LOCUS       pCk1chlo                3077 bp ds-DNA     circular     20-NOV-2020
DEFINITION  .
KEYWORDS    "Builder:EF" "Concentration (ng/ul)" "Curator (annotations, seq,   
            schema):EF /  SSG" "Description" "Designer:EF" "Original name"
            "Plasmid size, bp:3077" "Plasmids Box- Number" "Plate number"
            "Resistance:Kan" "Which Type IIS" "kit:yes, chloroplast"
FEATURES             Location/Qualifiers
     misc_feature    complement(1035..1040)
                     /label="BasI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1625..1628
                     /label="CGCT fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(1470..1490)
                     /label="lac\operator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1926..2186)
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1630..1632
                     /label="GCA fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1634..1640)
                     /label="SapI"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(2207..2821)
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(534..557)
                     /label="EF446-pcsa-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1618..1623
                     /label="BasI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          830..859
                     /label="EF086-LCK1_F2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    1018..1024
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          1655..1676
                     /label="EF446-pcsa-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(join(2901..3077,1..636))
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1131..1452)
                     /label="LacZ"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1030..1033
                     /label="GGAG fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1026..1028
                     /label="ATG fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1497..1526)
                     /label="lac\promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(2326..2606)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
       61 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
      121 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
      181 cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
      241 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      301 ttcttctaat acctggaatg ctgttttccc tgggatcgca gtggtgagta accatgcatc
      361 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      421 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      481 caactctggc gcatcgggct tcccatacaa tcggtagatt gtcgcacctg attgcccgac
      541 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      601 ccttgagcaa gacgtttccc gttgaatatg gctcataaca gaacttatta tttccttcct
      661 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
      721 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
      781 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
      841 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
      901 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
      961 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgaattcg cggccgcgct
     1021 cttcaatgag gagagagacc cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     1141 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     1201 agaaaatacc gcatcaggcg ctcatccgcc attcaggctg cgcaactgtt gggaagggcg
     1261 atcggcgcag gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     1321 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     1381 attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca agcttggcgt
     1441 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     1501 tacgagccgg aagcatatag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     1561 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctggggt
     1621 ctctcgctag catgaagagc gcggccgcct gcagtggccg ctcaaaaatg gctggcctac
     1681 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     1741 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1801 cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1861 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     1921 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     1981 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     2041 catccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2101 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2161 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2221 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2281 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2341 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2401 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2461 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2521 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2581 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2641 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2701 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2761 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2821 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2881 tcatgcattc taggtgatta gaaaaactca tcgagcatca aatgaaactg caatttattc
     2941 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     3001 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     3061 ccaacatcaa tacaacc
//