LOCUS       Exported               13724 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20201201_Lenti_PGK-Cas9-P2A-HygR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   jihye
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        119..498
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        500..698
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             716..896
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    943..1068
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1561..1794
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2321..2438
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2488..2987
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3002..7105
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /note="Cas9"
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7106..7153
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7154..7177
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     CDS             7187..7243
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7244..8272
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MAKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     misc_feature    8297..8885
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8768..8779)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(8888..8904)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             9410..9590
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9622..9846
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9892..10320
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10334..10663
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      10514..10649
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        10711..10758
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             10777..11151
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11281..11402
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11451..11467)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11475..11491
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11499..11529)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    11544..11565
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11853..12441)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12612..13472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13473..13577)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
       61 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      121 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      181 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      481 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      541 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      601 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      661 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
      721 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      781 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      841 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      901 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      961 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1021 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1081 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1141 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1201 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1261 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1321 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1381 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1441 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1501 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1561 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1621 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1681 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1741 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1801 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1861 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1921 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1981 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2041 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2101 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2161 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2221 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
     2281 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     2341 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     2401 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     2461 gagatccagt ttggttaatt agctagcggg taggggaggc gcttttccca aggcagtctg
     2521 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     2581 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     2641 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     2701 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     2761 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     2821 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     2881 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     2941 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgata gcgctgccac
     3001 catggacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
     3061 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     3121 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     3181 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     3241 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     3301 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     3361 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     3421 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     3481 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     3541 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     3601 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     3661 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     3721 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     3781 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     3841 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     3901 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     3961 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     4021 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     4081 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     4141 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     4201 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     4261 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     4321 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     4381 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     4441 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     4501 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     4561 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     4621 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     4681 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     4741 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     4801 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     4861 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     4921 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     4981 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     5041 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     5101 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     5161 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     5221 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     5281 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     5341 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     5401 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     5461 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca
     5521 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     5581 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     5641 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     5701 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     5761 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     5821 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     5881 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     5941 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     6001 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     6061 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     6121 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     6181 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     6241 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     6301 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     6361 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     6421 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     6481 gaaagagcta ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     6541 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     6601 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     6661 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     6721 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     6781 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     6841 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     6901 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     6961 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     7021 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     7081 cgacctgtct cagctgggag gcgacaagcg acctgccgcc acaaagaagg ctggacaggc
     7141 taagaagaag aaagattaca aagacgatga cgataaggga tccggcgcaa caaacttctc
     7201 tctgctgaaa caagccggag atgtcgaaga gaatcctgga ccgatggcca aaaagcctga
     7261 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tgtccgacct
     7321 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     7381 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     7441 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agttcagcga
     7501 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     7561 aaccgaactg cccgctgttc tgcagccggt cgcggaggca atggatgcga tcgctgcggc
     7621 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     7681 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     7741 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     7801 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     7861 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     7921 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     7981 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     8041 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     8101 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     8161 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     8221 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat gagaattcga
     8281 tatcaagctt atcggtaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     8341 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8401 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8461 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8521 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8581 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8641 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8701 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8761 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8821 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8881 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     8941 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     9001 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     9061 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9121 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     9181 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     9241 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     9301 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     9361 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     9421 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9481 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9541 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     9601 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     9661 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     9721 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     9781 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     9841 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     9901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     9961 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10021 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10081 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10141 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10201 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10261 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10321 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10381 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10441 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10501 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10561 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    10621 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    10681 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    10741 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
    10801 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
    10861 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
    10921 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
    10981 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
    11041 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
    11101 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
    11161 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    11221 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    11281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11401 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    11461 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11521 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11581 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11641 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11701 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11761 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11821 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    11881 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    11941 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12001 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12061 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12121 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12181 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12241 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12301 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12361 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12421 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12481 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12541 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12601 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12661 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12721 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12781 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12841 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    12901 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    12961 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13021 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13081 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13141 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13201 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13261 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13321 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13381 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13441 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13501 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13561 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    13621 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    13681 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtag
//
