LOCUS       3TERM_NtpsbA_rev_chlo   2470 bp ds-DNA     circular     05-NOV-2020
DEFINITION  .
KEYWORDS    "Plasmid.size:2470" "Insert.length:417" "Vector.length:2053"       
            "Availability:yes" "Status:Available" "Plasmids Box- Number"
            "Glycerol stock" "L0 position" "Type.of.part:3TERM"
            "Description:plastid constructs, Tobacco psbA Term -
            Domesticated-REV" "Phytozome.ID" "TF.family" "MarpolBase.Name"
            "Original.Name:EF356-Tobacco psbA Term - Domesticated-REV-pUAP3"
            "Acceptor.plasmid:pUAP3" "Resistance" "Source of part-DNA"
            "Analysis of plasmid (sequence RE)" "Designer:EF" "Builder:EF"
            "Curator (annotations seq schema):EF / SSG" "Which Type IIS"
            "kit:yes, chloroplast" "Shared.with..Relevant.info"
            "Twist.order.item.id" "Twsit.line.item.number" "Twist.Plate"
            "Twist.Plate.number" "Twist.Well" "Yield.ng" "GENEWIZ.Clone.ID"
            "Genewiz.Order" "Label"
FEATURES             Location/Qualifiers
     misc_feature    complement(2177..2183)
                     /label="SapI_dom"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1781..1781
                     /label="here, keep"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1793..2201
                     /label="Nt psbA terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      complement(772..866)
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(887..1546)
                     /label="CmR (cat, chloramphenicol acetyltransferase)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1789..1792
                     /label="fusion"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2202..2205
                     /label="fusion"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Replication_ori complement(1..589)
                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 TTTCCACAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
       61 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
      121 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
      181 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
      241 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
      301 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
      361 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
      421 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
      481 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
      541 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
      601 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
      661 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
      721 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGC TCGAGGCTTG GATTCTCACC
      781 AATAAAAAAC GCCCGGCGGC AACCGAGCGT TCTGAACAAA TCCAGATGGA GTTCTGAGGT
      841 CATTACTGGA TCTATCAACA GGAGTCCAAG CGAGCTCGAT ATCAAATTAC GCCCCGCCCT
      901 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC
      961 AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT
     1021 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA
     1081 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT
     1141 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA
     1201 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT
     1261 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG
     1321 CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT
     1381 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG
     1441 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC
     1501 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG
     1561 CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT
     1621 GAAAGTTGGA ACCTCTTACG TGCCCGATCA ACTCGAGTGC CACCTGACGT CTAAGAAACC
     1681 ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCAGAA TTTCAGATAA
     1741 AAAAAATCCT TAGCTTTCGC TAAGGATGAT TTCTGGAATT CggtctcTGC TTCTGCAGGA
     1801 TCCTGGCCTA GTCTATAGGA GGTTTTGAAA AGAAAGGAGC AATAATCATT TTCTTGTTCT
     1861 ATCAAGAGGG TGCTATTGCT CCTTTCTTTT TTTCTTTTTA TTTATTTACT AGTATTTTAC
     1921 TTACATAGAC TTTTTTGTTT ACATTATAGA AAAAGAAGGA GAGGTTATTT TCTTGCATTT
     1981 ATTCATGATT GAGTATTCTA TTTTGATTTT GTATTTGTTT AAAATTGTAG AAATAGAACT
     2041 TGTTTCTCTT CTTGCTAATG TTACTATATC TTTTTGATTT TTTTTTTCCA AAAAAAAATC
     2101 AAATTTTGAC TTCTTCTTAT CTCTTATCTT TGAATATCTC TTATCTTTGA AATAATAATA
     2161 TCATTGAAAT AAGAAAGAAG AtCTATATTC GAAGCTTATC GCTCCTgaga ccCTGCAGTC
     2221 CGGCAAAAAA GGGCAAGGTG TCACCACCCT GCCCTTTTTC TTTAAAACCG AAAAGATTAC
     2281 TTCGCGTTAT GCAGGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
     2341 GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG
     2401 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
     2461 TGCTGGCGTT
//