LOCUS       Exported                3180 bp ds-DNA     circular SYN 08-JUN-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Karen Polizzi's Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2021 from SnapGene 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    147..209
                     /label=SP44
     primer_bind     complement(207..238)
                     /label=NTG_GFP or mScarlet_R
     misc_feature    210..236
                     /label=PET-RBS
                     /note="pET3a RBS"
     misc_feature    243..937
                     /label=mScarlet-I
     primer_bind     243..260
                     /label=NTG_mScarlet_F
     CDS             944..967
                     /codon_start=1
                     /product="FLAG(R) epitope tag"
                     /label=Flag tag
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             968..985
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=FlAsH tag
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     misc_feature    989..1093
                     /label=dBroccoli
     repeat_region   1100..1103
                     /label=GG scar
     terminator      1112..1183
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1199..1226
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1430..2018)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2189..3049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3050..3154)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
       61 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      121 cgcttctaga acgtctcaat ctctattgtt cacattcgaa ccgtctctgc tttgacaaca
      181 tgctgtgcgg tgttgtaaag tctggtgtag tactttaact ttaagaagga gatatacaca
      241 tatggtgtcc aagggcgagg ccgtcatcaa ggagttcatg cgcttcaagg tccacatgga
      301 gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccgtacga
      361 gggcacccag accgccaagc tgaaggtcac caagggcggc ccgctgccgt tctcctggga
      421 catcctgtcc ccgcagttca tgtacggctc ccgcgccttc atcaagcacc cggccgacat
      481 cccggactac tacaagcagt ccttcccgga gggcttcaag tgggagcgcg tcatgaactt
      541 cgaggacggc ggcgccgtca ccgtcaccca ggacacctcc ctggaggacg gcaccctgat
      601 ctacaaggtc aagctgcgcg gcaccaactt cccgccggac ggcccggtca tgcagaagaa
      661 gaccatgggc tgggaggcct ccaccgagcg cctgtacccg gaggacggcg tcctgaaggg
      721 cgacatcaag atggccctgc gcctgaagga cggcggccgc tacctggccg acttcaagac
      781 cacctacaag gccaagaagc cggtccagat gccgggcgcc tacaacgtcg accgcaagct
      841 ggacatcacc tcccacaacg aggactacac cgtcgtcgag cagtacgagc gctccgaggg
      901 ccgccactcc accggcggca tggacgagct gtacaaggga tccgactaca aggacgacga
      961 cgacaagtgc tgcccgggct gctgctaatt gccatgtgta tgtgggagac ggtcgggtcc
     1021 agatattcgt atctgtcgag tagagtgtgg gctcccacat actctgatga tccttcggga
     1081 tcattcatgg caaagatcct cgaccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1141 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1201 ctcaccttcg ggtgggcctt tctgcgttta tatgtttgcc agagacgctg caggcttcct
     1261 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1321 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1381 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1441 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1501 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1561 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1621 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1681 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1741 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1801 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1861 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1921 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1981 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2041 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2101 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2161 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2221 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2281 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gagccacgct
     2341 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2401 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2461 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2521 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2581 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2641 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2701 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2761 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2821 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2881 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2941 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3001 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
