LOCUS       Exported                4381 bp ds-DNA     circular SYN 17-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...oq-aaz2pab
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 17, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(join(1..6,4364..4381))
                     /label=821
     misc_feature    59..919
                     /label=amp marker
     misc_feature    1074..1693
                     /label=pBR322 origin
     terminator      1817..1974
                     /label=rrnB term
     misc_feature    1998..2024
                     /label=822
     misc_feature    2096..2120
                     /label=868
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    complement(2162..2209)
                     /label=825
     misc_feature    2180..2417
                     /label=gBlock for IG
                     /note="/modified_by=User"
     rep_origin      2180..2415
                     /label=Move this upstream100bp+PlldR in to pJKR
     misc_feature    complement(2221..2257)
                     /label=J249
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    2238..2315
                     /label=J246
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    2246^2247
                     /label=AATTGGCCCTACCAATT
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=AATTGGCCCTACCAATT"
     misc_feature    complement(2280..2369)
                     /label=J247
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    2301..2306
                     /label=-35 (<c)
                     /label=-35 (
                     /note="/modified_by=User"
     misc_feature    2324..2329
                     /label=-10 (B/C)
                     /note="/modified_by=User"
     misc_feature    2335
                     /label=+1
     misc_feature    2345..2386
                     /label=J248
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    2355^2356
                     /label=AATTGGCAGTGCCACTT
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=AATTGGCAGTGCCACTT"
     misc_feature    2388..2453
                     /label=824
     rep_origin      2418..2443
                     /label=g10 RBS
     misc_feature    2444..3175
                     /label=EGFP-F reporter
     misc_feature    complement(2573..2600)
                     /label=823
     misc_feature    3173..3175
                     /label=QC0_Terminal TAA
                     /label=nonstandard type: motif
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    3175
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     misc_feature    complement(3287..3310)
                     /label=869
                     /label=nonstandard type: motif
                     /note="/created_by=User"
     misc_feature    3325..3350
                     /label=820
     rep_origin      3364..3445
                     /label=proA
                     /note="/modified_by=User"
     misc_feature    3364..3392
                     /label=proA -35 to -10
     misc_feature    3364..3369
                     /label=-35
     misc_feature    3387..3392
                     /label=-10
     misc_feature    complement(3425..3473)
                     /label=813
     misc_feature    3444..3493
                     /label=810
     rep_origin      3446..3465
                     /label=Biosensor RBS
     rep_origin      3452..3457
                     /label=RBS
     misc_feature    3466..4242
                     /label=LldR
     misc_feature    complement(4210..4257)
                     /label=811
     misc_feature    4228..4276
                     /label=812
     terminator      4301..4344
                     /label=T7 term
ORIGIN
        1 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
       61 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      121 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      181 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      241 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      301 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      361 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      421 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      481 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      541 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
      601 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
      661 ccggcaacaa ttgatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
      721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
      781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
      841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
      901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
      961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1021 caaaatccct taacgtgggt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1081 aggatcatct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcaggctgt
     1741 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     1801 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     1861 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     1921 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1981 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     2041 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     2101 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     2161 gctagcaagt aaggccgacc aacaacctaa gggcaattct ctgatgagga ttgccctttt
     2221 ctttaccaga catctccccc cacaagcttc gcttatctga cctctggttc acaatttccc
     2281 aattaaaact cacatcaatg ttgccaatac ataacattta gttaaccatt cattgtcatt
     2341 atccctacac aacacttaca caacgtgtga caaggagatg agcaacagac tcattacacg
     2401 atgtgcgtgg actcccattt aactttaaga aggagatata catatgcgta aaggtgaaga
     2461 actgttcacc ggtgttgttc cgatcctggt tgaactggac ggtgacgtta acggtcacaa
     2521 attctctgtt cgtggtgaag gtgaaggtga cgctaccaac ggtaaactga ccctgaaatt
     2581 catctgcacc accggtaaac tgccggttcc gtggccgacc ctggttacca ccctgaccta
     2641 cggtgttcag tgcttcgctc gttacccgga ccacatgaaa cagcacgact tcttcaaatc
     2701 tgctatgccg gaaggttacg ttcaggaacg taccatctct ttcaaagacg acggtaccta
     2761 caaaacccgt gctgaagtta aattcgaagg tgacaccctg gttaaccgta tcgaactgaa
     2821 aggtatcgac ttcaaagaag acggtaacat cctgggtcac aaactggaat acaacttcaa
     2881 ctctcacaac gtttacatca ccgctgacaa acagaaaaac ggtatcaaag ctaacttcaa
     2941 aatccgtcac aacgttgaag acggttctgt tcagctggct gaccactacc agcagaacac
     3001 cccgatcggt gacggtccgg ttctgctgcc ggacaaccac tacctgtcta cccagtctgt
     3061 tctgtctaaa gacccgaacg aaaaacgtga ccacatggtt ctgctggaat tcgttaccgc
     3121 tgctggtatc acccacggta tggacgaact gtacaaagat gcatgccagt tctaacataa
     3181 ccctaatgag tgagctaact tacattaatt gcgttgcgcc ttaattaacg gcactcctca
     3241 gcaaatataa tgaccctctt gataacccaa gagggcattt tttaatgccc atggcgttta
     3301 ccacagctaa caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat
     3361 aactttacgg gcatgcataa ggctcgtagg ctatattcag ggagaccaca acggtttccc
     3421 tctacaaata attttgttta actttgaaat aaggaggtaa tacaaatgat tgttttaccc
     3481 agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg
     3541 gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg cgtatcacgt
     3601 aattcactgc gcgaggcgct ggcaaaactg gtgagtgaag gcgtgctgct cagtcgacgc
     3661 ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa catcgtccag
     3721 ccgctaaaaa cactgatggc cgatgatccg gattacagtt tcgatattct ggaagcccgc
     3781 tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa
     3841 gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat cgcctcacaa
     3901 gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa
     3961 accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg tcagcggatg
     4021 tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt cattgacgcc
     4081 atttttgccg gtgatgctga cggggcgcgt aaagcaatga tggcgcacct tagttttgtt
     4141 cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat tacccgcctg
     4201 cccggtgagc ataatgagca ttcgagggag aaaaacgcat aataagcgac taaaaaattg
     4261 aatgtaggaa accaacatgc cagttcgagc aataactagc ataacccctt ggggcctcta
     4321 aacgggtctt gaggggtttt ttgctgaaac ctcaggcatt tgagaagcac acggtcacac
     4381 t
//
