LOCUS       pCsEchlo                3405 bp ds-DNA     circular     20-NOV-2020
DEFINITION  .
KEYWORDS    "Builder:EF" "Concentration (ng/ul)" "Curator (annotations, seq,   
            schema):EF / SSG" "Description" "Designer:EF" "Original
            name:pCsEchlo-LacZ" "Plasmid size, bp:3405" "Plasmids Box- Number"
            "Plate number" "Resistance:Sm" "Which Type IIS:Loop 2.0 (no BsaI,
            SapI)" "kit"
FEATURES             Location/Qualifiers
     misc_feature    complement(1473..1794)
                     /label="LacZ"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1971..1974
                     /label="CGCT fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    814..1074
                     /label="pBR322"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1812..1832)
                     /label="Lac operator"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      394..674
                     /label="pBR322\ori"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1360..1365
                     /label="BasI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(1839..1868)
                     /label="Lac promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2363..2496
                     /label="Spm\Promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    179..793
                     /label="rep\(pMB1)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1976..1981)
                     /label="BasI"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    190..778
                     /label="ori"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1367..1370
                     /label="GCTT fusion site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1959..1965
                     /label="SapI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1982..1989
                     /label="NotI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1376..1382)
                     /label="SapI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(1990..1990)
                     /label="here, keep"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             join(2497..3405,1..102)
                     /label="SpmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1352..1359
                     /label="NotI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
       61 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatcacctag aatgcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      901 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggatga
      961 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     1021 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
     1081 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
     1141 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtcac cgggagctgc
     1201 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
     1261 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
     1321 taggccagcc atttttgagc ggccaCTGCA GGCGGCCGCg gtctcagctt aatgtgaaga
     1381 gccacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1441 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1501 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     1561 cgctcatccg ccattcaggc tgcgcaactg ttgggaaggg cgatcggcgc aggcctcttc
     1621 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1681 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
     1741 gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
     1801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcatat
     1861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1921 tgcccgcttt ccagtcggga aacctgtcgt gccagctggc tcttcaggta cgcttgagac
     1981 cGCGGCCGCG AATTCatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     2041 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     2101 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     2161 tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     2221 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     2281 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     2341 gaaggaaata ataagttctg ttccagccag gacagaaatg cctcgacttc gctgctaccc
     2401 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa
     2461 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     2521 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     2581 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     2641 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     2701 acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     2761 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     2821 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     2881 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     2941 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     3001 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     3061 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     3121 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     3181 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     3241 ccgcccgact gggctggtga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     3301 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     3361 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggct
//