LOCUS       L1_358-Ck4-chlo_(HS2-R- 8005 bp ds-DNA     circular     20-NOV-2020
DEFINITION  .
KEYWORDS    "Acceptor plasmid/ assembly description" "Analysis of plasmid      
            (sequence, RE)" "Availability" "Builder:EF" "Concentration (ng/ul)"
            "Curator (annotations, seq, schema):EF / SSG" "Description"
            "Designer:EF" "Glycerol stock" "Insert length" "Original name"
            "Plasmid size, bp:8005" "Plasmids Box- Number" "Resistance" "Source
            of part-DNA" "Vector length" "Which Type IIS" "kit:yes,
            chloroplast"
FEATURES             Location/Qualifiers
     misc_feature    484..2048
                     /label="rbcL HS right"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(4808..5808)
                     /label="pVS1-Rep\ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      complement(6618..6898)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(3214..4214)
                     /label="STA\region\from\pVS1\plasmid"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    433..458
                     /label="LB"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(7193..8005)
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2128..2152
                     /label="RB"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2112..2114
                     /label="GGT fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(6218..6478)
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    476..478
                     /label="CAG fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1579..1581
                     /label="premature stop"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcacagag
      481 gagATTGTTC TATTATTAAA TTACTTAAAT TTTAAATTTT TCATATATGT TTTAAAAATA
      541 ATTATATATA AAAATATGGA ACATTTTTTT TATAATTATT TTCTCTTAAA ATTTTAAAAA
      601 ATTTATAAAA AAAGAAATAA AGAGGATTTT TTTATGTCTT TAATGAATTG GTTTGAAGAT
      661 AAACGAAGAT TTGGTGGATT AATTGGCGCT TTTATTGAAA AAGCTACTAA AGGATATATT
      721 TTTAGTGAAA GAGAAAAAGA TCGATATATT AAAATTGACA CTACTAAGGG ATTATGGACT
      781 AGATGTGACA ATTGTGAAAA TATGTTATAT GTTAGATTTT TGAGACAAAA TAAACGAATT
      841 TGCGAAGAAT GCGGATATCA TTTGCAAATG AGTAGTACAG AAAGAATTGA ACTTTTAATT
      901 GATCGCGGGA CTTGGTATCC AATGGATGAA GATATGACTG CTCGAGATGT TCTTAAATTT
      961 TCTGATGAAG ATTCTTATAA AAATCGAATT GCTTTTTATC AAAAAAGAAC TGGTTTAACT
     1021 GATGCAATTC AAACAGGCAT AGGTCAATTA AACGGTATTC CTATTGCATT GGGAGTAATG
     1081 GATTTTCAAT TTATGGGCGG TAGTATGGGA TCTGTAGTAG GTGAAAAAAT TACTCGTCTT
     1141 ATTGAATATG CTACTAGAGC ATCAATGCCA CTAATTATAG TATGTTCTTC TGGTGGAGCA
     1201 CGCATGCAAG AAGGAACATT AAGTTTAATG CAAATGGCTA AAATTTCTTC GGTTTTGCAA
     1261 ATTCATCAAG CCCAAAAAAG ATTACTTTAT ATAGCTATTC TTACCTATCC TACAACAGGA
     1321 GGAGTTACTG CAAGTTTTGG TATGTTAGGG GATATTATTA TTGCTGAGCC AAAAGCTTAT
     1381 ATTGCATTTG CAGGAAAAAG AGTTATTGAA CAAACTTTAC GACAAAAAAT ACCAGATGGT
     1441 TTTCAAGTTG CAGAATCATT ATTTGATCAT GGTTTACTTG ATTTAATTGT TCCACGAAAT
     1501 CTTTTAAAAG GTGTTTTAAG TGAAATTTTT GAATTATATA ATGCTGCTCC GTGTAAAAAA
     1561 TTTCAAAATT CTTTTTTTTA ATAATTTTGT TAGATTTTTA GTAGTTTTTT TTAATTCAAA
     1621 TTTTTTATAA ATTTTTATAT TTTGTTATTG TTATTTATTA AAATAAGATA TAATTTTTAT
     1681 TTAGTTTAGT TTTAAATAAA ATATAGTATA TATTATATTT AtttTTTTTT TTTATTGTAT
     1741 TTTAAGGTAT TTTTTATGAC AGCTTCTTAT TTACCTTCTA TTTTTGTTCC TTTAGTTGGA
     1801 TTAATTTTTC CTGCTATTAC TATGGCTTCA TTATTTATAT ATATTGAACA AGATGAAATT
     1861 TTATAAATTA ATTGGAGATT AAAATTTTTT TTTACTTTTT ACAAAATATA TATATGTTAT
     1921 AGATCAATTT TTTTTTGATA TATATTTTAT AATACTTTGT GAAAAGTAAA AAATTTTTTT
     1981 TGAATTTATT GTTATTATAA TATTGATTAT TTTTTAATAG AAAAATAATC AATATTTTTT
     2041 TTATCAACAT TATTATTATT CAACTAATTT AGGAGACATT CTTTTGTTAT GAATTTACAA
     2101 GTGGACCGCT aggttgaaga gcaattctga caggatatat tggcgggtaa acctaagaga
     2161 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     2221 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     2281 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt catctgaaaa
     2341 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     2401 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     2461 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     2521 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     2581 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     2641 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     2701 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     2761 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     2821 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     2881 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     2941 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     3001 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     3061 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     3121 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     3181 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     3241 ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     3301 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     3361 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     3421 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     3481 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     3541 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     3601 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     3661 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     3721 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     3781 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     3841 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     3901 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     3961 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     4021 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     4081 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     4141 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     4201 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     4261 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     4321 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     4381 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     4441 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     4501 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     4561 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     4621 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     4681 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     4741 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     4801 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     4861 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     4921 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     4981 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     5041 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     5101 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     5161 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     5221 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     5281 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     5341 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     5401 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     5461 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     5521 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     5581 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     5641 actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     5701 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     5761 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     5821 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag tgctgcaaaa
     5881 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     5941 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     6001 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
     6061 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc acagcttgtc
     6121 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6181 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     6241 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6301 atgcgtaagg agaaaatacc gcatcaggcg ctcatccgct tcctcgctca ctgactcgct
     6361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     6961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7141 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat tagaaaaact
     7201 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     7261 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     7321 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     7381 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     7441 agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     7501 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     7561 gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     7621 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     7681 cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     7741 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     7801 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     7861 catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag
     7921 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag
     7981 acgtttcccg ttgaatatgg ctcat
//