LOCUS       Exported               13882 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20201201_Lenti_CMV-Cas9-P2A-HygR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        119..498
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        500..698
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             716..896
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    943..1068
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1561..1794
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2321..2438
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2492..2871
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2872..3075
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3120..3138
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3160..7263
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7264..7311
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7312..7335
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             7345..7401
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7402..8430
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MAKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    8455..9043
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8926..8937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(9046..9062)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9568..9748
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9780..10004
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10050..10478
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10492..10821
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      10672..10807
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        10869..10916
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10935..11309
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11439..11560
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11609..11625)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11633..11649
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11657..11687)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11702..11723
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12011..12599)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12770..13630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13631..13735)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
       61 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      121 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      181 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      481 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      541 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      601 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      661 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
      721 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      781 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      841 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      901 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      961 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1021 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1081 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1141 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1201 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1261 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1321 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1381 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1441 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1501 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1561 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1621 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1681 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1741 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1801 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1861 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1921 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1981 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2041 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2101 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2161 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2221 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     2281 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2341 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2401 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2461 gagatccagt ttggttaatt agctacgcgt tgacattgat tattgactag ttattaatag
     2521 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2581 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     2641 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2701 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     2761 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     2821 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     2881 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     2941 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     3001 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     3061 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     3121 aatacgactc actataggga gacccaagct gctgccacca tggacaagaa gtacagcatc
     3181 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     3241 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     3301 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     3361 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     3421 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     3481 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     3541 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     3601 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     3661 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     3721 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     3781 gccaaggcca tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc
     3841 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     3901 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     3961 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     4021 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     4081 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     4141 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     4201 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     4261 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     4321 gaactgctcg tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac
     4381 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     4441 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     4501 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     4561 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     4621 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     4681 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     4741 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     4801 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     4861 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     4921 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     4981 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     5041 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     5101 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     5161 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     5221 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     5281 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     5341 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     5401 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     5461 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     5521 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     5581 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     5641 ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg
     5701 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     5761 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     5821 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     5881 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     5941 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     6001 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     6061 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     6121 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     6181 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     6241 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     6301 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     6361 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     6421 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     6481 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     6541 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     6601 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctact ggggatcacc
     6661 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     6721 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     6781 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     6841 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     6901 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     6961 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     7021 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     7081 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     7141 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     7201 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggc
     7261 gacaagcgac ctgccgccac aaagaaggct ggacaggcta agaagaagaa agattacaaa
     7321 gacgatgacg ataagggatc cggcgcaaca aacttctctc tgctgaaaca agccggagat
     7381 gtcgaagaga atcctggacc gatggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     7441 aagtttctga tcgaaaagtt cgacagcgtg tccgacctga tgcagctctc ggagggcgaa
     7501 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     7561 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     7621 ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta ttgcatctcc
     7681 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     7741 cagccggtcg cggaggcaat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     7801 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     7861 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     7921 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     7981 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     8041 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     8101 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     8161 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     8221 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     8281 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     8341 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     8401 cccagcactc gtccgagggc aaaggaatga gaattcgata tcaagcttat cggtaatcaa
     8461 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8521 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8581 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8641 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8701 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8761 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8821 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8881 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8941 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9001 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     9061 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     9121 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     9181 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     9241 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     9301 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     9361 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     9421 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     9481 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     9541 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     9601 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9661 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9721 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     9781 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     9841 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     9901 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     9961 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
    10021 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
    10081 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    10141 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    10201 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    10261 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    10321 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    10381 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    10441 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
    10501 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10621 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10741 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
    10801 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
    10861 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
    10921 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
    10981 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
    11041 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
    11101 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
    11161 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
    11221 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
    11281 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
    11341 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    11401 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    11461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    11521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    11581 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    11641 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11701 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    11761 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    11821 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    12001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    12061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    12121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    12181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    12241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    12301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    12361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    12421 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    12481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    12541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    12601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    12721 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12781 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    12841 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    12901 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    12961 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    13021 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    13081 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    13141 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    13201 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    13261 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    13321 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    13381 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    13441 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    13501 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    13561 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    13621 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    13681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    13741 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
    13801 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
    13861 tgtgttggag gtcgctgagt ag
//
