LOCUS       Exported               10183 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20201201_Lenti_stgRNA-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      73..661
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    949..970
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        985..1015
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1023..1039
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1047..1063
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1084..1102
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1128..1356
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1357..1537
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1584..1709
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2202..2435
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     promoter        2842..3082
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    4972..5047
                     /note="modified scaffold_stgRNA"
     misc_feature    5103..5220
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5359..6537
                     /note="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5590..6528
                     /note="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             6550..7149
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    7165..7753
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7636..7647)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             7825..8058
                     /note="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8136..8257
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8297..8432
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8453..8471)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8481..8497)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      8639..9094
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9120..9224
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             9225..10085
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
       61 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
      721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      781 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      841 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      901 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      961 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     1021 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     1081 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     1141 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     1201 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     1261 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     1321 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     1381 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     1441 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     1501 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     1561 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     1621 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1681 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1741 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1801 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1861 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1921 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1981 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     2041 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     2101 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     2161 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     2221 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     2281 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     2341 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     2401 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     2461 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     2521 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     2581 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     2641 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2701 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2761 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2821 ccaaccccga ggggacccag agagggccta tttcccatga ttccttcata tttgcatata
     2881 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
     2941 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     3001 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     3061 ttatatatct tgtggaaagg acgaaacacc ggagacggtt gtaaatgagc acacaaaata
     3121 cacatgctaa aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa
     3181 taactttagt atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat
     3241 gagcaaaata aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat
     3301 agctactcca ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc
     3361 ttcaaatgtt ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt
     3421 cattagttta acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac
     3481 aaattctaat ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa
     3541 taaacaattt tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt
     3601 catgtcttct aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat
     3661 atcaatttct gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt
     3721 tgtgcatgca cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa
     3781 taattcttta caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc
     3841 aattgcttca tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt
     3901 gtgatctata tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat
     3961 tctttaataa tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata
     4021 aaataaattt ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct
     4081 tctaaataaa attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct
     4141 tcaacattaa caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc
     4201 attaatggtt caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa
     4261 gtgtaatatg cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt
     4321 tgttttttat aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta
     4381 tctctagctg agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt
     4441 aatccgttat cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt
     4501 tttacaatat taactccata tttttcatga gcgattctat cacccaaatc acttgttaca
     4561 aaacttgaat atagagccgg attttttgga atgctattta agcgttttag atttgataat
     4621 tttcaatcaa ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca
     4681 tgttttattg ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca
     4741 tcatgtttaa taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga
     4801 tttacaacat ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta
     4861 tatcctgatt cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta
     4921 aaaataattt ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgggttagag
     4981 ctagaaatag caagttaacc taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     5041 tcggtgcttt tttaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac
     5101 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     5161 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     5221 cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg
     5281 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
     5341 ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     5401 gtccccgaga agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc
     5461 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     5521 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     5581 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     5641 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     5701 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     5761 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     5821 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     5881 gcgacgcttt ttttctggca agatagtttt gtaaatgcgg gccaagatct gcacactggt
     5941 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     6001 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     6061 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     6121 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     6181 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     6241 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     6301 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     6361 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     6421 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     6481 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgacgt
     6541 acggccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     6601 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     6661 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     6721 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc
     6781 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6841 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6901 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     6961 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7021 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7081 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7141 ggtgcctgaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7201 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7261 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7321 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7381 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7441 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7561 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7621 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7681 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7741 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7801 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7861 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7921 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7981 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8041 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     8101 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     8161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8281 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8341 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8401 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8461 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8521 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8581 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8641 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8701 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8761 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8821 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8881 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8941 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9001 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     9061 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     9121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9241 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9301 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9361 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9421 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9481 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9541 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9601 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9661 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9721 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9781 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9841 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9901 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9961 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10021 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10081 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10141 aatttaaaag gatctaggtg aagatccttt ttgataatct cat
//
