LOCUS       Exported                4877 bp ds-DNA     circular SYN 25-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Baohui
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 25, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          277..775
                     /organism="Homo sapiens"
                     /chromosome="1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     source          2281..2763
                     /organism="Homo sapiens"
                     /chromosome="1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     misc_feature    253..276
                     /label=LMNA-sgRNA
     misc_feature    277..775
                     /label=LMNA-5HA
     misc_feature    776..796
                     /label=5 linker
     misc_feature    797..1132
                     /label=mTagBFP-exon 1
     misc_feature    1133..1896
                     /label=intron
     misc_feature    1143..1157
                     /label=Feature 10
     misc_feature    1158..1169
                     /label=BstXI
     misc_feature    1170..1190
                     /label=Feature 50
     misc_feature    1185..1207
                     /label=MS2V5-1
     misc_feature    1212..1233
                     /label=sgTS1
     misc_feature    1238..1260
                     /label=MS2V5-2
     misc_feature    1265..1286
                     /label=sgTS1
     misc_feature    1291..1313
                     /label=MS2V5-3
     misc_feature    1318..1339
                     /label=sgTS1
     misc_feature    1344..1366
                     /label=MS2V5-4
     misc_feature    1371..1392
                     /label=sgTS1
     misc_feature    1397..1419
                     /label=MS2V5-5
     misc_feature    1424..1445
                     /label=sgTS1
     misc_feature    1450..1472
                     /label=MS2V5-6
     misc_feature    1466..1476
                     /label=Feature 51
     misc_feature    1477..1498
                     /label=sgTS1
     misc_feature    1503..1525
                     /label=MS2V5-7
     misc_feature    1530..1551
                     /label=sgTS1
     misc_feature    1556..1578
                     /label=MS2V5-8
     misc_feature    1583..1604
                     /label=sgTS1
     misc_feature    1609..1631
                     /label=MS2V5-9
     misc_feature    1636..1657
                     /label=sgTS1
     misc_feature    1662..1684
                     /label=MS2V5-10
     misc_feature    1689..1710
                     /label=sgTS1
     misc_feature    1715..1737
                     /label=MS2V5-11
     misc_feature    1742..1763
                     /label=sgTS1
     misc_feature    1764..1780
                     /label=Feature 53
     misc_feature    1768..1790
                     /label=MS2V5-12
     misc_feature    1795..1816
                     /label=sgTS1
     misc_feature    1821..1832
                     /label=BstXI
     misc_feature    1897..2259
                     /label=from 1209
     misc_feature    1897..2259
                     /label=mTagBFP-exon 2
     misc_feature    2260..2280
                     /label=3 linker
     misc_feature    2281..2763
                     /label=LMNA-3HA
     gene            2281..2763
                     /gene="LMNA"
                     /gene_synonym="CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD;
                     FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; 
                     PRO1"
                     /label=LMNA
                     /note="lamin A/C; Derived by automated computational 
                     analysis using gene prediction method: BestRefSeq,Gnomon."
                     /db_xref="GeneID:4000"
                     /db_xref="HGNC:HGNC:6636"
                     /db_xref="HPRD:01035"
                     /db_xref="MIM:150330"
     CDS             2281..2633
                     /codon_start=1
                     /gene="LMNA"
                     /gene_synonym="CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD;
                     FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; 
                     PRO1"
                     /product="lamin isoform A"
                     /label=LMNA
                     /note="isoform A is encoded by transcript variant 1; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS1129.1"
                     /db_xref="GeneID:4000"
                     /db_xref="HGNC:HGNC:6636"
                     /db_xref="HPRD:01035"
                     /db_xref="MIM:150330"
                     /protein_id="NP_733821.1"
                     /translation="ETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYI
                     DRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLE
                     LSKVREEFKELKA"
     misc_feature    2281..2295
                     /label=sgRNA targeting sequence
     misc_feature    2295
                     /label=g to A mutation
     misc_feature    2296..2298
                     /label=PAM cgg to cga
     primer_bind     complement(2325..2344)
                     /label=SEQ.HYP168
     misc_feature    2764..2787
                     /label=LMNA-sgRNA
     rep_origin      complement(3125..3710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3881..4741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4742..4846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacaga
      241 gaccgcggcc gcccgctggg acggggtctc catggcccct ccttggctct gccctctagc
      301 ccagaaggtc tgaggcaatg ggggcaagct tggagccgac agtgctgagc aggcaggagc
      361 caagagaggg gaagcttgag cctcacgcag ttaggggtgc gctggagagg gtggggcccg
      421 actccgccac accccaacgg tccttccccc tcctcaccac tcccgccccc acccccaatg
      481 gatctgggac tgccccttta agagtagtgg cccctcctcc cttcagagga ggacctatta
      541 gagcctttgc cccggcgtcg gtgactcagt gttcgcggga gcgccgcacc tacaccagcc
      601 aacccagatc ccgaggtccg acagcgcccg gcccagatcc ccacgcctgc caggagcaag
      661 ccgagagcca gccggccggc gcactccgac tccgagcagt ctctgtcctt cgacccgagc
      721 cccgcgccct ttccgggacc cctgccccgc gggcagcgct gccaacctgc cggccggatc
      781 gggtgggact agtggcatga gcgagctgat taaggagaac atgcacatga agctgtacat
      841 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
      901 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
      961 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
     1021 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     1081 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acgtaagtat
     1141 gaaattcagg gatacggcca ccttgttggc cggtaaccta caaacgggtg gaggatcacc
     1201 ccacccgaca cgcaccgatg ctctccgagg aggcacaagg gtggaggaac accccaccct
     1261 ccaggcaccg atgctctccg aggaggcaaa cagaagcacc atcagggctt ctgctacgca
     1321 ccgatgctct ccgaggagga acaaggaatc accatcaggg attccctgtg gcaccgatgc
     1381 tctccgagga ggacgacggg aggacgatca cgcctcccga atagcaccga tgctctccga
     1441 ggaggtcagt gcgtggagca tcagcccacg cagccagcac cgatgctctc cgaggaggct
     1501 ttcgcgaaga gcatcagcct tcgcgccatg caccgatgct ctccgaggag gcctacagga
     1561 acagcatcag cgttcctgcc cagcaccgat gctctccgag gaggtgtccc catgcagcat
     1621 cagcgcatgg gccccgcacc gatgctctcc gaggaggcac atccgagcac caacagggct
     1681 cggagtgtgc accgatgctc tccgaggagg accctccatg gaccatcagg ccatggactc
     1741 tgcaccgatg ctctccgagg aggcttctcg aagcagcatc agcgcttcga aacagcaccg
     1801 atgctctccg aggaggttct ccaccttgtt ggcatatttg ccaaatagtg gaaatgtgaa
     1861 gtactgacaa aacttttccc tttttcaatc taatagggct gcctcatcta caacgtcaag
     1921 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1981 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     2041 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     2101 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     2161 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     2221 tactgcgacc tccctagcaa actggggcac aagcttaatg gaaccggtgc tggaagtggt
     2281 gagaccccgt cccaacgacg cgccacccgc agcggggcgc aggccagctc cactccgctg
     2341 tcgcccaccc gcatcacccg gctgcaggag aaggaggacc tgcaggagct caatgatcgc
     2401 ttggcggtct acatcgaccg tgtgcgctcg ctggaaacgg agaacgcagg gctgcgcctt
     2461 cgcatcaccg agtctgaaga ggtggtcagc cgcgaggtgt ccggcatcaa ggccgcctac
     2521 gaggccgagc tcggggatgc ccgcaagacc cttgactcag tagccaagga gcgcgcccgc
     2581 ctgcagctgg agctgagcaa agtgcgtgag gagtttaagg agctgaaagc gcggtgagtt
     2641 cgcccaggtg gctgcgtgcc tggcggggag tggagagggc ggcgggccgg cgcccctggc
     2701 cggccgcagg aagggagtga gagggcctgg aggccgataa ctttgccata gtctcctccc
     2761 tccgccatgg agaccccgtc ccagcggccc gggcgacgga tggtgatccc cctggccagt
     2821 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     2881 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
     2941 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     3001 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg acggtctcga
     3061 ctcacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3541 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3781 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3841 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3901 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3961 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4021 gagaaccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4081 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4141 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4201 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4261 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4321 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4381 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4441 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4501 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4561 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4621 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4681 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4741 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4801 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4861 aagtgccacc tgacgtc
//
