LOCUS       Exported                7962 bp ds-DNA     circular SYN 18-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 18, 2020 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7962
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1422..1877
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2159..2263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2264..3124
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3295..3883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_recomb     4446..5273
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             4446..4570
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(4608..5213)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        5277..5368
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     5301..5320
                     /label=Bac1
     CDS             5380..6036
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MASPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNK
                     KFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIR
                     YGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVV
                     LYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             6052..6075
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    6091..6215
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        6240..6270
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6324..6983
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             7325..7630
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7671..7795)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7803..7832
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_recomb     join(7880..7962,1..1275)
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(join(7896..7962,1..1275))
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
ORIGIN
        1 aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta
       61 tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca
      121 aacaagggtt gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc
      181 acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg
      241 ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca
      301 ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta
      361 acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta
      421 gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct
      481 aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt
      541 ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc
      601 gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga
      661 gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc
      721 ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca
      781 gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga
      841 cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt
      901 gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt
      961 ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc
     1021 gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc
     1081 gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat
     1141 tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta
     1201 tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat
     1261 tgtctcaagc tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc
     1321 attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc
     1381 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     1441 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1501 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1561 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1621 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1681 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1741 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     1801 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1861 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     1921 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1981 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     2041 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     2101 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     2161 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2221 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2281 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     2341 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     2401 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     2461 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     2521 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     2581 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     2641 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     2701 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     2761 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     2821 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     2881 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     2941 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3001 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3061 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3121 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3181 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3241 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3301 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3361 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3421 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     3481 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3541 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3601 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3661 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3721 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3841 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3901 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3961 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4021 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4081 accgcctctc cccgcgcgtt ggccgattca ttaatgcaga acggctccgc ccactattaa
     4141 tgaaattaaa aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg
     4201 cgtagaagga aagaaaaatg tcgtcgacat gctgaacaac aagattaata tgcctccgtg
     4261 tataaaaaaa atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag
     4321 gaagaggttt atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa
     4381 ccgatgttta atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga
     4441 agtcatgcat cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat
     4501 gaaaactgtc gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg
     4561 taattattga ataataaaac aattataaat gctaaatttg ttttttatta acgatacaaa
     4621 ccaaacgcaa caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg
     4681 ctgaggtaat atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt
     4741 ttattttcac ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca
     4801 tttttctcct cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat
     4861 agagtaaatt ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg
     4921 ctgcgcatag tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt
     4981 gttgctttaa ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat
     5041 atgttgccgg catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc
     5101 ggattaacat aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc
     5161 ttttctatac tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg
     5221 agacgcacaa actaatatca caaactggaa atgtctatca atatatagtt gctgatatca
     5281 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     5341 cagttttgta ataaaaaaac ctataaatac ggatctacca tggcctcccc tatactaggt
     5401 tattggaaaa ttaagggcct tgtgcaaccc actcgacttc ttttggaata tcttgaagaa
     5461 aaatatgaag agcatttgta tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt
     5521 gaattgggtt tggagtttcc caatcttcct tattatattg atggtgatgt taaattaaca
     5581 cagtctatgg ccatcatacg ttatatagct gacaagcaca acatgttggg tggttgtcca
     5641 aaagagcgtg cagagatttc aatgcttgaa ggagcggttt tggatattag atacggtgtt
     5701 tcgagaattg catatagtaa agactttgaa actctcaaag ttgattttct tagcaagcta
     5761 cctgaaatgc tgaaaatgtt cgaagatcgt ttatgtcata aaacatattt aaatggtgat
     5821 catgtaaccc atcctgactt catgttgtat gacgctcttg atgttgtttt atacatggac
     5881 ccaatgtgcc tggatgcgtt cccaaaatta gtttgtttta aaaaacgtat tgaagctatc
     5941 ccacaaattg ataagtactt gaaatccagc aagtatatag catggccttt gcagggctgg
     6001 caagccacgt ttggtggtgg cgaccatcct ccaaaatcgg attccgcggg tctggaagtt
     6061 ctgttccagg ggccccatat ggtttatcaa acaagtttgt acaaaaaagc tgaacgagaa
     6121 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     6181 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     6241 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     6301 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     6361 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     6421 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     6481 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     6541 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     6601 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     6661 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     6721 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     6781 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     6841 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     6901 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     6961 atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc agataacagt
     7021 atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg tatacccgaa
     7081 gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg acagcgacag
     7141 ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag cacaaccatg
     7201 cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg
     7261 gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa caggggctgg
     7321 tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc tgtttgtgga
     7381 tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc tggccagtgc
     7441 acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata tcggggatga
     7501 aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta tcggggaaga
     7561 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
     7621 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
     7681 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
     7741 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtaggat
     7801 ccgaacaaaa actcatctca gaagaggatc tgtaaaacgg atccgaattc ccgggcggcc
     7861 gcttaattaa ttgatccggg ttattagtac atttattaag cgctagattc tgtgcgttgt
     7921 tgatttacag acaattgttg tacgtatttt aataattcat ta
//
