttccg tggccgaccc tggtcaccac gctgacgtat ggtgttcagt gtttcagtcg
      541 ttacccggat cacatgaaac gccacgactt tttcaagtcc gcgatgccgg aaggttatgt
      601 ccaagaacgt accatctcat ttaaagatga cggcacctac aaaacgcgcg ccgaagtgaa
      661 attcgaaggt gatacgctgg ttaaccgtat tgaactgaaa ggcatcgatt ttaaggaaga
      721 cggtaatatt ctgggccata aactggaata taacttcaat tcgcacaacg tgtacatcac
      781 cgcagataag cagaagaacg gtatcaaggc taacttcaag atccgccata atgtggaaga
      841 tggcagcgtt caactggccg accactatca gcaaaacacc ccgattggtg atggcccggt
      901 cctgctgccg gacaatcatt acctgagcac gcagtctgtg ctgagtaaag atccgaacga
      961 aaagcgtgac cacatggtcc tgctggaatt cgtgaccgcg gccggcatca cgcacggtat
     1021 ggacgaactg tataaaggct cacatcatca tcatcatcat tgactcgaga tctgcagctg
     1081 gtaccatatg ggaattcgaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     1141 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     1201 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     1261 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     1321 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1381 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     1441 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     1501 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
     1561 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1621 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1681 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1741 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1801 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1861 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1921 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1981 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2041 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2101 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2161 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2221 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2281 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2341 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2401 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2461 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2521 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2581 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2761 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3421 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     3481 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     3541 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     3601 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     3661 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc
     3721 gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct
     3781 gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa
     3841 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
     3901 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
     3961 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
     4021 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
     4081 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     4141 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     4201 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     4261 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     4321 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     4381 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     4441 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     4501 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     4561 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     4621 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     4681 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagag
//
