LOCUS       Exported                4415 bp ds-DNA     circular SYN 17-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...oq-aaz2pab
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 17, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(join(1..6,4398..4415))
                     /label=821
     misc_feature    59..919
                     /label=amp marker
     misc_feature    1074..1693
                     /label=pBR322 origin
     terminator      1817..1974
                     /label=rrnB term
     misc_feature    1998..2024
                     /label=822
     misc_feature    2096..2120
                     /label=868
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(2162..2209)
                     /label=825
     misc_feature    2180..2451
                     /label=gBlock for IG
                     /note="/modified_by=User"
     rep_origin      2180..2449
                     /label=Move this upstream100bp+PlldR in to pJKR
     misc_feature    2247..2263
                     /label=lldRO1
     misc_feature    2318..2323
                     /label=-35 (<c)
                     /label=-35 (
                     /note="/modified_by=User"
     misc_feature    2341..2346
                     /label=-10 (B/C)
                     /note="/modified_by=User"
     misc_feature    2352
                     /label=+1
     misc_feature    2373..2389
                     /label=lldRO2
     misc_feature    2422..2477
                     /label=824
     misc_feature    complement(2444..2502)
                     /label=J140
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     rep_origin      2452..2477
                     /label=g10 RBS
     misc_feature    2478..3209
                     /label=gBlock726.sfGFP.15Leu.15TAG
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
     misc_feature    2496..3209
                     /label=CTGTTCACCGGTGTTGTTCCGATCCTGGTTGAACTGGACGGTGACGT
                     /label=CTGTTCACCGGTGTTGTTCCGATCCTGGTTGAACTGGACGGTGACGTTAA 
                     CGGTCACAAATTCTCTGTTCGTGGTGAAGGTGAAGGTGACGCTACCAACGGTAAACTG 
                     ACCCTGAAATTCATCTGCACCACCGGTAAACTGCCGGTTCCGTGGCCGACCCTGGTTA 
                     CCACCCTGACCTACGGTGTTCAGTGCTTCGCTCGTTACCCGGACCACATGAAACAGCA 
                     CGACTTCTTCAAATCTGCTATGCCGGAAGGTTACGTTCAGGAACGTACCATCTCTTTC 
                     AAAGACGACGGTACCTACAAAACCCGTGCTGAAGTTAAATTCGAAGGTGACACCCTGG 
                     TTAACCGTATCGAACTGAAAGGTATCGACTTCAAAGAAGACGGTAACATCCTGGGTCA 
                     CAAACTGGAATACAACTTCAACTCTCACAACGTTTACATCACCGCTGACAAACAGAAA 
                     AACGGTATCAAAGCTAACTTCAAAATCCGTCACAACGTTGAAGACGGTTCTGTTCAGC 
                     TGGCTGACCACTACCAGCAGAACACCCCGATCGGTGACGGTCCGGTTCTGCTGCCGGA 
                     CAACCACTACCTGTCTACCCAGTCTGTTCTGTCTAAAGACCCGAACGAAAAACGTGAC 
                     CACATGGTTCTGCTGGAATTCGTTACCGCTGCTGGTATCACCCACGGTATGGACGAAC 
                     TGTACAAAGATGCATGCCAGTTCTAG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CTGTTCACCGGTGTTGTTCCGATCCTGGTTGAACTG
                     GACGG 
                     TGACGTTAACGGTCACAAATTCTCTGTTCGTGGTGAAGGTGAAGGTGACGCTACCAAC 
                     GGTAAACTGACCCTGAAATTCATCTGCACCACCGGTAAACTGCCGGTTCCGTGGCCGA 
                     CCCTGGTTACCACCCTGACCTACGGTGTTCAGTGCTTCGCTCGTTACCCGGACCACAT 
                     GAAACAGCACGACTTCTTCAAATCTGCTATGCCGGAAGGTTACGTTCAGGAACGTACC 
                     ATCTCTTTCAAAGACGACGGTACCTACAAAACCCGTGCTGAAGTTAAATTCGAAGGTG 
                     ACACCCTGGTTAACCGTATCGAACTGAAAGGTATCGACTTCAAAGAAGACGGTAACAT 
                     CCTGGGTCACAAACTGGAATACAACTTCAACTCTCACAACGTTTACATCACCGCTGAC 
                     AAACAGAAAAACGGTATCAAAGCTAACTTCAAAATCCGTCACAACGTTGAAGACGGTT 
                     CTGTTCAGCTGGCTGACCACTACCAGCAGAACACCCCGATCGGTGACGGTCCGGTTCT 
                     GCTGCCGGACAACCACTACCTGTCTACCCAGTCTGTTCTGTCTAAAGACCCGAACGAA 
                     AAACGTGACCACATGGTTCTGCTGGAATTCGTTACCGCTGCTGGTATCACCCACGGTA 
                     TGGACGAACTGTACAAAGATGCATGCCAGTTCTAG"
     misc_feature    2886..2888
                     /label=Start reverting from here for 10Leu
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
     misc_feature    3078..3209
                     /label=Revert last 5 ambers to WT, for 15Leu.15TAG
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    3078..3209
                     /label=EGFP-F reporter
     misc_feature    3078..3080
                     /label=Start reverting from here for 15Leu
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
     misc_feature    3187..3245
                     /label=J139
                     /label=nonstandard type: Marker
                     /note="/created_by=User"
     misc_feature    3207..3209
                     /label=QC0_Terminal TAA
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    complement(3321..3344)
                     /label=869
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3359..3384
                     /label=820
     rep_origin      3398..3479
                     /label=proA
                     /note="/modified_by=User"
     misc_feature    3398..3426
                     /label=proA -35 to -10
     misc_feature    3398..3403
                     /label=-35 (<d)
                     /label=-35 (
                     /note="/modified_by=User"
     misc_feature    3421..3426
                     /label=-10 (<B)
                     /label=-10 (
                     /note="/modified_by=User"
     misc_feature    complement(3459..3507)
                     /label=813
     misc_feature    3478..3527
                     /label=810
     rep_origin      3480..3499
                     /label=Biosensor RBS
     rep_origin      3486..3491
                     /label=RBS
     misc_feature    3500..4276
                     /label=LldR
     misc_feature    complement(4244..4291)
                     /label=811
     misc_feature    4262..4310
                     /label=812
     terminator      4335..4378
                     /label=T7 term
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     2161 gctagcaagt aaggccgacc aacaacctaa gggcaattct ctgatgagga ttgccctttt
     2221 ctttaccaga catctccccc cacaagaatt ggccctacca attcttcgct tatctgacct
     2281 ctggttcaca atttcccaat taaaactcac atcaatgttg ccaatacata acatttagtt
     2341 aaccattcat tgtcattatc cctacacaac acaattggca gtgccacttt tacacaacgt
     2401 gtgacaagga gatgagcaac agactcatta cacgatgtgc gtggactccc atttaacttt
     2461 aagaaggaga tatacatatg cgtaaaggtg aagaatagtt caccggtgtt gttccgatct
     2521 aggttgaata ggacggtgac gttaacggtc acaaattctc tgttcgtggt gaaggtgaag
     2581 gtgacgctac caacggtaaa tagacctaga aattcatctg caccaccggt aaatagccgg
     2641 ttccgtggcc gacctaggtt accacctaga cctacggtgt tcagtgcttc gctcgttacc
     2701 cggaccacat gaaacagcac gacttcttca aatctgctat gccggaaggt tacgttcagg
     2761 aacgtaccat ctctttcaaa gacgacggta cctacaaaac ccgtgctgaa gttaaattcg
     2821 aaggtgacac ctaggttaac cgtatcgaat agaaaggtat cgacttcaaa gaagacggta
     2881 acatctaggg tcacaaatag gaatacaact tcaactctca caacgtttac atcaccgctg
     2941 acaaacagaa aaacggtatc aaagctaact tcaaaatccg tcacaacgtt gaagacggtt
     3001 ctgttcagta ggctgaccac taccagcaga acaccccgat cggtgacggt ccggtttagt
     3061 agccggacaa ccactacctg tctacccagt ctgttctgtc taaagacccg aacgaaaaac
     3121 gtgaccacat ggttctgctg gaattcgtta ccgctgctgg tatcacccac ggtatggacg
     3181 aactgtacaa agatgcatgc cagttctaac ataaccctaa tgagtgagct aacttacatt
     3241 aattgcgttg cgccttaatt aacggcactc ctcagcaaat ataatgaccc tcttgataac
     3301 ccaagagggc attttttaat gcccatggcg tttaccacag ctaacaccac gtcgtcccta
     3361 tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc ataaggctcg
     3421 taggctatat tcagggagac cacaacggtt tccctctaca aataattttg tttaactttg
     3481 aaataaggag gtaatacaaa tgattgtttt acccagacgc ctgtcagacg aggttgccga
     3541 tcgtgtgcgg gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga
     3601 gcgccaactg gcgatgcaac tcggcgtatc acgtaattca ctgcgcgagg cgctggcaaa
     3661 actggtgagt gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg
     3721 tcatgacaca tggtcggagc aaaacatcgt ccagccgcta aaaacactga tggccgatga
     3781 tccggattac agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg
     3841 gcatgcggca atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc
     3901 aacgctaagt gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat
     3961 tgccgaagcc tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct
     4021 gcaatcctca gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca
     4081 actgaccgaa caacatcagg ctgtcattga cgccattttt gccggtgatg ctgacggggc
     4141 gcgtaaagca atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga
     4201 agatcaggct cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag
     4261 ggagaaaaac gcataataag cgactaaaaa attgaatgta ggaaaccaac atgccagttc
     4321 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     4381 aaacctcagg catttgagaa gcacacggtc acact
//
