LOCUS       Exported                9864 bp ds-DNA    circular SYN 01-12-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    20201201_Lenti-pHSE-Cre-mRuby3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   CATSKIN
  TITLE     Direct Submission
  JOURNAL   Exported 2020¤¤¤Å¤¤ 12 1¤·¤Ó¤© ¤¾¤È¤·¤Ë¤·¤Ó¤© from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        238..617
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2440..2557
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2611..2625
                     /note="HSE"
     misc_feature    2630..2644
                     /note="HSE"
     misc_feature    2649..2663
                     /note="HSE"
     promoter        2668..2699
                     /note="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    2715..3761
                     /note="Cre"
     CDS             2715..2735
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             3762..3788
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     CDS             3804..3857
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3858..4571
                     /note="mRuby3"
     misc_feature    4596..5184
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5067..5078)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     primer_bind     complement(5187..5203)
                     /note="KS primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5709..5889
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5921..6145
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6191..6619
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6633..6922
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      6806..6908
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        6970..7017
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             7036..7410
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7540..7661
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7710..7726)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7734..7750
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7758..7788)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7803..7824
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8112..8700)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8871..9731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9732..9836)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgtcg agaacgttct agaacgtcga gaacgttcta
     2641 gaacgtcgag aacgttctag aacgtcgtag agggtatata atggaagctc gaattccaga
     2701 ccggtgccac catgcccaag aagaagagga aggtgtccaa tctcctgact gttcaccaga
     2761 acctccctgc gctgccagta gatgccacta gcgatgaggt caggaaaaat ctcatggata
     2821 tgtttaggga tagacaggcg ttttctgaac acacctggaa aatgctgctt agcgtgtgcc
     2881 gatcctgggc agcctggtgt aagctgaaca atcgcaaatg gttccccgcc gagccggagg
     2941 acgtgcgcga ttacctgctg tatctccagg caagagggct ggctgtcaag actatccagc
     3001 agcacttggg ccaactgaat atgctgcatc gacgcagcgg gctcccccgg cctagcgatt
     3061 caaacgcagt ctcccttgtt atgaggagaa ttagaaagga aaacgtagat gcgggtgaga
     3121 gggctaagca ggctctcgct tttgagcgga ctgatttcga ccaggtcaga tccctgatgg
     3181 agaacagcga tcggtgccag gacatcagga acctcgcatt tctgggaatt gcatataaca
     3241 cacttctgcg catagctgag atcgcccgga tcagagtgaa agacatcagt cgaacggacg
     3301 gcggccggat gcttattcat attggacgca caaagacatt ggtcagcacc gctggcgttg
     3361 aaaaggcctt gtccctgggc gtaacgaagc tggtggaaag atggatctca gtgtccggcg
     3421 tggctgacga ccctaataat tacttgttct gtcgagtgag aaaaaacgga gtcgccgcgc
     3481 cctctgccac cagccaattg agtacacggg cccttgaagg gatctttgag gcaacccacc
     3541 gactcatata cggagccaag gatgacagtg gccagaggta tctcgcctgg tcaggtcatt
     3601 ctgctagggt gggggccgca cgagacatgg cgcgggcagg agtctccata ccagagatta
     3661 tgcaagctgg aggttggaca aatgtgaaca tcgttatgaa ctatatccgc aatcttgact
     3721 ctgaaaccgg ggccatggtg agactgctcg aagatggtga ctacccatac gatgttccag
     3781 attacgctgg atccggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg
     3841 aggagaatcc tggcccaatg gtgtctaagg gcgaagagct gatcaaggaa aatatgcgta
     3901 tgaaggtggt catggaaggt tcggtcaacg gccaccaatt caaatgcaca ggtgaaggag
     3961 aaggcagacc gtacgaggga actcaaacca tgaggatcaa agtcatcgag ggaggacccc
     4021 tgccatttgc ctttgacatt cttgccacgt cgttcatgta tggcagccgt acttttatca
     4081 agtacccggc cgacatccct gatttcttta aacagtcctt tcctgagggt tttacttggg
     4141 aaagagttac gagatacgaa gatggtggag tcgtcaccgt cacgcaggac accagccttg
     4201 aggatggcga gctcgtctac aacgtcaagg tcagaggggt aaactttccc tccaatggtc
     4261 ccgtgatgca gaagaagacc aagggttggg agcctaatac agagatgatg tatccagcag
     4321 atggtggtct gagaggatac actgacatcg cactgaaagt tgatggtggt ggccatctgc
     4381 actgcaactt cgtgacaact tacaggtcaa aaaagaccgt cgggaacatc aagatgcccg
     4441 gtgtccatgc cgttgatcac cgcctggaaa ggatcgagga gagtgacaat gaaacctacg
     4501 tagtgcaacg cgaagtggca gttgccaaat acagcaacct tggtggtggc atggacgagc
     4561 tgtacaagta agaattcgat atcaagctta tcggtaatca acctctggat tacaaaattt
     4621 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4681 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4741 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4801 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4861 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4921 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4981 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5041 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5101 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5161 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg
     5221 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5281 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5341 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5401 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5461 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     5521 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     5581 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     5641 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     5701 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5761 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5821 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5881 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5941 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6001 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6061 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6121 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     6181 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6241 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6301 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6361 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6421 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6481 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6541 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6601 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6721 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6781 cagcaaccat agtcccgccc ctaactctcc gccccatggc tgactaattt tttttattta
     6841 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6901 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6961 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7021 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     7081 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     7141 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     7201 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     7261 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     7321 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     7381 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     7441 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7501 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     7561 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7621 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     7681 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7801 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7861 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7921 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7981 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8041 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8101 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8161 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8221 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8281 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8341 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8401 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8461 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8521 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8581 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8641 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8701 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8761 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8821 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8881 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8941 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9001 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9061 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9121 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9181 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9241 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9301 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9361 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9421 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9481 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9541 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9601 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9661 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9721 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9781 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9841 tttccccgaa aagtgccacc tgac
//
