LOCUS       Exported                8063 bp ds-DNA     circular SYN 02-DEC-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 2, 2020 from SnapGene Viewer 5.2.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pAAV_EF1a_df_R133C_M2V1HaloRev_WPRE_HGHp
                     A"
     misc_feature    1..34
                     /label=LoxP
                     /note="LoxP"
     misc_feature    283..1453
                     /label=EF1a Promoter
                     /note="EF1a Promoter"
     misc_feature    1484..1517
                     /label=LoxP
                     /note="LoxP"
     misc_feature    1568..1601
                     /label=LoxP
                     /note="LoxP"
     misc_feature    complement(1627..4041)
                     /label=Mecp2V1-HaloTag
                     /note="Mecp2V1-HaloTag"
     misc_feature    complement(2515..2538)
                     /label=Linker
                     /note="Linker"
     misc_feature    4048..4081
                     /label=LoxP
                     /note="LoxP"
     misc_feature    4132..4165
                     /label=LoxP
                     /note="LoxP"
     misc_feature    4192..4779
                     /label=WPRE
                     /note="WPRE"
     misc_feature    4808..5286
                     /label=hGH pA Terminator
                     /note="hGH pA Terminator"
ORIGIN
        1 ataacttcgt ataaagtatc ctatacgaag ttatcctgca ggcagctgcg cgctcgctcg
       61 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      121 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
      181 cgcacgcgta agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct
      241 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      301 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      361 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      421 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      481 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      541 tctttacggg ttatggccct tgcgtgcctt gaattacttc cactggctgc agtacgtgat
      601 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      661 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      721 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      781 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
      841 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
      901 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
      961 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     1021 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     1081 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     1141 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     1201 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     1261 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1321 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1381 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1441 tcaggtgtcg tgaggtaccg gatcctctag agtcgactcc ggaataactt cgtataggat
     1501 actttatacg aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa
     1561 cctcttaata acttcgtata gcatacatta tacgaagtta tggcgcgcct tacttgtaca
     1621 catgtcctaa ccggaaatct cgagggtaga cagccagcgc gcgatctcgc tgccgatcag
     1681 gtccgggttg tcttcttgca gcagattcag acccgggccg atgtccacag ccttgcagtt
     1741 aggcaggctt ttggccaggc gagcggcttc ggccggtggg atcagaacgc ctggggtgcc
     1801 ccagaacagc agcttcggga caggggactg gtgcagccag tccatgtatt cttcgaccag
     1861 cgcgacgatg ttcgctggct caccggcgat tggcagctcg tttgggaagc gccacagtgg
     1921 ctcgcggtca acaggattca ggaacggctc gcggtaatgg tccatctcga cttcagtcag
     1981 cgggcggacg acacccatcg gcagcgtacc ctcgataaaa acgttctgat cgatgatcag
     2041 cttgcggccg acgtcggtgg tgcggaaggc ctggaaggtc tcgcgggcaa attctggcca
     2101 ttcgtcccag gtcgggatag ggcggatgaa ctccataaat gcaatacctt tgacgcgctc
     2161 tggattgcgc ttggcccagt ggaaacccag agcggagccc cagtcgtgaa tgaccaggac
     2221 gacctcttcc agacccaggg cttcgatgaa ggcatccatg aagcggacgt ggtcgtcgaa
     2281 gaaataaccc aggtctggtt tgtcggattt gcccataccg atcaggtctg gagcaatgca
     2341 gcgatgggtc ggtgcaacat gcgggatgat gttgcgccac acgtaggagg aggtcgggtt
     2401 accgtgcagg aacagcacag gggtgccatc gcgcggacca acatcgacgt agtgcatgcg
     2461 ctcgcccagg acttccacat aatgggggtc gaatggaaag ccagtaccga tttcggtggc
     2521 gaccggtgga tccttacagc taactctctc ggtcacgggc gtccggctgt ccacaggctc
     2581 ctctctgttt ggccttggca tggaagatga aacaatgtct ttgcgctctc cctcccctcg
     2641 gtgtttgtac ttttctgcaa ctgtggtagt ggtggcgacc ataggctgag tcttagctgg
     2701 ctccttgggg cagccatcgc tttccagtga gcctcctcgg ggcatcttct cttctttgca
     2761 gatgctgctg ctcaagtcct gaggctcagg ggggctgatg gggtcctcag agctctcagg
     2821 ctcaggtggg ggtggggatg ggagcagtgg catgggggcc tttgtggact ctgagtgatg
     2881 gtggtgatga tggtgctcct tcttaggtgg ggaggaggca ctgctgctgc gccccttggg
     2941 gctgctctcc ttgcttttac gcccagggct cttgcaggtc ttcagtccct tcccgctttt
     3001 ctcaccaagg gtggacacca gcaggggctt caccacttcc ttgacctcga tgctgaccgt
     3061 ctcccgggtc ttgcgcttct tgatggggag cacagtctca tgcacagacc gtatggaaga
     3121 ctccttcacg gctttctttt tggcctcagc tgcagcagct gccaccacac tcccaggctt
     3181 tctaccccgt ttcttaggaa tggcctgggg gtcagcttca gcttttcgct ttctgccagg
     3241 gcgtttgatc accatgacct gggcagatgt ggtagcccca cctccctcac ccttaccccc
     3301 aggcgatgct tggaaaggca tcttgacaac aagtttccca gggctcttct ccaggaccct
     3361 tttcacctga acaccttctg atgctgctgc ctttggtctc ccagtgccgc tccctttggg
     3421 gcgtccccga cccctgccag ttcctggagc tttgggagat ttgggcttct taggtggttt
     3481 ctgctctctc ctggaggggc tccctctccc agttaccgtg aagtcaaaat cattagggtc
     3541 caaggaggtg tctcccacct tttcaaagta tgcaatcaat tctactttag agcaaaaagc
     3601 ttttccctgg ggattgatca aatatacatc atactttcca gcagatcggc cagacttcct
     3661 ttgtttaagc tttcgtgtcc aaccttcagg caaggtgggg tcatcataca taggtccccg
     3721 gtcacggata atggagcgcc gctgtttggg ggaagccgag gcttctggca ctgctggggc
     3781 agagcctgag ctttctgatg tttctgcttt gcctgcctct gctggctctg cagaatggtg
     3841 ggctgaaggt tgtagtggct catgcttgcc ttctttgtcc tccttcttgt ctttcttcgc
     3901 cttcttaaac ttcagtggct tgtctctgag gccctggaga tcctggtctt ctgacttttc
     3961 ctccagtctc tcctcctcgc ctcctcctcc tcctccgctc ggcgcggcgg cggcggcggc
     4021 ggtggcggca gcggcggcca tgctagcata acttcgtata aagtatccta tacgaagtta
     4081 tttgccttaa cccagaaatt atcactgtta ttctttagaa tggtgcaaag aataacttcg
     4141 tataatgtat gctatacgaa gttatgaatt cgatatcaag cttatcgata atcaacctct
     4201 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     4261 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4321 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4381 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4441 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4501 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4561 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctatgttgc
     4621 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4681 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4741 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag cgctgctcga
     4801 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4861 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4921 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4981 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     5041 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     5101 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     5161 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     5221 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     5281 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
     5341 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     5401 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     5461 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5521 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5581 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5641 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5701 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     5761 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5821 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5881 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5941 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     6001 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6061 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6121 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6181 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     6241 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6361 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6421 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6481 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6541 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6601 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6661 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6721 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6781 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6841 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     6901 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     6961 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7021 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7081 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7141 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7201 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7561 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8041 ttgctggcct tttgctcaca tgt
//
