LOCUS       Exported                4814 bp ds-DNA     linear   UNA 19-NOV-2020
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   john
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Nov 20, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     complement(53..72)
                     /label=pGL4seqR3
     polyA_signal    364..412
                     /note="synthetic polyadenylation signal"
     misc_feature    426..517
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     466..485
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    562..586
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    587..1061
                     /label=Silent chromatin region-chr9-89982-89508
     protein_bind    complement(1062..1086)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1125..1156
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(1126..1149)
                     /label=pGL4seqR
     regulatory      1183..1192
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1189..2841
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     2729..2748
                     /label=LUCseqF
     polyA_signal    2885..3006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2896..2915)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2950..2969
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3425..4013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3890..3909
                     /label=OriseqF
ORIGIN
        1 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
       61 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
      121 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
      181 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
      241 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
      301 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
      361 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
      421 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
      481 gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc ggtacctgag
      541 ctcgctagcc tcgaggatat caccactttg tacaagaaag ctgggtgaat gtctggagtt
      601 agggtggaag gattatggta aagaaaaaga ttaggcgtta ggactcaaat tagtaaatta
      661 cttcttccag ccaacctaag aagtctggaa tttgtcatag aggttataga gaattcttga
      721 agggttttca ttaggaaagt aacatgatgc gattttcatt gtacaaagat cactctggta
      781 gagagtggat tgagaagaaa aggctggaag aaaggcaagt tgtcaggaaa tgtttgcagc
      841 cattcatagt gaaatgatga atgaaggcag aaacaacaac agggattgag aggacagggt
      901 gtttggaaga catatttttg ctcagttatt cctgtctgag aattggtcct ctcttcctca
      961 gggctgggaa gtccacagcc atctttgtgc tatgtgttcc tgacctgtag ttacagctga
     1021 ttggatcagg gggtacattt gcctgagatg gatcagtcag gagcctgctt ttttgtacaa
     1081 acttgtgata tcaagatctg gcctcggcgg ccaagcttag acactagagg gtatataatg
     1141 gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccat ggaagatgcc
     1201 aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag
     1261 cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac
     1321 gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca
     1381 gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat
     1441 agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca
     1501 gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc
     1561 gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg
     1621 atcatacaaa agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg
     1681 tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag
     1741 agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg
     1801 cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac
     1861 cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac
     1921 cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc
     1981 atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct
     2041 gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac
     2101 gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt
     2161 gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa
     2221 acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag
     2281 gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg
     2341 aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac
     2401 cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc
     2461 tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac
     2521 aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc
     2581 ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc
     2641 gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc
     2701 caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa
     2761 ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag
     2821 ggcggcaaga tcgccgtgta ataattctag agtcggggcg gccggccgct tcgagcagac
     2881 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2941 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3001 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     3061 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc
     3121 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     3181 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     3241 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3301 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3361 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3421 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3541 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3601 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3661 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3721 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3781 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3841 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3901 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3961 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4021 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4081 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4141 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa
     4201 ccactgcagt ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt
     4261 tcgttcgtcc atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt
     4321 accatcaggc cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt
     4381 gtcagcaatg aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc
     4441 cgcctccatc cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag
     4501 tagtttccga agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg
     4561 tatggcttcg ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt
     4621 atgaagaaat gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc
     4681 ggtgttgtcg ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt
     4741 aagatgcttt tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg
     4801 gcgaccaagc tgct
//
