LOCUS       Exported               10381 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20180122_FRT-HygR-CMV-ciCas9-E2A-RFP_JH80124B1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10381)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10381
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    820..838
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      972..981
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             981..1004
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             2982..5218
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /note="Cas9(C)"
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNL"
     CDS             3850..3861
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     CDS             5379..5399
                     /codon_start=1
                     /locus_tag="
                     "
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /note="
                     "
                     /protein_id="
                     "
                     /translation="PKKKRKV"
     misc_feature    5415..5495
                     /note="BH3 peptide"
     misc_feature    5478..5480
                     /note="WT BH3 peptide"
     CDS             5511..5570
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /note="E2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    5511..5570
                     /label="E2A"
                     /note="E2A"
     CDS             5577..6287
                     /codon_start=1
                     /label="mRFP"
                     /note="mRFP"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     polyA_signal    6339..6563
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6847..6894
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6902..7924
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8054..8175
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8224..8240)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8248..8264
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8272..8302)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8317..8338
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9385..10245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRHIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10246..10350)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt tgccaccatg gactacaagg acgacgacga caaggacaag aagtactcca
     1021 ttgggctcga tatcggcaca aacagcgtcg gctgggccgt cattacggac gagtacaagg
     1081 tgccgagcaa aaaattcaaa gttctgggca ataccgatcg ccacagcata aagaagaacc
     1141 tcattggcgc cctcctgttc gactccgggg agacggccga agccacgcgg ctcaaaagaa
     1201 cagcacggcg cagatatacc cgcagaaaga atcggatctg ctacctgcag gagatcttta
     1261 gtaatgagat ggctaaggtg gatgactctt tcttccatag gctggaggag tcctttttgg
     1321 tggaggagga taaaaagcac gagcgccacc caatctttgg caatatcgtg gacgaggtgg
     1381 cgtaccatga aaagtaccca accatatatc atctgaggaa gaagcttgta gacagtactg
     1441 ataaggctga cttgcggttg atctatctcg cgctggcgca tatgatcaaa tttcggggac
     1501 acttcctcat cgagggggac ctgaacccag acaacagcag caaccgggag ctggtggttg
     1561 actttctctc ctacaagctt tcccagaaag gatacagctg gagtcagttt agtgatgtgg
     1621 aagagaacag gactgaggcc ccagaaggga ctgaatcgga gatggagacc cccagtgcca
     1681 tcaatggcaa cccatcctgg cacctggcag acagccccgc ggtgaatgga gccactggcc
     1741 acagcagcag tttggatgcc cgggaggtga tccccatggc agcagtaaag caagcgctga
     1801 gggaggcagg cgacgagttt gaactgcggt accggcgggc attcagtgac ctgacatccc
     1861 agctccacat caccccaggg acagcatatc agagctttga acaggtagtg aatgaactct
     1921 tccgggatgg ggtaaactgg ggtcgcattg tggccttttt ctccttcggc ggggcactgt
     1981 gcgtggaaag cgtagacaag gagatgcagg tattggtgag tcggatcgca gcttggatgg
     2041 ccacttacct gaatgaccac ctagagcctt ggatccagga gaacggcggc tgggatactt
     2101 ttgtggaact ctatgggaac aatggtagcg gaactgcttc tgggactggg agtggcaccg
     2161 gttcagcaac aggaagcggc actgtgaaca cggagatcac caaagctccg ctgagcgcta
     2221 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     2281 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     2341 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     2401 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     2461 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     2521 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     2581 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     2641 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     2701 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     2761 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     2821 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     2881 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     2941 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     3001 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     3061 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     3121 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     3181 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     3241 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
     3301 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     3361 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     3421 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     3481 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     3541 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     3601 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     3661 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     3721 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggatc
     3781 atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     3841 ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     3901 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     3961 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     4021 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     4081 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     4141 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     4201 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     4261 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     4321 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     4381 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     4441 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     4501 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     4561 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     4621 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     4681 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     4741 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     4801 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     4861 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     4921 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     4981 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     5041 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     5101 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     5161 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     5221 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     5281 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     5341 tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtga
     5401 caggaagcgg cactgctccc cccaatctct gggcagcgca gcgctacggc cgtgagctcc
     5461 gaaggatggc cgatgagggt gagggttcct tcaaggaatt cggaagcggg cagtgcacca
     5521 actacgccct gctgaagctg gccggcgacg tggagagcaa ccccggcccc ggatccatgg
     5581 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     5641 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     5701 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     5761 tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg
     5821 acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg
     5881 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     5941 acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     6001 tcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa
     6061 gcgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat
     6121 acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca
     6181 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg
     6241 tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaattaa ggccgctcga
     6301 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6361 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6421 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6481 ggggggtagg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6541 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6601 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6661 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6721 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6781 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6841 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     6901 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     6961 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     7021 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     7081 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     7141 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     7201 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     7261 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     7321 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     7381 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     7441 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     7501 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     7561 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     7621 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     7681 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     7741 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     7801 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     7861 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     7921 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7981 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8041 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     8101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8161 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     8221 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8281 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8341 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8401 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8821 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9841 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9901 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9961 atagtgtatg tggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    10021 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    10081 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    10141 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    10201 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10261 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    10321 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    10381 c
//
