LOCUS       AAVS1_puro_CAG_HexaPro.xdna    11091 bp      DNA     circular   2020-11-29
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : HexaPro.xdna [3121  nt] : (KpnI[1750] / MluI[4
            875]) to AAVS1_puro_CAG_nCoV-2P-F3CH2S.xdna [7959  nt] : (Ml
            uI[7155] / KpnI[4030])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             7789..7812
                     /label=8xHisTag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4042..7665
                     /label=Prefusion S ectodomain HexaPro (AA 1-1208) (MN908947)
                     /SerialCloner_Color=&h21FE06
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    7672..7752
                     /label=T4 Foldon
                     /SerialCloner_Color=&hFF80FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    6085..6096
                     /label=[S1/S2] Mutant Furin cleavage site "GSAS" (AA 682-685)
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="GSAS" \ 
     misc_feature    6997..7002
                     /label=Pro-Pro stabilization (AA986-987)
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PP" \ 
     misc_feature    5029..5604
                     /label=RBD (AA 330-521)
                     /SerialCloner_Color=&hFB027F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="PNITNLCPFGEV" \ 
     misc_feature    4087..4956
                     /label=NTD 
                     /SerialCloner_Color=&hCCCCCC
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="VNLTTR" \ 
     misc_feature    6487..6540
                     /label=Fusion peptide (FP)
                     /SerialCloner_Color=&h20FEFE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="SFIEDLLFNKVTLADAGF" \ 
     misc_feature    6481..6486
                     /label=S2'
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="KR" \ 
     misc_feature    6763..6996
                     /label=HR1 Heptad Repeat 1
                     /SerialCloner_Color=&hFFFE0A
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="GIGVTQN" \ 
     misc_feature    7003..7146
                     /label=CH Central Helix
                     /SerialCloner_Color=&hFDCC65
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="EAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLG" \ 
     misc_feature    7267..7464
                     /label=CD Connector domain
                     /SerialCloner_Color=&hCC66FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="TTAPAI" \ 
     misc_feature    7462..7464
                     /label=L1141
                     /SerialCloner_Color=&hCC66FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="L" \ 
     misc_feature    7555..7644
                     /label=HR2
                     /SerialCloner_Color=&h0F7FFE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="INASVVN" \ 
     misc_feature    6484..6486
                     /label=R815
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="R" \ 
     misc_feature    6490..6492
                     /label=F817 (HexaPro)
                     /SerialCloner_Color=&hFB027F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="F" \ 
     misc_feature    6715..6717
                     /label=A892 (HexaPro)
                     /SerialCloner_Color=&hFB027F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    6736..6738
                     /label=A899 (HexaPro)
                     /SerialCloner_Color=&hFB027F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    6865..6867
                     /label=A942 (HexaPro)
                     /SerialCloner_Color=&hFB027F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="direct strand translation"translation="A" \ 
     misc_feature    complement(10034..10891)
                     /label=AP(R)
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= bla gene- Ap(r) determinant  \ 
     misc_feature    complement(149..307)
                     /label=ALPHA
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= lacZ' gene encoding alpa lacZ peptide  \ 
     misc_feature    9272..9272
                     /label=ORI
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= RNaseH cleavage point  \ 
     misc_feature    complement(10034..10891)
                     /label=AmpR
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    9272..9859
                     /label=Ori
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    complement(145..307)
                     /label=lacZa
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    10892..10956
                     /label=bla promoter
                     /SerialCloner_Color=&h000000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    320..1120
                     /label=HA-L
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1130..1155
                     /label=SA
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1236..1832
                     /label=PuroR (No ATG)
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    8190..9029
                     /label=HA-R
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2360..4002
                     /label=CAG promoter
                     /SerialCloner_Color=&hFF8040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1840..2032
                     /label=SV40 LPA
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    7944..8168
                     /label=bGH Poly A
                     /SerialCloner_Color=&h00FFFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1170..1232
                     /label=2A
                     /SerialCloner_Color=&h00FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(10034..10693)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(10933..10961)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             7819..7842
                     /label=Strep tag II
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             7882..7905
                     /label=Strep tag II
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             7762..7785
                     /label=PreScission site
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    7675..7755
                     /label=Foldon
                     /SerialCloner_Color=&hFF80FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1833..1838
                     /label=XbaI (Blocked by Dam)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagaa ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg 
      361 ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc 
      421 cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc 
      481 tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc 
      541 tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc 
      601 catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc 
      661 tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt 
      721 gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt 
      781 tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg 
      841 ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg 
      901 tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc 
      961 ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg 
     1021 gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg 
     1081 gttctgggta cttttatctg tcccctccac cccacagtgg ggcaagcttc tgacctcttc 
     1141 tcttcctccc acagggcctc gagagatctg gcggcggaga gggcagagga agtcttctaa 
     1201 catgcggtga cgtggaggag aatcccggcc ctagcaccga gtacaagccc acggtgcgcc 
     1261 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 
     1321 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 
     1381 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 
     1441 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 
     1501 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 
     1561 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 
     1621 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 
     1681 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 
     1741 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 
     1801 cctggtgcat gacccgcaag cccggtgcct gatctagaca gacatgataa gatacattga 
     1861 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 
     1921 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 
     1981 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aatgcatcca 
     2041 cggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct 
     2101 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt 
     2161 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc 
     2221 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa 
     2281 cggaagccgc acgtctcact agttattaat agtaatcaat tacggggtca ttagttcata 
     2341 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     2401 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     2461 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
     2521 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     2581 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     2641 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 
     2701 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 
     2761 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 
     2821 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 
     2881 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 
     2941 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 
     3001 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 
     3061 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 
     3121 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 
     3181 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 
     3241 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 
     3301 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 
     3361 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 
     3421 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 
     3481 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 
     3541 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc 
     3601 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag 
     3661 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc 
     3721 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc 
     3781 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg 
     3841 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc 
     3901 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg 
     3961 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag 
     4021 gccgagttcg gtaccgccac cATGTTCGTG TTCCTGGTGC TCCTGCCTCT GGTGAGCAGC 
     4081 CAGTGCGTGA ACCTGACCAC CCGAACCCAG CTCCCACCAG CCTACACCAA CAGCTTTACA 
     4141 CGGGGCGTGT ACTACCCTGA CAAGGTGTTC AGATCTAGCG TCCTGCACAG CACTCAGGAC 
     4201 CTCTTCCTGC CGTTCTTCAG CAACGTGACA TGGTTCCACG CCATCCACGT GAGCGGCACA 
     4261 AACGGAACCA AGCGGTTTGA TAACCCCGTC CTGCCATTCA ATGATGGAGT TTACTTCGCC 
     4321 AGTACCGAGA AGAGTAACAT CATCCGGGGC TGGATCTTCG GCACCACCCT GGATAGCAAA 
     4381 ACACAGAGCC TCCTGATCGT GAACAATGCC ACGAACGTCG TGATCAAGGT GTGCGAGTTC 
     4441 CAGTTTTGCA ATGATCCTTT CCTGGGTGTG TACTACCACA AGAACAACAA GAGCTGGATG 
     4501 GAAAGCGAGT TCAGAGTCTA CAGCAGCGCC AACAACTGCA CATTCGAGTA CGTCTCTCAG 
     4561 CCTTTTCTGA TGGACCTTGA GGGGAAACAA GGCAACTTCA AGAACCTGAG AGAATTCGTG 
     4621 TTCAAGAACA TCGACGGCTA CTTCAAAATC TACTCCAAGC ACACACCCAT CAACCTGGTC 
     4681 CGGGACCTCC CTCAGGGCTT CAGCGCCCTG GAACCCCTGG TCGACCTGCC CATAGGCATC 
     4741 AACATAACGC GGTTCCAAAC CCTGCTGGCC CTGCATAGAT CCTACCTGAC TCCTGGCGAC 
     4801 AGCAGCAGCG GATGGACCGC CGGAGCTGCA GCCTACTATG TGGGCTACCT GCAACCTAGA 
     4861 ACCTTCCTGC TGAAGTACAA CGAGAACGGC ACAATCACAG ACGCCGTCGA CTGCGCCCTG 
     4921 GACCCTCTCT CTGAGACAAA GTGCACCCTG AAGTCCTTCA CCGTGGAAAA GGGCATCTAC 
     4981 CAGACCAGCA ACTTCCGGGT GCAGCCTACA GAGAGCATCG TGCGATTTCC AAACATTACC 
     5041 AACCTCTGCC CCTTCGGCGA GGTGTTTAAC GCCACAAGAT TTGCCTCCGT TTACGCCTGG 
     5101 AATAGAAAGA GAATCAGCAA TTGTGTGGCC GACTACTCCG TGCTGTATAA CAGCGCCTCT 
     5161 TTCAGCACCT TCAAGTGCTA CGGCGTTTCC CCAACAAAGC TGAATGACCT GTGCTTCACC 
     5221 AACGTGTACG CCGACTCCTT CGTAATTAGA GGCGATGAGG TGCGGCAGAT CGCACCAGGC 
     5281 CAGACCGGTA AGATCGCTGA CTACAACTAT AAGCTGCCTG ATGATTTTAC AGGCTGCGTG 
     5341 ATCGCCTGGA ACTCTAACAA CCTGGATAGC AAGGTGGGCG GCAACTACAA CTACCTGTAC 
     5401 CGGCTGTTTC GCAAGTCTAA CCTGAAACCT TTCGAGAGAG ACATCTCCAC AGAGATCTAC 
     5461 CAGGCCGGTT CTACACCTTG TAACGGGGTG GAAGGCTTCA ACTGTTACTT CCCTCTGCAA 
     5521 AGCTACGGCT TCCAGCCTAC CAATGGAGTC GGCTACCAGC CATACCGGGT GGTCGTGCTG 
     5581 TCCTTCGAGT TACTCCACGC CCCCGCCACC GTCTGCGGTC CTAAGAAGTC CACCAATCTG 
     5641 GTTAAGAACA AATGCGTGAA CTTCAACTTC AACGGCCTGA CCGGGACCGG CGTGCTGACC 
     5701 GAAAGCAACA AAAAGTTCCT CCCCTTCCAG CAGTTCGGCC GTGATATCGC TGACACCACA 
     5761 GATGCCGTCA GAGATCCACA GACCCTGGAA ATCCTGGATA TTACACCCTG CTCCTTCGGA 
     5821 GGAGTTTCTG TGATCACCCC CGGGACCAAT ACCAGCAACC AGGTGGCTGT GCTGTACCAA 
     5881 GATGTTAACT GCACCGAGGT TCCTGTGGCC ATCCACGCCG ATCAGCTGAC ACCTACTTGG 
     5941 AGAGTGTACT CCACTGGCTC CAATGTGTTC CAGACCAGGG CCGGATGTCT GATCGGCGCC 
     6001 GAGCACGTGA ATAACAGTTA CGAGTGCGAC ATCCCTATCG GCGCCGGCAT CTGTGCCAGC 
     6061 TACCAGACCC AGACAAACAG CCCTGGGTCT GCTTCCTCTG TAGCTAGCCA GAGCATCATC 
     6121 GCCTACACCA TGAGCCTGGG CGCAGAGAAC AGCGTGGCCT ATTCCAACAA CTCTATCGCC 
     6181 ATTCCCACCA ACTTTACAAT TAGCGTCACA ACAGAGATCC TGCCCGTGAG CATGACCAAG 
     6241 ACCAGCGTGG ACTGTACAAT GTACATCTGT GGCGACAGCA CTGAATGCAG CAACCTGCTG 
     6301 CTGCAATACG GCTCCTTTTG CACCCAACTG AACCGGGCGC TGACCGGAAT CGCCGTGGAA 
     6361 CAGGACAAAA ATACCCAGGA GGTGTTCGCC CAAGTGAAGC AGATCTACAA GACCCCACCT 
     6421 ATCAAGGACT TCGGCGGCTT TAACTTTAGC CAGATTCTCC CTGATCCTTC TAAGCCTAGC 
     6481 AAGCGGAGCC CTATCGAGGA TCTGCTGTTC AACAAGGTCA CCCTGGCCGA TGCCGGCTTT 
     6541 ATCAAACAGT ATGGCGATTG CCTGGGCGAC ATAGCCGCCA GAGATCTGAT CTGCGCCCAG 
     6601 AAATTCAACG GCCTGACAGT TCTCCCACCT CTGCTGACCG ACGAGATGAT CGCTCAGTAC 
     6661 ACCTCTGCCC TGCTGGCTGG CACCATCACA TCTGGGTGGA CATTTGGCGC CGGCCCTGCC 
     6721 CTGCAGATCC CCTTTCCCAT GCAGATGGCC TATAGATTCA ACGGAATCGG CGTGACCCAG 
     6781 AACGTGCTGT ATGAAAACCA GAAGCTGATC GCTAACCAGT TCAATTCTGC CATCGGCAAG 
     6841 ATCCAGGACT CCCTCTCCTC TACCCCTAGC GCCCTGGGCA AACTGCAGGA CGTGGTGAAT 
     6901 CAGAACGCCC AAGCCCTGAA CACCCTGGTG AAGCAGCTCA GCAGCAATTT TGGCGCCATC 
     6961 AGCTCTGTGC TGAACGATAT CCTGTCTAGA CTGGACCCTC CAGAAGCCGA AGTCCAGATC 
     7021 GATAGACTGA TCACAGGCAG ACTGCAGTCC CTGCAAACCT ACGTGACCCA ACAGCTGATC 
     7081 AGGGCCGCTG AAATAAGAGC CAGCGCCAAT CTCGCCGCTA CCAAGATGTC CGAGTGTGTG 
     7141 CTGGGACAGT CTAAAcgcgT TGACTTCTGC GGCAAAGGCT ATCACCTGAT GAGCTTCCCC 
     7201 CAGAGCGCGC CGCACGGCGT GGTGTTCCTG CATGTGACAT ACGTGCCTGC CCAAGAGAAG 
     7261 AATTTCACAA CCGCCCCTGC CATCTGCCAC GACGGCAAGG CCCACTTCCC AAGAGAGGGC 
     7321 GTTTTCGTTT CCAATGGCAC ACACTGGTTC GTGACACAAA GAAACTTCTA CGAACCCCAG 
     7381 ATTATCACCA CCGACAACAC CTTCGTGAGT GGCAATTGTG ACGTGGTCAT CGGAATCGTG 
     7441 AACAACACAG TGTACGACCC TCTGCAACCT GAGCTGGACT CTTTTAAGGA AGAGCTGGAC 
     7501 AAGTACTTTA AAAACCACAC CAGCCCCGAT GTGGACCTGG GCGACATCAG TGGCATTAAC 
     7561 GCCAGCGTGG TGAACATCCA AAAGGAAATC GACAGACTGA ACGAGGTGGC CAAGAACCTG 
     7621 AACGAGTCCC TGATCGACCT GCAGGAGCTC GGCAAATACG AGCAGGGATC CGGATACATC 
     7681 CCCGAGGCCC CCAGAGATGG CCAGGCCTAC GTGCGGAAGG ACGGCGAGTG GGTACTGCTG 
     7741 AGCACATTCC TGGGCAGATC CCTGGAGGTG CTGTTCCAGG GCCCAGGCCA TCACCACCAT 
     7801 CACCACCATC ATAGCGCCTG GTCCCACCCC CAGTTCGAGA AGGGCGGCGG TAGTGGAGGG 
     7861 GGCGGATCTG GCGGCTCAGC TTGGAGCCAC CCCCAGTTcg aaaagtgata atgactcgag 
     7921 CGGCCGcgtt taaaccctgc aggctgtgcc ttctagttgc cagccatctg ttgtttgccc 
     7981 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 
     8041 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 
     8101 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 
     8161 ctctatgggt cgacagtact aagctttact agggacagga ttggtgacag aaaagcccca 
     8221 tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag 
     8281 gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga 
     8341 acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg 
     8401 gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta 
     8461 ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg 
     8521 tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat 
     8581 aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt 
     8641 cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg 
     8701 ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta 
     8761 ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt 
     8821 aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc 
     8881 taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag 
     8941 gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc 
     9001 tacaggggtt cctggctctg ctctaagggc gaattctgca ttaatgaatc ggccaacgcg 
     9061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     9121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     9181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     9241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     9301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     9361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     9421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 
     9481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     9541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     9601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     9661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 
     9721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     9781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     9841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     9901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     9961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
    10021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
    10081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
    10141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
    10201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
    10261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
    10321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
    10381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
    10441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
    10501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
    10561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
    10621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
    10681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
    10741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
    10801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
    10861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
    10921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
    10981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 
    11041 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//