LOCUS       Exported                4916 bp ds-DNA     circular SYN 17-NOV-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 17, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        143..172
                     /label=lac_promoter
                     /note="color: #ff9ccd"
     misc_feature    239..256
                     /label=Sp6_primer
                     /note="color: #faac61"
     promoter        complement(434..678)
                     /label=hU6_promoter
                     /note="color: #faac61; direction: LEFT"
     promoter        complement(803..821)
                     /label=T7_promoter
                     /note="color: #faac61; direction: LEFT"
     misc_feature    825..968
                     /label=lacZ_a
                     /note="color: #faac61"
     misc_feature    991..1284
                     /label=ccdB
                     /note="color: #b7e6d7"
     promoter        1495..1544
                     /label=NEOKAN_promoter
                     /note="color: #84b0dc"
     gene            1636..2424
                     /label=NeoR/KanR
                     /note="color: #ffef86; direction: RIGHT"
     enhancer        2584..2887
                     /label=CMV enhancer
                     /note="color: #d59687"
     promoter        2888..3091
                     /label=CMV promoter
                     /note="color: #84b0dc"
     misc_feature    3126..3827
                     /label=tagBFP
                     /note="color: #84b0dc"
     polyA_signal    3949..4070
                     /label=SV40 poly(A) signal
                     /note="color: #d6b295"
     misc_feature    4131..4750
                     /label=pBR322_origin
                     /note="color: #85dae9"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag
      361 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      421 cttattttaa cttaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      481 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      541 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
      601 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      661 ctcttcctgc ccgaccttgg taccggatcc agtcgactga attggttcct ttaaagcctg
      721 cttttttgta caaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat
      781 gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
      961 ttgcgcagcc tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat
     1021 cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc
     1081 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     1141 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     1201 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     1261 aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc
     1321 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     1381 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     1441 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     1501 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     1561 ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     1621 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     1681 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     1741 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     1801 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     1861 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     1921 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     1981 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     2041 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     2101 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     2161 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     2221 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     2281 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     2341 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     2401 tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat
     2461 tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa
     2521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2581 tgacgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2641 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2701 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2761 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2821 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2881 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2941 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3001 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3061 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     3121 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     3181 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3241 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     3301 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     3361 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     3421 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     3481 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     3541 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     3601 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     3661 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     3721 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     3781 ccagatactg cgacctccct agcaaactgg ggcacaagct taattaggcg gccgcgactc
     3841 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3901 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3961 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4021 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgaccaa
     4081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4741 cagcaacgcg gcctttttac ggttcctggg cttttgctgg ccttttgctc acatgttctt
     4801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
