LOCUS       a3_EM_pEZT              9089 bp    DNA     circular     24-NOV-2020
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13620321|
COMMENT     VNTDBDATE|-13620321|
COMMENT     LSOWNER|
COMMENT     VNTNAME|a3_EM_pEZT|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    8573..8813
                     /vntifkey="21"
                     /label=SV40\PolyA
     promoter        complement(5025..5146)
                     /vntifkey="29"
                     /label=p10\Promoter
     misc_feature    689..1549
                     /vntifkey="21"
                     /label=AmpR
     misc_feature    complement(3992..4274)
                     /vntifkey="21"
                     /label=HSV\tk\PolyA
     misc_feature    complement(2902..3435)
                     /vntifkey="21"
                     /label=GentamicinR
     misc_feature    7948..8539
                     /vntifkey="21"
                     /label=WPRE
     promoter        5190..6211
                     /vntifkey="29"
                     /label=CMV+intron
     misc_feature    complement(4294..5007)
                     /vntifkey="21"
                     /label=EGFP
     misc_feature    6265..7935
                     /vntifkey="21"
                     /label=a3\EB
     misc_feature    7399..7716
                     /vntifkey="21"
                     /label=Bril
BASE COUNT     2098 a      2346 c      2261 g      2384 t 
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg 
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc 
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc 
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac 
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga 
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg 
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt 
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc 
     4261 tcccccatct cccggttccg catgctatgc atccttgtac agctcgtcca tgccgagagt 
     4321 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc 
     4381 tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc 
     4441 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt 
     4501 gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac 
     4561 gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa 
     4621 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc 
     4681 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc 
     4741 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca 
     4801 ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt 
     4861 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga 
     4921 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc 
     4981 ggtgaacagc tcctcgccct tgctcaccgg gtgatcaagt cttcgtcgag tgattgtaaa 
     5041 taaaatgtaa tttacagtat agtattttaa ttaatataca aatgatttga taataattct 
     5101 tatttaacta taatatattg tgttgggttg aattaaaggt ccgtatactc cggaatatta 
     5161 ataggatccc ggtccgaagc gcgcggaatt caatattggc cattagccat attattcatt 
     5221 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat 
     5281 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttggca ttgattattg 
     5341 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 
     5401 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 
     5461 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 
     5521 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 
     5581 ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 
     5641 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 
     5701 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg 
     5761 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 
     5821 cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg cggtaggcgt 
     5881 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cactagaagc 
     5941 tttattgcgg tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca 
     6001 gtctcgaact taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt 
     6061 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg 
     6121 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt 
     6181 gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag 
     6241 gctagcctcg aggcggccgc caccatgggc tctggcccgc tctcgctgcc cctggcgctg 
     6301 tcgccgccgc ggctgctgct gctgctgctg ctgtctctgc tgccagtggc cagggcctca 
     6361 gaggctgagc accgtctatt tgagcggctg tttgaagatt acaatgagat catccggcct 
     6421 gtagccaacg tgtctgaccc agtcatcatc catttcgagg tgtccatgtc tcagctggtg 
     6481 aaggtggatg aagtaaacca gatcatggag accaacctgt ggctcaagca aatctggaat 
     6541 gactacaagc tgaagtggaa cccctctgac tatggtgggg cagagttcat gcgtgtccct 
     6601 gcacagaaga tctggaagcc agacattgtg ctgtataaca atgctgttgg ggatttccag 
     6661 gtggacgaca agaccaaagc cttactcaag tacactgggg aggtgacttg gatacctccg 
     6721 gccatcttta agagctcctg taaaatcgac gtgacctact tcccgtttga ttaccaaaac 
     6781 tgtaccatga agttcggttc ctggtcctac gataaggcga aaatcgatct ggtcctgatc 
     6841 ggctcttcca tgaacctcaa ggactattgg gagagcggcg agtgggccat catcaaagcc 
     6901 ccaggctaca aacacgacat caagtacaac tgctgcgagg agatctaccc cgacatcaca 
     6961 tactcgctgt acatccggcg cctgcccttg ttctacacca tcaacctcat catcccctgc 
     7021 ctgctcatct ccttcctcac tgtgctcgtc ttctacctgc cctccgactg cggtgagaag 
     7081 gtgaccctgt gcatttctgt cctcctctcc ctgacggtgt ttctcctggt gatcactgag 
     7141 accatccctt ccacctcgct ggtcatcccc ctgattggag agtacctcct gttcaccatg 
     7201 atttttgtaa ccttgtccat cgtcatcacc gtcttcgtgc tcaacgtgca ctacagaacc 
     7261 ccgacgacac acacaatgcc ctcatgggtg aagactgtat tcttgaacct gctccccagg 
     7321 gtcatgttca tgaccaggcc aacaagcaac gagggcaacg ctcagaagcc gaggcccctc 
     7381 tacggtgccg agctctcagc agatttggag gataactggg agacactgaa cgataacctg 
     7441 aaggttattg aaaaggccga taatgccgcc caggtcaagg acgccctcac caaaatgcgg 
     7501 gccgctgccc tcgatgccca gaaggccact cctcccaagc tggaggataa gagccccgac 
     7561 tccccagaga tgaaggactt tagacacggc ttcgacatcc ttgtggggca gattgacgac 
     7621 gcactgaaac tcgcaaacga gggaaaagtg aaggaagctc aggccgctgc agagcagctg 
     7681 aagacaacca ggaacgccta catccaaaag tacctgctct ctgctttgtc accagaaatc 
     7741 aaagaagcca tccaaagtgt caagtatatt gctgaaaata tgaaagcaca aaatgaagcc 
     7801 aaagagattc aagatgattg gaagtatgtt gccatggtga ttgatcgtat ttttctgtgg 
     7861 gttttcaccc tggtgtgcat tctagggaca gcaggattgt ttctgcaacc cctgatggcc 
     7921 agggaagatg cataaggtac caagcttaat caacctctgg attacaaaat ttgtgaaaga 
     7981 ttgactggta ttcttaacta tgttgctcct tttacgctat gtgaatacgc tgctttaatg 
     8041 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     8101 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     8161 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     8221 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     8281 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     8341 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     8401 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     8461 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     8521 tgggccgcct ccccgcctgc agtctcgaca agcttgtcga gaagtactag aggatcataa 
     8581 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 
     8641 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 
     8701 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 
     8761 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac 
     8821 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca 
     8881 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct 
     8941 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc 
     9001 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca 
     9061 tgtgacaaac cgtcatcttc ggctacttt 
//
