LOCUS       Exported                9955 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20201201_pcDNA5-FRT-CMV_promoter-Cas9-E2A-mRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        174..553
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        554..757
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        802..820
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             840..4943
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4959..4979
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             4980..5006
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             5022..5081
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5088..5801
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /note="TagRFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     polyA_signal    5852..6076
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6360..6407
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6415..7437
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7567..7688
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7737..7753)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7761..7777
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7785..7815)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7830..7851
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8139..8727)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8898..9758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9759..9863)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
       61 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      121 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      181 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      241 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      301 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      361 ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac atcaagtgta
      421 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      481 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      541 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      601 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      661 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      721 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      781 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttgccacca
      841 tggacaagaa gtacagcatc ggcctggaca tcggtaccaa cagcgtgggc tgggccgtga
      901 tcaccgacga gtacaaggtg cccagcaaga agttcaaggt gctgggcaac accgaccgcc
      961 acagcatcaa gaagaacctg atcggcgccc tgctgttcga cagcggcgag accgccgagg
     1021 ccacccgcct gaagcgcacc gcccgccgcc gctacacccg ccgcaagaac cgcatctgct
     1081 acctgcagga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccaccgcc
     1141 tggaggagag cttcctggtg gaggaggaca agaagcacga gcgccacccc atcttcggca
     1201 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgcgcaaga
     1261 agctggtgga cagcaccgac aaggccgacc tgcgcctgat ctacctggcc ctggcccaca
     1321 tgatcaagtt ccgcggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
     1381 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gagaacccca
     1441 tcaacgccag cggcgtggac gccaaggcca tcctgagcgc ccgcctgagc aagagccgcc
     1501 gcctggagaa cctgatcgcc cagctgcccg gcgagaagaa gaacggcctg ttcggcaacc
     1561 tgatcgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
     1621 acgccaagct gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
     1681 agatcggcga ccagtacgcc gacctgttcc tggccgccaa gaacctgagc gacgccatcc
     1741 tgctgagcga catcctgcgc gtgaacaccg agatcaccaa ggcccccctg agcgccagca
     1801 tgatcaagcg ctacgacgag caccaccagg acctgaccct gctgaaggcc ctggtgcgcc
     1861 agcagctgcc cgagaagtac aaggagatct tcttcgacca gagcaagaac ggctacgccg
     1921 gctacatcga cggcggcgcc agccaggagg agttctacaa gttcatcaag cccatcctgg
     1981 agaagatgga cggcaccgag gagctgctgg tgaagctgaa ccgcgaggac ctgctgcgca
     2041 agcagcgcac cttcgacaac ggcagcatcc cccaccagat ccacctgggc gagctgcacg
     2101 ccatcctgcg ccgccaggag gacttctacc ccttcctgaa ggacaaccgc gagaagatcg
     2161 agaagatcct gaccttccgc atcccctact acgtgggccc cctggcccgc ggcaacagcc
     2221 gcttcgcctg gatgacccgc aagagcgagg agaccatcac cccctggaac ttcgaggagg
     2281 tggtggacaa gggcgccagc gcccagagct tcatcgagcg catgaccaac ttcgacaaga
     2341 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
     2401 acaacgagct gaccaaggtg aagtacgtga ccgagggcat gcgcaagccc gccttcctga
     2461 gcggcgagca gaagaaggcc atcgtggacc tgctgttcaa gaccaaccgc aaggtgaccg
     2521 tgaagcagct gaaggaggac tacttcaaga agatcgagtg cttcgacagc gtggagatca
     2581 gcggcgtgga ggaccgcttc aacgccagcc tgggcaccta ccacgacctg ctgaagatca
     2641 tcaaggacaa ggacttcctg gacaacgagg agaacgagga catcctggag gacatcgtgc
     2701 tgaccctgac cctgttcgag gaccgcgaga tgatcgagga gcgcctgaag acctacgccc
     2761 acctgttcga cgacaaggtg atgaagcagc tgaagcgccg ccgctacacc ggctggggcc
     2821 gcctgagccg caagcttatc aacggcatcc gcgacaagca gagcggcaag accatcctgg
     2881 acttcctgaa gagcgacggc ttcgccaacc gcaacttcat gcagctgatc cacgacgaca
     2941 gcctgacctt caaggaggac atccagaagg cccaggtgag cggccagggc gacagcctgc
     3001 acgagcacat cgccaacctg gccggcagcc ccgccatcaa gaagggcatc ctgcagaccg
     3061 tgaaggtggt ggacgagctg gtgaaggtga tgggccgcca caagcccgag aacatcgtga
     3121 tcgagatggc ccgcgagaac cagaccaccc agaagggcca gaagaacagc cgcgagcgca
     3181 tgaagcgcat cgaggagggc atcaaggagc tgggcagcca gatcctgaag gagcaccccg
     3241 tggagaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag aacggccgcg
     3301 acatgtacgt ggaccaggag ctggacatca accgcctgag cgactacgac gtggaccaca
     3361 tcgtgcccca gagcttcctg aaggacgaca gcatcgacaa caaggtgctg acccgcagcg
     3421 acaagaaccg cggcaagagc gacaacgtgc ccagcgagga ggtggtgaag aagatgaaga
     3481 actactggcg ccagctgctg aacgccaagc tgatcaccca gcgcaagttc gacaacctga
     3541 ccaaggccga gcgcggcggc ctgagcgagc tggacaaggc cggcttcatc aagcgccagc
     3601 tggtggagac ccgccagatc accaagcacg tggcccagat cctggacagc cgcatgaaca
     3661 ccaagtacga cgagaacgac aagctgatcc gcgaggtgaa ggtgatcacc ctgaagagca
     3721 agctggtgag cgacttccgc aaggacttcc agttctacaa ggtgcgcgag atcaacaact
     3781 accaccacgc ccacgacgcc tacctgaacg ccgtggtggg caccgccctg atcaagaagt
     3841 accccaagct ggagagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcgcaaga
     3901 tgatcgccaa gagcgagcag gagatcggca aggccaccgc caagtacttc ttctacagca
     3961 acatcatgaa cttcttcaag accgagatca ccctggccaa cggcgagatc cgcaagcgcc
     4021 ccctgatcga gaccaacggc gagaccggcg agatcgtgtg ggacaagggc cgcgacttcg
     4081 ccaccgtgcg caaggtgctg agcatgcccc aggtgaacat cgtgaagaag accgaggtgc
     4141 agaccggcgg cttcagcaag gagagcatcc tgcccaagcg caacagcgac aagctgatcg
     4201 cccgcaagaa ggactgggac cccaagaagt acggcggctt cgacagcccc accgtggcct
     4261 acagcgtgct ggtggtggcc aaggtggaga agggcaagag caagaagctg aagagcgtga
     4321 aggagctgct gggcatcacc atcatggagc gcagcagctt cgagaagaac cccatcgact
     4381 tcctggaggc caagggctac aaggaggtga agaaggacct gatcatcaag ctgcccaagt
     4441 acagcctgtt cgagctggag aacggccgca agcgcatgct ggccagcgcc ggcgagctgc
     4501 agaagggcaa cgagctggcc ctgcccagca agtacgtgaa cttcctgtac ctggccagcc
     4561 actacgagaa gctgaagggc agccccgagg acaacgagca gaagcagctg ttcgtggagc
     4621 agcacaagca ctacctggac gagatcatcg agcagatcag cgagttcagc aagcgcgtga
     4681 tcctggccga cgccaacctg gacaaggtgc tgagcgccta caacaagcac cgcgacaagc
     4741 ccatccgcga gcaggccgag aacatcatcc acctgttcac cctgaccaac ctgggcgccc
     4801 ccgccgcctt caagtacttc gacaccacca tcgaccgcaa gcgctacacc agcaccaagg
     4861 aggtgctgga cgccaccctg atccaccaga gcatcaccgg tctgtacgag acccgcatcg
     4921 acctgagcca gctgggcggc gacggcggct ccggacctcc aaagaaaaag agaaaagtat
     4981 acccctacga cgtgcccgac tacgccgaat tcggaagcgg gcagtgcacc aactacgccc
     5041 tgctgaagct ggccggcgac gtggagagca accccggccc cggatccatg gtgtctaagg
     5101 gcgaagagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
     5161 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     5221 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
     5281 gcttcatgta cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta
     5341 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgag gacgggggcg
     5401 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     5461 tcagaggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     5521 aggccaacac cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg
     5581 ccctgaagct cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca
     5641 agaaacccgc taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa
     5701 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat
     5761 actgcgacct ccctagcaaa ctggggcaca aacttaattg agcggccgct cgagtctaga
     5821 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5881 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5941 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6001 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6061 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6121 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6181 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6241 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6301 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtacctag
     6361 aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcctt ggccaaaaag
     6421 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
     6481 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
     6541 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
     6601 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
     6661 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
     6721 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
     6781 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
     6841 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
     6901 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
     6961 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     7021 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     7081 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     7141 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     7201 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     7261 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     7321 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     7381 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac
     7441 gtactacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     7501 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     7561 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7621 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7681 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     7741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8761 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8821 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8881 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8941 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9001 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9061 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9121 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9181 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9241 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9301 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9361 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9421 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9481 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9541 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9601 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9661 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9721 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9781 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9841 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     9901 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgc
//
