LOCUS       Exported                4976 bp ds-DNA     circular SYN 17-NOV-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 17, 2020 from SnapGene Viewer 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        143..172
                     /label=lac_promoter
                     /note="color: #ff9ccd"
     misc_feature    239..256
                     /label=Sp6_primer
                     /note="color: #faac61"
     misc_feature    414..514
                     /label=gRNA
                     /note="color: #ff9ccd"
     misc_feature    414..433
                     /label=Left Homology
                     /note="color: #85dae9"
     misc_feature    454..473
                     /label=gRNA
                     /note="color: #b7e6d7"
     misc_feature    494..514
                     /label=Right homology
                     /note="color: #ffef86"
     promoter        complement(515..738)
                     /label=hU6_promoter
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(533..552)
                     /label=LKO1_5_primer
                     /note="color: #c6c9d1; direction: LEFT"
     promoter        complement(863..881)
                     /label=T7_promoter
                     /note="color: #faac61; direction: LEFT"
     misc_feature    885..1028
                     /label=lacZ_a
                     /note="color: #faac61"
     promoter        complement(888..904)
                     /label=M13_forward20_primer
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    1051..1344
                     /label=ccdB
                     /note="color: #b7e6d7"
     promoter        1555..1604
                     /label=NEOKAN_promoter
                     /note="color: #84b0dc"
     gene            1696..2484
                     /label=NeoR/KanR
                     /note="color: #ffef86; direction: RIGHT"
     enhancer        2644..2947
                     /label=CMV enhancer
                     /note="color: #d59687"
     promoter        2948..3151
                     /label=CMV promoter
                     /note="color: #84b0dc"
     misc_feature    3186..3887
                     /label=tagBFP
                     /note="color: #84b0dc"
     polyA_signal    4009..4130
                     /label=SV40 poly(A) signal
                     /note="color: #d6b295"
     primer_bind     complement(4046..4065)
                     /label=SV40pA-R
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    4191..4810
                     /label=pBR322_origin
                     /note="color: #85dae9"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag
      361 ctgggtctag aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
      421 cttattttaa cttgctattt ctagctctaa aacggttatg ccaggtagta gtccggtgtt
      481 tcgtcctttc cacaagatat ataaagccaa gaaatcgaaa tactttcaag ttacggtaag
      541 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      601 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
      661 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      721 ctcttcctgc ccgaccttgg taccggatcc agtcgactga attggttcct ttaaagcctg
      781 cttttttgta caaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat
      841 gcatctagag ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
      901 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      961 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1021 ttgcgcagcc tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat
     1081 cgtctgtttg tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc
     1141 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     1201 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     1261 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     1321 aacctgatgt tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc
     1381 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     1441 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     1501 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     1561 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     1621 ttctcgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     1681 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     1741 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     1801 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     1861 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     1921 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     1981 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     2041 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     2101 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     2161 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     2221 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     2281 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     2341 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     2401 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     2461 tatcgccttc ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat
     2521 tttctcctta cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa
     2581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2641 tgacgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2701 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2761 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2821 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2881 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2941 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     3001 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     3061 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     3121 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     3181 ccaccatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     3241 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     3301 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     3361 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     3421 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     3481 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     3541 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     3601 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     3661 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     3721 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     3781 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     3841 ccagatactg cgacctccct agcaaactgg ggcacaagct taattaggcg gccgcgactc
     3901 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3961 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4021 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4081 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgaccaa
     4141 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     4201 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     4261 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     4321 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     4381 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4441 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4501 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4561 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4621 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4681 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4741 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4801 cagcaacgcg gcctttttac ggttcctggg cttttgctgg ccttttgctc acatgttctt
     4861 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4921 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
