LOCUS       Exported                9479 bp ds-DNA     circular SYN 21-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Nov 22, 2020 from SnapGene 5.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1825..1942
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2024..2248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2295..2315
                     /label=rev106bT
     misc_feature    2320..2340
                     /label=rev106bT
     misc_feature    2347..2367
                     /label=rev106bT
     misc_feature    2372..2392
                     /label=rev106bT
     CDS             complement(2415..2435)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2439..2459)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2463..2483)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2496..3212)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2699..2717)
                     /label=Primer 2
     primer_bind     complement(2720..2743)
                     /label=Probe
     primer_bind     complement(2776..2796)
                     /label=Primer 1
     promoter        complement(3293..3570)
                     /label=chicken beta-actin promoter
     enhancer        3572..3951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4113..4608
                     /label=PGKp
     misc_feature    4215..5336
                     /label=AgeI to XhoI subclone into pgk UTR vector
     CDS             4627..5328
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     5143..5163
                     /label=dtomato sense
     primer_bind     5208..5231
                     /label=probe
                     /note="CTACGTGGACACCAAGCTGGACAT"
     primer_bind     5239..5259
                     
     misc_feature    5339..5927
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5810..5821)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6000..6233
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6305..6426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6466..6601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6622..6640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6650..6666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6808..7263
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7289..7393
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7394..8254
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8425..9013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9301..9322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9337..9367
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9375..9391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9399..9415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9436..9454
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat
     1801 acaaatggca gtattcattc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     1861 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     1921 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggtc
     1981 tagtggatcc tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
     2041 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     2101 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     2161 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     2221 acagatggct ggcaactaga aggcacagtc gaggctgatc agcttatcga caacggcatc
     2281 gctactctag agagatctgc actgtcagca ctttacgata tctgcactgt cagcacttta
     2341 accggtatct gcactgtcag cactttatca catctgcact gtcagcactt tattataagg
     2401 atcctctagg atcctacctt tctcttcttt tttggatcta cctttctctt cttttttgga
     2461 tctacctttc tcttcttttt tggatcagct cgagtcttgt acagctcgtc catgccgaga
     2521 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
     2581 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
     2641 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
     2701 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
     2761 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
     2821 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
     2881 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
     2941 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
     3001 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
     3061 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
     3121 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
     3181 ccggtgaaca gctcctcgcc cttgctcacc atggtggcgg ccgctaccgg ggcgggcggc
     3241 gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc
     3301 gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc
     3361 ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct
     3421 cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc atcgctgcac
     3481 aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga
     3541 gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga
     3601 tgtactgcca agtaggaaag tcccgtaagg tcatgtactg ggcataatgc caggcgggcc
     3661 atttaccgtc attgacgtca atagggggcg gacttggcat atgatacact tgatgtactg
     3721 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     3781 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     3841 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     3901 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgc caacatggcg
     3961 gtcatattgg acatgagcca atataaatgt acatattatg atatagatac aacgtatgca
     4021 atggccaata gccaatattg atttatgcta tataaccaat gaataatatg gctaatggcc
     4081 aatattgaag atcccccggg ctgcaggaat tccagtactt ttcccaaggc agtctggagc
     4141 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     4201 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     4261 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     4321 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     4381 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     4441 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     4501 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     4561 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgaa gcttgccgcc
     4621 ggcaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     4681 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     4741 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     4801 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     4861 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     4921 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     4981 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     5041 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     5101 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     5161 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     5221 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     5281 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta actcgagtaa
     5341 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5401 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5461 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5521 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5581 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5641 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5701 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5761 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5821 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5881 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcagg tacctttaag
     5941 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6001 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6061 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6121 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6181 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6241 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6301 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6361 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6421 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     6481 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     6541 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     6601 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     6661 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6721 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     6781 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     6841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7261 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7621 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9061 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9121 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9181 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9241 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9301 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9361 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9421 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctt
//
