LOCUS       Exported                7422 bp ds-DNA     circular SYN 27-NOV-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Nov 30, 2020 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(5..76)
                     /label=pyl-tRNA
     misc_feature    44..46
                     /label=UAG
     misc_feature    complement(78..336)
                     /label=U6 promoter
     misc_feature    499..1086
                     /label=CMV promoter
     misc_feature    1399..1404
                     /label=kozak sequence
     CDS             1405..2838
                     /codon_start=1
                     /label=MtPylRS-478(human-opti)
                     /translation="MDKKPLNTLISATGLWMSRTGKLHKIRHHEVSKRKIYIEMECGER
                     LVVNNSRSCRAARALRHHKYRKICKHCRVSDEDLNKFLTRTNEDKSNAKVTVVSAPKIR
                     KVMPKSVARTPKPLENTAPVQTLPSESQPAPTTPISASTTAPASTSTTAPAPASTTAPA
                     PASTTAPASASTTISTSAMPASTSAQGTTKFNYISGGFPRPIPVQASAPALTKSQIDRL
                     QGLLSPKDEISLDSGTPFRKLESELLSRRRKDLKQIYAEEREHYLGKLEREITKFFVDR
                     GFLEIKSPILIPMEYIERMGIDNDKELSKQIFRVDNNFCLRPMLAPNLYNYLRKLNRAL
                     PDPIKIFEIGPCYRKESDGKEHLEEFTMLNFCQMGSGCTRENLEAIIKDFLDYLGIDFE
                     IVGDSCMVYGDTLDVMHGDLELSSAVVGPVPMDRDWGINKPWIGAGFGLERLLKVMHNF
                     KNIKRASRSESYYNGISTNL"
     polyA_signal    2867..3091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3137..3565
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3579..3909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3958..4983
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_site      4995..5365
                     /label=SV40polyA
     protein_bind    5376..5397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5627..6298
                     /label=ColE1 Origin
     gene            complement(6444..7304)
                     /label=AmpR
     promoter        complement(7305..7409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaatggcgg aaaccccggg aatctaaccc ggctgaacgg atttagagtc cattcgatct
       61 acatgatcag gtttccggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga
      121 aatactttca agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg
      181 caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt
      241 ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata tgcaaatatg
      301 aaggaatcat gggaaatagg ccctcttcct gcccgacctt cgcgatgtac gggccagata
      361 tacgccaata ttggccatta gccatattat tcattggtta tatagcataa atcaatattg
      421 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
      481 gtccaatatg accgccatgt tggcattgat tattgactag ttattaatag taatcaatta
      541 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      601 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      661 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      721 ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca
      781 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta
      841 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      901 acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      961 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata
     1021 accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1081 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     1141 atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga
     1201 ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg
     1261 cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac atacgattta
     1321 ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
     1381 ccaactgcac ggaagcttgc caccatggac aagaagcctc tgaacaccct gatctctgcc
     1441 acaggcctgt ggatgagcag gaccggcaag ctgcacaaga tccgccacca cgaggtgtcc
     1501 aagcggaaga tctacatcga gatggagtgc ggcgagcgcc tggtggtgaa caattctcgc
     1561 agctgtcggg cagcaagagc cctgaggcac cacaagtata gaaagatctg caagcactgt
     1621 agggtgtccg acgaggatct gaacaagttc ctgaccagga caaacgagga taagagcaat
     1681 gccaaggtga ccgtggtgtc cgcccccaag atcagaaaag tgatgcctaa gagcgtggcc
     1741 aggacaccca agcctctgga gaataccgcc cctgtgcaga cactgccatc cgagtctcag
     1801 ccagccccca ccacaccaat cagcgcctcc accacagcac cagcatctac cagcaccaca
     1861 gcacctgccc cagcctctac cacagcacca gcacctgcaa gcaccacagc accagcctcc
     1921 gcctctacca caatcagcac ctccgccatg cctgcctcta caagcgccca gggcaccaca
     1981 aagttcaatt acatctctgg cggctttcca aggccaatcc ctgtgcaggc atccgccccc
     2041 gccctgacca agtctcagat cgacagactc cagggcctgc tgagccctaa ggacgagatc
     2101 tccctggatt ctggcacacc attcaggaag ctggagagcg agctgctgtc ccggagaagg
     2161 aaggacctga agcagatcta cgccgaggag cgcgagcact atctgggcaa gctggagcgg
     2221 gagatcacca agttctttgt ggatagaggc tttctggaga tcaagagccc catcctgatc
     2281 cctatggagt acatcgagcg catgggcatc gacaacgata aggagctgtc caagcagatc
     2341 ttccgcgtgg ataacaattt ttgcctgcgg ccaatgctgg cccccaacct gtacaattat
     2401 ctgagaaagc tgaatagggc cctgccagac cccatcaaga tctttgagat cggcccatgt
     2461 tatcggaagg agtccgatgg caaggagcac ctggaggagt tcaccatgct gaacttttgc
     2521 cagatgggct ctggctgtac aagagagaat ctggaggcca tcatcaagga cttcctggat
     2581 tacctgggca tcgactttga gatcgtgggc gattcctgca tggtgtatgg cgacaccctg
     2641 gatgtgatgc acggcgacct ggagctgagc tccgccgtgg tgggacctgt gccaatggac
     2701 agggattggg gcatcaacaa gccctggatc ggagcaggat tcggactgga gcggctgctg
     2761 aaagtgatgc acaactttaa gaatatcaag cgcgcctccc ggtctgagag ctactataac
     2821 ggcatcagca caaatctgtg aggatccacc cgctgatcag cctcgactgt gccttctagt
     2881 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2941 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3001 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3061 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3121 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3181 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3241 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct
     3301 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     3361 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     3421 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     3481 tattcttttg atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg
     3541 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     3601 agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3661 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3841 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3901 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
     3961 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4021 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     4081 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     4141 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     4201 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     4261 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4321 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     4381 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     4441 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     4501 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     4561 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     4621 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     4681 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     4741 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     4801 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     4861 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4921 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     4981 tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5041 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5101 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5161 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5221 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5281 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     5881 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6121 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6181 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc ga
//
