LOCUS       pCk3chlo                3077 bp ds-DNA     circular     20-NOV-2020
DEFINITION  .
KEYWORDS    "Builder:EF" "Concentration (ng/ul)" "Curator (annotations, seq,   
            schema):EF / SSG" "Description" "Designer:EF" "Original name"
            "Plasmid size, bp:3077" "Plasmids Box- Number:Box 10" "Plate
            number" "Resistance:Kan" "Which Type IIS" "kit"
FEATURES             Location/Qualifiers
     misc_feature    complement(861..890)
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(399..404)
                     /label="BasI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1290..1550)
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    394..397
                     /label="GGAG fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(2265..3077)
                     /label="KanR"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(834..854)
                     /label="Lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    994..996
                     /label="CAG fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    390..392
                     /label="TAC fusion site"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    982..987
                     /label="BasI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(495..816)
                     /label="LacZ"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1571..2185)
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    989..992
                     /label="CGCT fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2975..2998)
                     /label="EF446-pcsa-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    382..388
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(1690..1970)
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          1019..1040
                     /label="EF446-pcsa-F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          194..223
                     /label="EF086-LCK1_F2"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(998..1004)
                     /label="SapI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatgaa ttcgcggccg cgctcttcat acaggagaga gacccacagc ttgtctgtaa
      421 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
      481 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
      541 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcatc cgccattcag
      601 gctgcgcaac tgttgggaag ggcgatcggc gcaggcctct tcgctattac gccagctggc
      661 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      721 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga
      781 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      841 atccgctcac aattccacac aacatacgag ccggaagcat atagtgtaaa gcctggggtg
      901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      961 gaaacctgtc gtgccagctg gggtctctcg ctacagtgaa gagcgcggcc gcctgcagtg
     1021 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     1081 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     1141 gacggtgaaa acctctgaca catgcagctc ccggtgacgg tcacagcttg tctgtaagcg
     1201 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1261 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1321 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1381 ggagaaaata ccgcatcagg cgctcatccg cttcctcgct cactgactcg ctgcgctcgg
     1441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2221 aaaactcacg ttaagggatt ttggtcatgc attctaggtg attagaaaaa ctcatcgagc
     2281 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2341 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2401 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     2461 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2521 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2581 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
     2641 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2701 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2761 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2821 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2881 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2941 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     3001 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
     3061 cgttgaatat ggctcat
//